2013
DOI: 10.1159/000351814
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Practical Problems Encountered during the Cultivation of an Open-Source Reconstructed Human Epidermis Model on a Polycarbonate Membrane and Protein Quantification

Abstract: During recent years, the importance of in vitro technology in skin research has increased significantly. A variety of skin culture models have been developed and commercialized. In this respect, the availability of reconstructed human epidermis (RHE) equivalents represents a significant improvement compared to the use of monolayer cultures. However, when an in-house RHE model is being developed, researchers might encounter some difficulties during cultivation. The scope of this paper is to report our experienc… Show more

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Cited by 14 publications
(6 citation statements)
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References 47 publications
(71 reference statements)
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“…However, it should be emphasized that there was no keratinocyte growth factor (KGF) added to the EpiLife ® medium during the culture at the air‐liquid interface. The use of KGF was proposed by Lemper et al and is frequently used for the generation of reconstructed human epidermis .…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…However, it should be emphasized that there was no keratinocyte growth factor (KGF) added to the EpiLife ® medium during the culture at the air‐liquid interface. The use of KGF was proposed by Lemper et al and is frequently used for the generation of reconstructed human epidermis .…”
Section: Discussionmentioning
confidence: 99%
“…The condition of the keratinocytes in culture, however, appears to be highly dependent on the right source of media . For instance, Poumay et al used a specific culture medium (EpiLife ® ) for reconstruction of human epidermal models providing optimal conditions for growth and differentiation of keratinocytes . Given the fact that different cellular components such as keratinocytes or fibroblasts are likely to prefer distinct culture conditions for their optimal growth and differentiation, it remains unclear what type of media conditions are best suited for full‐thickness skin models combining distinct cellular components.…”
Section: Introductionmentioning
confidence: 99%
“…8,20 For the construction of RHE, hEK were isolated from infantile foreskin biopsies after receiving an informed consent and ethics approval granted by the Ethics Committee of the Landesärztekammer Baden-Württemberg (IGBZSF-2012-078). After cell expansion, the cells were seeded to cell culture inserts with a diameter of 0.59 cm and a pore size of 0.4 lm (Brand, Wertheim, Germany).…”
Section: Generation Of Reconstructed Human Epidermismentioning
confidence: 99%
“…In order to ensure appropriate and harmonized quality of the tissues produced by the new implementing laboratories, however, the technology transfer should ideally be organized through an experienced tissue engineering laboratory with proven know-how for 3D tissue model production. Some laboratories have reported practical problems with the development of an OS epidermal tissues, 27 clearly indicating the importance of organizing regular technical training sessions in-house or on-site. The same holds true for training on technical handling and on use of the OS-based 3D model.…”
Section: Availabilitymentioning
confidence: 99%