pRB and E2F4 play distinct cell-intrinsic roles in fetal erythropoiesis

Zhang, Jing; Lee, Eunice; Liu, Yangang; Berman, Seth D.; Lodish, Harvey F.; Lees, Jacqueline A.

doi:10.4161/cc.9.2.10467

Cited by 12 publications

(14 citation statements)

References 30 publications

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“…Thus far, the biological performance of GO on erythroid progenitor cells has not been investigated. We here assessed the impact of GO exposure on primary E14.5 fetal liver cells, which are predominantly erythroid progenitor cells with a small portion of other types of cells, such as macrophages [19,27,28]. GO provoked the substantial cell death of E14.5 fetal liver cells via apoptosis, as shown in Figure 5A, the percentages of Q4 (early apoptosis) plus Q2 (late apoptosis) were significantly increased in GO-treated cells (at 20 μg/ml, P < 0.05) compared to the control cells.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages

Wang

et al. 2013

Nanoscale Res Lett

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Great concerns have been raised about the exposure and possible adverse influence of nanomaterials due to their wide applications in a variety of fields, such as biomedicine and daily lives. The blood circulation system and blood cells form an important barrier against invaders, including nanomaterials. However, studies of the biological effects of nanomaterials on blood cells have been limited and without clear conclusions thus far. In the current study, the biological influence of quantum dots (QDs) with various surface coating on erythroid cells and graphene oxide (GO) on macrophages was closely investigated. We found that QDs posed great damage to macrophages through intracellular accumulation of QDs coupled with reactive oxygen species generation, particularly for QDs coated with PEG-NH2. QD modified with polyethylene glycol-conjugated amine particles exerted robust inhibition on cell proliferation of J744A.1 macrophages, irrespective of apoptosis. Additionally, to the best of our knowledge, our study is the first to have demonstrated that GO could provoke apoptosis of erythroid cells through oxidative stress in E14.5 fetal liver erythroid cells and in vivo administration of GO-diminished erythroid population in spleen, associated with disordered erythropoiesis in mice.

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Section: Resultsmentioning

confidence: 99%

Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages

Wang

et al. 2013

Nanoscale Res Lett

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“…They are expressed from a doxycycline (dox)inducible vector allowing for unlimited growth in the presence of EPO, SCF, and dox, and differentiation in the presence of EPO but without dox and SCF. Lack of p53 does not affect differentiation, but retinoblastoma is required for terminal erythroid differentiation [103][104][105]. Dox-inducible expression was also used to establish immortalized erythroid cells lines from CB (HUDEP) and from adult EBLs cultured from CD34 + HSPC (BEL-A) [106,107].…”

Section: Human Cell Linesmentioning

confidence: 99%

Erythropoiesis and Megakaryopoiesis in a Dish

Varga¹,

Hansen²,

Akker³

et al. 2019

Cell Culture

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Erythrocytes and platelets are the major cellular components of blood. Several hereditary diseases affect the production/stability of red blood cells (RBCs) and platelets (Plts) resulting in anemia or bleeding, respectively. Patients with such disorders may require recurrent transfusions, which bear a risk to develop alloantibodies and ultimately may result in transfusion product refractoriness. Cell culture models enable to unravel disease mechanisms, and to screen for alternative therapeutic products. Besides these applications, the ultimate goal is the large-scale production of blood effector cells for transfusion. Cultured RBCs that lack many of the common blood group antigens and Plts-lacking HLA expression would improve transfusion practice. Large numbers of RBCs and Plts can already be generated using hematopoietic stem cells derived from fetal liver, cord blood, peripheral blood, and bone marrow as starting material for cell culture. The recent advances to generate blood cells from induced pluripotent stem cells provide a donor-independent, immortal primary source for cell culture models. This enables us to study developmental switches during erythropoiesis/megakaryopoiesis and provides potential future therapeutic applications. In this review, we will discuss how erythropoiesis and megakaryopoiesis are mimicked in culture systems and how these models relate to the in vivo process.

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“…The first is retinoblastoma (Rb), which regulates the G1-to-S-phase transition of the cell cycle. Targeted disruption of Rb results in fetal anemia, though its preferential requirement in definitive erythroid versus macrophage cells remains controversial (54, 79–81). The second is Tropomodulin 3 (Tmod3) that binds tropomyosins and regulates the length and stability of actin filaments.…”

Section: Interactions Of Macrophage Cells and Definitive Erythroblastsmentioning

confidence: 99%

Interaction of the Macrophage and Primitive Erythroid Lineages in the Mammalian Embryo

Palis

2017

Front. Immunol.

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Two distinct forms of erythropoiesis, primitive and definitive, are found in mammals. Definitive erythroid precursors in the bone marrow mature in the physical context of macrophage cells in “erythroblastic islands.” In the murine embryo, overlapping waves of primitive hematopoietic progenitors and definitive erythro-myeloid progenitors, each containing macrophage potential, arise in the yolk sac prior to the emergence of hematopoietic stem cells. Primitive erythroblasts mature in the bloodstream as a semi-synchronous cohort while macrophage cells derived from the yolk sac seed the fetal liver. Late-stage primitive erythroblasts associate with macrophage cells in erythroblastic islands in the fetal liver, indicating that primitive erythroblasts can interact with macrophage cells extravascularly. Like definitive erythroblasts, primitive erythroblasts physically associate with macrophages through α4 integrin–vascular adhesion molecule 1-mediated interactions and α4 integrin is redistributed onto the plasma membrane of primitive pyrenocytes. Both in vitro and in vivo studies indicate that fetal liver macrophage cells engulf primitive pyrenocytes. Taken together, these studies indicate that several aspects of the interplay between macrophage cells and maturing erythroid precursor cells are conserved during the ontogeny of mammalian organisms.

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pRB and E2F4 play distinct cell-intrinsic roles in fetal erythropoiesis

Cited by 12 publications

References 30 publications

Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages

Cytotoxicity of quantum dots and graphene oxide to erythroid cells and macrophages

Erythropoiesis and Megakaryopoiesis in a Dish

Interaction of the Macrophage and Primitive Erythroid Lineages in the Mammalian Embryo

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