A Quasi experimental design was used in the study. Sample size was 100 registered staff nurses with 50 in control group and 50 in experimental group working in intensive care units of selected hospitals of Jalandhar, Punjab. For sample collection, Convenience sampling technique was used. Descriptive and inferential statistics was used for data analysed. The result revealed that in experimental group there was significant difference between pre test and post test mean knowledge score & SD (10.72 ± 3.252) and (18.22 ± 3.893) respectively, whereas in control group there was no significant difference between pre test and post test mean knowledge score & SD (10.34 ± 2.344) and (10.44 ± 2.459) respectively regarding endotracheal suctioning among staff nurses. Furthermore, It was found that in experimental group there was a significant difference between pre test and post test mean practices score & SD (21.66±2.677) and (28.50±3.581) respectively, whereas in control group there was no significant difference between pre test and post test mean practices score & SD (21.30 ± 3.518) and (21.18 ± 3.433) respectively regarding endotracheal suctioning among staff nurses. Thus it was concluded that the structured teaching programme was effective in improving knowledge and practices regarding endotracheal suctioning among staff nurses in experimental group.