Pre-clinical Evaluation of the Hypotensive and Anti Atherogenic Activity of Hydroethanolic Extract of Eribroma oblongum (Malvaceae) Stem Bark on Wistar Rats Models

Tsague, M.; Fokunang, Charles; Ngameni, Bathélémy; Tembe-Fokunang, Estella; Guedje, Nicole Marie; Tom, Esther Ngo Lemba; Dongmo, Faustin; Ngoupayo, Joseph; Sokeng, Sélestin Dongmo; Djomeni, Dzeufiet; Oben, Julius E.; Dimo, Théophile; Minkande, Jacqueline Zé; Tchaleu, Bonaventure Ngadjui

doi:10.9734/bjpr/2015/12782

Cited by 5 publications

(7 citation statements)

References 16 publications

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“…These resultat suggest that BA may act throw the endothelium-mediated/nitric oxide. However propranolol, (the beta blocker may oppose the vascular smooth muscle relaxation induced by the activation of the beta 2 receptor by endogenous epinephrine) appears to potentialize significantly P<0.05 the immediate hypotensive effects of BA [22], [23]. The pretreatment of normotensive rats with reserpine (5mg/kg), (inhibitor of vesicular storage of biogenic amines) were admisnistered 3 days before the plant extract administration, yohimbine, (a selective α 2 -adrenoceptor) antagonist, atropine, (the muscarinic receptor) antagonist and L-NAME induced 5 minutes after the compound 1 did not modify the immediate hypotensive effect of BA.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark

Tsague¹,

Ntungwen²,

Talla³

et al. 2016

JDMP

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Abstract:The stem bark of Eribroma oblongum (malvaceae) is used in traditional Cameroonian medicine to treat various metabolic illnesses including the management of hypertension but there is no scientific evidence to how relief is brought about. The present study was to evaluate the effect of the ethyl acetate extract of the dried stem bark of E. oblongum on arterial blood pressure and heart rate in normotensive rat (NTR) and their mechanisms of EAEO. The effects of ethyl acetate extract of Eribroma oblongum (EAEO; 10, 20, 30 mg/kg; i.v) was tested on systolic blood pressure (SBP) and heart rate (HR) of normotensive rat. The mechanism of EAEO (20mg/kg) was studied in the presence of atropine, yohimbine, propranolol, L-NAME or reserpine. At the end of the experiment, SBP and HR were recorded. EAEO (10-20 mg/kg) induced a significant hypotensive effect of SBP. The hypotensive effects of EAEO (20 mg/kg) were inhibited by pre-treatment of rats with atropine, reserpine, yohimbine and L-NAME. At the end of this study the result demonstrates that the hypotensive as well as the antihypertensive effects of the ethyl acetate extract of the stem bark of Eribroma oblongum. Our data validate the use of the extract in traditional medicine against hypertension. The effect on blood pressure is, at least in part, due to a modulation of the orthosympathetic nervous system and to the improvement of the antioxidant status. Further studies are from now needed to study the toxicity of Eribroma oblongum.

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Section: Discussionmentioning

confidence: 99%

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of Eribroma oblongum (Malvaceae) Stem Bark

Tsague¹,

Ntungwen²,

Talla³

et al. 2016

JDMP

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“…Fresh stem bark of Eribroma oblongum were collected at Eseka, Centre province of Cameroon, in August 2013. The plant material was identified at the National Herbarium (HNC) of Yaoundé-Cameroon where a voucher specimen N°27489SRFCam has been deposited [6].…”

Section: Plant Materialsmentioning

confidence: 99%

“…The crude extract was then airdried to obtain a solid mass with a yield of 25%. The extracts were then kept separately in small bottles and put in the fridge for later use [6].…”

Section: Drying and Preparation Of The Extract Of Heeomentioning

confidence: 99%

“…It is widely used in African traditional medicine to treat various metabolic illnesses including cardiovascular diseases. In Cameroon, decoction of E. oblongum dried stem bark is used by traditional healers of Centre Region, for the management of cardiovascular diseases, especially hypertension in combination with other plants [6]. Phytochemical investigations have shown the presence phytochemical analysis revealed the presence of reduced sugar, flavonoides, tannins, phenols and saponins.…”

Section: Introductionmentioning

confidence: 99%

“…Phytochemical investigations have shown the presence phytochemical analysis revealed the presence of reduced sugar, flavonoides, tannins, phenols and saponins. Terpenoides, alkaloids, lipids, steroid cardiac glycosides, anthraquinones, and triterpenes were absent [6]. The present investigation was undertaken to examine the antihypertensive activities of the stem bark hydroethanolic extract of E. oblongum in L-NAME-induced hypertensive rats (LNHR).…”

Section: Introductionmentioning

confidence: 99%

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Hydroethanolic Extract of Eribroma oblongum (Malvaceae) Stem Bark Prevents Hypertension, Oxidative Stress and Dyslipidemia in L-NAME Induced Hypertension in Wistar Rats

