PRAF1: a Golgi complex transmembrane protein that interacts with virusesThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

Compton, Shannon; Behrend, Ellen N.

doi:10.1139/o06-176

Cited by 13 publications

(5 citation statements)

References 77 publications

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“…Sivars and colleagues [43] proposed a third function of PRA1 as a guanine nucleotide dissociation inhibitor (GDI) displacement factor (GDF) that aids in recycling Rabs during vesicular trafficking. An additional role for PRA1 in lipid transport, modulation of lipid homeostasis, and cell migration has been proposed based on proteomic analysis of PRA1 depleted nasopharyngeal carcinoma cells [44] and further supported by studies implicating PRA1 in the fusion of transport vesicles with the plasma membrane [45]. Finally, evidence supports a role for PRA1 in transport and assembly of viral proteins, although in some cases it may play an inhibitory role [45-49].…”

Section: Discussionmentioning

confidence: 99%

“…An additional role for PRA1 in lipid transport, modulation of lipid homeostasis, and cell migration has been proposed based on proteomic analysis of PRA1 depleted nasopharyngeal carcinoma cells [44] and further supported by studies implicating PRA1 in the fusion of transport vesicles with the plasma membrane [45]. Finally, evidence supports a role for PRA1 in transport and assembly of viral proteins, although in some cases it may play an inhibitory role [45-49]. These proposed functions for PRA1 are in no way mutually exclusive and in many cases are overlapping.…”

Section: Discussionmentioning

confidence: 99%

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A role for prenylated rab acceptor 1 in vertebrate photoreceptor development

et al. 2012

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BackgroundThe rd1 mouse retina is a well-studied model of retinal degeneration where rod photoreceptors undergo cell death beginning at postnatal day (P) 10 until P21. This period coincides with photoreceptor terminal differentiation in a normal retina. We have used the rd1 retina as a model to investigate early molecular defects in developing rod photoreceptors prior to the onset of degeneration.ResultsUsing a microarray approach, we performed gene profiling comparing rd1 and wild type (wt) retinas at four time points starting at P2, prior to any obvious biochemical or morphological differences, and concluding at P8, prior to the initiation of cell death. Of the 143 identified differentially expressed genes, we focused on Rab acceptor 1 (Rabac1), which codes for the protein Prenylated rab acceptor 1 (PRA1) and plays an important role in vesicular trafficking. Quantitative RT-PCR analysis confirmed reduced expression of PRA1 in rd1 retina at all time points examined. Immunohistochemical observation showed that PRA1-like immunoreactivity (LIR) co-localized with the cis-Golgi marker GM-130 in the photoreceptor as the Golgi translocated from the perikarya to the inner segment during photoreceptor differentiation in wt retinas. Diffuse PRA1-LIR, distinct from the Golgi marker, was seen in the distal inner segment of wt photoreceptors starting at P8. Both plexiform layers contained PRA1 positive punctae independent of GM-130 staining during postnatal development. In the inner retina, PRA1-LIR also colocalized with the Golgi marker in the perinuclear region of most cells. A similar pattern was seen in the rd1 mouse inner retina. However, punctate and significantly reduced PRA1-LIR was present throughout the developing rd1 inner segment, consistent with delayed photoreceptor development and abnormalities in Golgi sorting and vesicular trafficking.ConclusionsWe have identified genes that are differentially regulated in the rd1 retina at early time points, which may give insights into developmental defects that precede photoreceptor cell death. This is the first report of PRA1 expression in the retina. Our data support the hypothesis that PRA1 plays an important role in vesicular trafficking between the Golgi and cilia in differentiating and mature rod photoreceptors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development

et al. 2012

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“…RABAC is involved in the assembling of retro and rotavirus and in the inhibition of the herpes life cycle [62]. The under expression of this gene in infected compared to control spat suggests a failure to slow the progression of the lytic phases of the herpes virus cycle.…”

Section: Discussionmentioning

confidence: 99%

Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields

et al. 2013

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BackgroundMassive mortalities have been observed in France since 2008 on spat and juvenile Pacific oysters, Crassostrea gigas. A herpes virus called OsHV-1, easily detectable by PCR, has been implicated in the mortalities as demonstrated by the results of numerous field studies linking mortality with OsHV-1 prevalence. Moreover, experimental infections using viral particles have documented the pathogenicity of OsHV-1 but the physiological responses of host to pathogen are not well known.ResultsThe aim of this study was to understand mechanisms brought into play against the virus during infection in the field. A microarray assay has been developed for a major part of the oyster genome and used for studying the host transcriptome across mortality on field. Spat with and without detectable OsHV-1 infection presenting or not mortality respectively were compared by microarray during mortality episodes. In this study, a number of genes are regulated in the response to pathogen infection on field and seems to argue to an implication of the virus in the observed mortality. The result allowed establishment of a hypothetic scheme of the host cell’s infection by, and response to, the pathogen.ConclusionsThis response shows a “sensu stricto” innate immunity through genic regulation of the virus OsHV-1 life cycle, but also others biological processes resulting to complex interactions between host and pathogens in general.

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“…293 Nonetheless, studies have also shown an interaction of PRA1 with proteins from other viruses, including a lipid raft-associated rotavirus spike protein (VP4) 294,295 and the LMP1 oncoprotein of Epstein-Barr virus 296 (reviewed in Ref. 297 ).…”

Section: General Models For Hiv-1 Env Glycoprotein Incorporationmentioning

confidence: 99%

HIV-1 Envelope Glycoprotein Biosynthesis, Trafficking, and Incorporation

Checkley

Luttge

Freed

2011

Journal of Molecular Biology

402

445

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The HIV-1 envelope (Env) glycoproteins play an essential role in the virus replication cycle by mediating the fusion between viral and cellular membranes during the entry process. The Env glycoproteins are synthesized as a polyprotein precursor, gp160, that is cleaved by cellular proteases to the mature surface glycoprotein gp120 and the transmembrane glycoprotein gp41. During virus assembly the gp120/gp41 complex is incorporated as heterotrimeric spikes into the lipid bilayer of nascent virions. These gp120/gp41 complexes then initiate the infection process by binding receptor and co-receptor on the surface of target cells. Much is currently known about the HIV-1 Env glycoprotein trafficking pathway and the structure of gp120 and the extracellular domain of gp41. However, the mechanism by which the Env glycoprotein complex is incorporated into virus particles remains incompletely understood. Genetic data support a major role for the cytoplasmic tail of gp41 and the matrix domain of Gag in Env glycoprotein incorporation. Still to be defined are the identities of host cell factors that may promote Env incorporation, and the role of specific membrane microdomains in this process. Here we review our current understanding of HIV-1 Env glycoprotein trafficking and incorporation into virions.

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PRAF1: a Golgi complex transmembrane protein that interacts with virusesThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

Cited by 13 publications

References 77 publications

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development

Physiological change under OsHV-1 contamination in Pacific oyster Crassostrea gigas through massive mortality events on fields

HIV-1 Envelope Glycoprotein Biosynthesis, Trafficking, and Incorporation

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