Pre-clinical Characterization of Tissue Engineering Constructs for Bone and Cartilage Regeneration

Trachtenberg, Jordan E.; Vo, Tiffany N.; Mikos, Antonios G.

doi:10.1007/s10439-014-1151-0

Cited by 24 publications

(24 citation statements)

References 131 publications

(163 reference statements)

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“…Specifically, the ability to quantitatively and qualitatively evaluate the dispersion of particles and prove the presence of a gradient can be a challenge when working with nanoparticles. Micro-computed tomography (µCT) is a non-destructive technique that provides a full 3D reconstruction of the scaffold and gives a better summary of particle distribution and aggregation [25, 26] than techniques that primarily characterize the surface, such as atomic force microscopy (AFM) [27] or Fourier transform-infrared spectroscopy (FT-IR) [28]. This can also be coupled with quantitative techniques like thermogravimetric analysis (TGA) to quantify concentrations within scaffold layers or within cross-sections to prove the presence of a gradient or a uniform particle distribution.…”

Section: Introductionmentioning

confidence: 99%

Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients

Trachtenberg

Placone

Smith

et al. 2017

Journal of Biomaterials Science, Polymer Edition

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101

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The primary focus of this work is to present the current challenges of printing scaffolds with concentration gradients of nanoparticles with an aim to improve the processing of these scaffolds. Furthermore, we address how print fidelity is related to material composition and emphasize the importance of considering this relationship when developing complex scaffolds for bone implants. The ability to create complex tissues is becoming increasingly relevant in the tissue engineering community. For bone tissue engineering applications, this work demonstrates the ability to use extrusion-based printing techniques to control the spatial deposition of hydroxyapatite (HA) nanoparticles in a 3D composite scaffold. In doing so, we combined the benefits of synthetic, degradable polymers, such as poly(propylene fumarate) (PPF), with osteoconductive HA nanoparticles that provide robust compressive mechanical properties. Furthermore, the final 3D printed scaffolds consisted of well-defined layers with interconnected pores, two critical features for a successful bone implant. To demonstrate a controlled gradient of HA, thermogravimetric analysis was carried out to quantify HA on a per-layer basis. Moreover, we non-destructively evaluated the tendency of HA particles to aggregate within PPF using micro-computed tomography (µCT). This work provides insight for proper fabrication and characterization of composite scaffolds containing particle gradients and has broad applicability for future efforts in fabricating complex scaffolds for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients

Trachtenberg

Placone

Smith

et al. 2017

Journal of Biomaterials Science, Polymer Edition

Self Cite

101

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“…45 On the other hand, bone tissue regeneration normally happens in a few weeks, our PPF-co-PLGA porous scaffold with 7-8 weeks sustenance in vitro should be perfect to meet the supportive demands of a defective tissue growth before full degradation. 46,47 After surface bone formation, these PPF-co-PLGA porous scaffolds were expected to gradually biodegrade, therefore giving more free space for a higher volume of bone to grow in, and thus facilitate tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional porous poly(propylene fumarate)‐co‐poly(lactic‐co‐glycolic acid) scaffolds for tissue engineering

Liu²,

Zhou³

et al. 2018

J Biomedical Materials Res

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Three-dimensional structural scaffolds have played an important role in tissue engineering, especially broad applications in areas such as regenerative medicine. We have developed novel biodegradable porous poly(propylene fumarate)-co-poly(lactic-co-glycolic acid) (PPF-co-PLGA) scaffolds using thermally induced phase separation, and determined the effects of critical parameters such as copolymer concentration (6, 8, and 10 wt %) and the binary solvent ratio of dioxane:water (78/22, 80/20, 82/18 wt/wt %) on the fabrication process. The cloud-point temperatures of PPF-co-PLGA changed in parallel with increasing copolymer concentration, but inversely with increasing dioxane content. The compressive moduli of the scaffolds increased with greater weight composition and dioxane:water ratio. Scaffolds formed using high copolymer concentrations and solvent ratios exhibited preferable biomineralization. All samples showed biodegradation capability in both accelerated solution and phosphate-buffered saline (PBS). Cell toxicity testing indicated that the scaffolds had good biocompatibility with bone and nerve cells, which adhered well to the scaffolds. Variations in the copolymer concentration and solvent ratio exercised a remarkable influence on morphology, mechanical properties, biomineralization, and biodegradation, but not on the cell viability and adhesion of the cross-linked scaffolds. An 8 to 10 wt % solute concentration and 80/20 to 82/18 wt/wt dioxane:water ratio were the optimum parameters for scaffold fabrication. PPF-co-PLGA scaffolds thus possess several promising prospects for tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2507-2517, 2018.

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“…By using realtime and non-invasive techniques, the evaluation of live tissues in vivo could permit a better understanding of in situ regeneration dynamically. In vivo non-invasive evaluation technologies include functional mechanical testing (assessing the tensile, shear, and compressive properties of engineered cartilage), imaging technologies, and cell and growth factor tracking in animal models [78]. Indentation testing is a compressive test that offers a new method for in situ, nondestructive mechanical analysis of cartilage, which aims to quantify some biomechanical characteristics [79].…”

Section: Perspectives Integration Of In Situ Imaging Technology With mentioning

confidence: 99%

Perspectives on Animal Models Utilized for the Research and Development of Regenerative Therapies for Articular Cartilage

et al. 2016

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Animal models are integral and indispensable for biomedical research and regenerative medicine studies, as these provide invaluable information for systemically evaluating the potential risks and efficacy of newly developed biomaterials, drugs, medical devices, and therapeutic modalities, prior to initiation of human clinical trials. Nevertheless, it is important to be aware of the unique strengths and limitations of the various different small and large animal models commonly utilized for biomedical research as well as the various challenges faced in extrapolating results acquired from animal studies and the risks of data misinterpretation. This review will thus critically examine various animal models utilized for studies on articular cartilage regeneration. Particular emphasis will be placed on comparing and analyzing the unique strengths and limitations of each animal model, with the aim of establishing principles for evaluating the suitability of different animal models for individual studies as well as for comprehensive interpretation and extrapolation of results obtained from various animal species. Additionally, this review will also discuss to evaluate animal studies with in situ imaging techniques, how the animal genome may result in variability in experimental outcomes, as well as the contribution of animal models to the development of cartilage tissue engineering.

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Pre-clinical Characterization of Tissue Engineering Constructs for Bone and Cartilage Regeneration

Cited by 24 publications

References 131 publications

Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients

Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients

Three‐dimensional porous poly(propylene fumarate)‐co‐poly(lactic‐co‐glycolic acid) scaffolds for tissue engineering

Perspectives on Animal Models Utilized for the Research and Development of Regenerative Therapies for Articular Cartilage

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