Valentine¹

2016

JDMP

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Introduction: Eribroma oblongum (Malvaceae) stem bark is used in Cameroonian ethnomedicine to treat various metabolic illnesses including cardiovascular diseases. The present study was designed to investigate the mechanisms of hypotensive effects of hydroethanolic extract of Eribroma oblongum and its cardioprotective effects after a subchronic treatment. Study Design: This was a prospective cross-sectional analytical study Methodology: Preventive effects were studied after oral administration of hydroethanolic extract of Eribroma oblongum (HEEO) (100 mg/kg body weight/day) or captopril (25 mg/kg body weight/day) simultaneously with L-NAME (40 mg/kg body weight/ day) in rats for 28 days. Body weight and food intake were measured after 5 days. At the end of treatment, blood pressure and heart rate were recorded. Aorta, heart, liver and kidneys were weighted. Reduced nitrite, superoxide dismutase, catalase, gluthatione, Malondialdehyde and lipid profile were measured. Results: The extract prevented L-NAME hypertension and improved food intake and body weight of rats. HEEO improved lipid profile and antioxidant status. L-NAME caused a significant (P<0.001) increase in the levels of serum transaminase relative to the normal. L-NAME treated rats had markedly decreased catalase (CAT), superoxide dismutase (SOD), nitrite (NO 2-) and reduced glutathione (GSH) levels in the tissue. Also, L-NAME caused a significant (P<0.001) induction of lipid peroxidation (MDA) in the animals tissue relative to the normal range. Administration of HEEO with L-NAME caused significant (P<0.001) inhibition of MDA relative to LNHR and augmented tissue antioxidant indices. Conclusion: The hydroethanolic stem bark extract of Eribroma oblongum showed a promising potential cardioprotective effect in L-NAME induced hypertension. This effect could be due at least in part by improving endothelial function, lipid profile and oxidative status. These findings justify the traditional use of HEEO as treatment in cardiovascular disorders and serve as a promising traditional pharmacopoeia for the development of new chemical entity in the phytomedicine development platform.

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In Vitro Antioxidant Properties and Interleukin-17 Expression Levels of Eribroma oblongum (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver

Valentine,

Jacky,

Alphonse

et al. 2024

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Introduction: Eribroma oblongum (Malvaceae) (EO) stem bark is also used in traditional medicine to treat cardiovascular diseases and painful menstruation. This study aimed to estimate the mineral composition, in vitro antioxidant properties, and Interleukin-17 (IL-17) expression levels ˂ of hydroethanolic extract (EOHE) and the powder fraction 125µm (EOP) of Eribroma oblongum (EO), in Wistar rats with atherogenic diet-induced steatotic liver. Materials and methods: The mineral composition of these therapeutic plants was characterized through atomic absorption spectrometry. Antioxidant activities were measured using spectroscopic methods. Rats were given daily doses of EOP, EOHE (100mg/kg), or Atorvastatin (10mg/kg) as a positive control for 45 days at the same time as being fed an atherogenic diet. At the end of the treatment period, animals were sacrificed and liver and blood were collected for evaluation of biochemical parameters. The serum levels of cytokines IL-17 were measured by ELISA. Results: The results showed that EOP had a significantly ˂ P 0.0001 higher composition of Iron (25.75±0.6464g/100g DM), Zinc (11.71±7164g/100g DM), Vitamin C (41.60 ± 4.864g/100g DM), Manganese (2.02±0.0164g/100g DM), Selenium (0.037±0.000764g/100g DM) than EOHE. EOHE had a significantly higher polyphenol content (p<0.05) than EOP 917.33±23.63µg CaE/g DM. EOP and EOHE had good polyphenol contents (in descending order EOHE (995.43±24.10 (µg CaE/g DM)) > EOP(917.33±23.63 (µg CaE/g DM)). DPPH, FRAP, and TAC increased positively with hydroethanolic extract and powder concentration. EOP significantly ( ˂ P 0.0001) reduced IL-17 expression by 48,76% compared with the negative control group. Conclusion: These results demonstrated that EOP can be a source of anti-inflammatory agents in the fight against metabolic diseases. The therapeutic potential of Eribroma oblongum for the treatment of steatosis could be considered in further studies.

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Pre-clinical Evaluation of the Hypotensive and Anti Atherogenic Activity of Hydroethanolic Extract of Eribroma oblongum (Malvaceae) Stem Bark on Wistar Rats Models

Cited by 5 publications

References 16 publications

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of <i>Eribroma oblongum </i> (Malvaceae) Stem Bark

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of <i>Eribroma oblongum </i> (Malvaceae) Stem Bark

Hydroethanolic Extract of <i>Eribroma oblongum</i> (Malvaceae) Stem Bark Prevents Hypertension, Oxidative Stress and Dyslipidemia in L-NAME Induced Hypertension in Wistar Rats

<i>In Vitro</i> Antioxidant Properties and Interleukin-17 Expression Levels of <i>Eribroma oblongum</i> (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver

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Pre-clinical Evaluation of the Hypotensive and Anti Atherogenic Activity of Hydroethanolic Extract of Eribroma oblongum (Malvaceae) Stem Bark on Wistar Rats Models

Cited by 5 publications

References 16 publications

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of &lt;i&gt;Eribroma oblongum &lt;/i&gt; (Malvaceae) Stem Bark

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of &lt;i&gt;Eribroma oblongum &lt;/i&gt; (Malvaceae) Stem Bark

Hydroethanolic Extract of &lt;i&gt;Eribroma oblongum&lt;/i&gt; (Malvaceae) Stem Bark Prevents Hypertension, Oxidative Stress and Dyslipidemia in L-NAME Induced Hypertension in Wistar Rats

&lt;i&gt;In Vitro&lt;/i&gt; Antioxidant Properties and Interleukin-17 Expression Levels of &lt;i&gt;Eribroma oblongum&lt;/i&gt; (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver

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Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of <i>Eribroma oblongum </i> (Malvaceae) Stem Bark

Identification of the Bioactive Compounds Hypotensive Effect in the Ethyl Acetate Extract of <i>Eribroma oblongum </i> (Malvaceae) Stem Bark

Hydroethanolic Extract of <i>Eribroma oblongum</i> (Malvaceae) Stem Bark Prevents Hypertension, Oxidative Stress and Dyslipidemia in L-NAME Induced Hypertension in Wistar Rats

<i>In Vitro</i> Antioxidant Properties and Interleukin-17 Expression Levels of <i>Eribroma oblongum</i> (Malvaceae) in Wistar Rats with Atherogenic Diet-Induced Steatotic Liver