Praziquantel against monogeneans of tambaqui (Colossoma macropomum)

Abstract

Praziquantel (PZQ) is an anthelminthic drug that has been tested worldwide to treat parasitosis in farmed fish. Information regarding the applicability and sustainability of medications in tambaqui Colossoma macropomum farming is highly needed. This study investigated the effects of PZQ at different concentrations and exposure times on the hematological parameters of tambaqui and the efficacy against monogeneans. The toxicity test with PZQ was conducted at three exposure times (0.5 h, 1 h and 24 h). In each of them, five PZQ concentrations (3.12, 6.25, 12.5, 25 and 50 mg L −1 ) were evaluated, with a control group, in triplicate, with six fish per unit. The same assays were conducted to evaluate the toxicity of ethanol, used as solvent. Based on the toxicity test and an in vitro test, the efficacy of PZQ against monogeneans in tambaqui was tested in four treatments (6.25, 12.5, 25 and 35 mg L −1 ), with long bath duration (24 h), with a control group, in triplicate, with six fish per unit. Fish were sampled and subjected to hematological and parasitological analyses. The use of ethanol as solvent did not interfere significantly with the physiology of tambaqui. In the toxicity test with PZQ, the main hematological alterations were those related to the time of exposure, with reduction in hematocrit, hemoglobin and MCHC, in addition to a significant dose and time-dependent alteration in blood glucose. No mortality was observed, except three fish exposed to 50 mg L −1 for 24 h. No treatment removed 100% of the parasites from the gills of tambaqui; however, the lowest concentrations of PZQ were more effective against Anacanthorus sp., removing 61.8% at 12.5 mg L −1 . With these results, it is possible to consider key moments during tambaqui farming to apply the medication to prevent disease outbreaks.

Efficacy of chlorine, sodium chloride and trichlorfon baths against monogenean Dawestrema cycloancistrium parasite of pirarucu Arapaima gigas

“…of parasites in treated group) x 100/mean no. of parasites in control group (Maciel & Affonso, 2021;Sommerville et al, 2016).…”

Optimal feeding rate and feeding frequency during the grow-out phase (95-350 g) of tambaqui (Colossoma macropomum) reared in earthen ponds

“…Plasma was collected by centrifuging the total blood at 1,500 × g for 10 min at 4°C. Total blood on day 84 was used for total hemogram, including: (a) hematocrit; (b) total red blood cell (RBC); (c) hemoglobin concentration; (d) mean corpuscular volume (MCV); (e) mean corpuscular hemoglobin concentration (MCHC), as described by Maciel and Affonso (2021). Plasma glucose, triglycerides, total protein, and cholesterol were evaluated on days 28, 56 and 84 using commercial kits (Labtest Diagnóstica S.A., Lagoa Santa, MG, Brazil) and spectrophotometric readings.…”

Acute toxicity of praziquantel to fish Danio rerio and planktonic crustacean Daphnia magna

“…In aquaculture, PZQ can be administered by bath or orally. Bath treatments are effective against monogeneans (Schmahl and Mehlhorn 1985;Morales-Serna et al 2018;Maciel and Affonso 2021), digeneans (Szekely and Molnar 1991;Zuskova et al 2018), and cestodes (Mitchell 2004;Mitchell and Darwish 2009). Oral treatments via intubation or in the feed are effective against monogeneans (Kim and Cho 2000;Forwood et al 2016), cestodes (Sudova et al 2010), blood flukes (Shirakashi et al 2012), and acanthocephalans (Zuskova et al 2018).…”