Practical implications of inconsistent germination and viability results in testing stored Fagus sylvatica seeds

Bezděčková, L.; Procházková, Z.; Matějka, K.

doi:10.12657/denbio.071.004

Cited by 3 publications

(2 citation statements)

References 10 publications

(11 reference statements)

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“…In the process of stratification of F. sylvatica seeds, the following indices of germination were determined: germination rate, mean germination time, mean daily germination, coefficient of speed germination (Bezděčková et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Quality changes during storage of beech seeds collected from different places

Elisovetcaia,

Ivanova,

Popovschi

et al. 2023

Plant Protection – Achievements and Perspectives

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In this study, the changes in quality of Fagus sylvatica seeds collected in 2020 from different places of the Republic of Moldova (Plaiul Fagului, Hîrjauca) and Ukraine (Zakarpattia) on the third year of storage were evaluated. Beech seeds quality maintenance depended on the values of moisture, initial viability and seeds germination. The seeds from the Plaiul Fagului with a higher initial germination 76.7-84.0% and lower moisture content (8.69-9.24%) slightly reduced their quality during storage, by 1.0-2.3%.The germination indices such as germination rate, mean daily germination, average seed germination time, total time of seed germination, were improved by treatment of stored seeds with the natural bioregulator, genistifolioside. Treatment with genistifolioside also contributed to the elongation of seed roots by 17-24% in all variants.

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Section: Methodsmentioning

confidence: 99%

Quality changes during storage of beech seeds collected from different places

Elisovetcaia,

Ivanova,

Popovschi

et al. 2023

Plant Protection – Achievements and Perspectives

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“…The changes of ABA content and fumarase activity in embryonic axes as well as all histological experiments were conducted using lot B7 and verified using lot S12 (both harvested in 2011 in Moravia, Czech Republic). Dormant seeds were analysed just after harvesting; moist stratification (without any growing medium) at a low temperature (3°C) was applied for 13-15 weeks (Bezděčková et al 2014). The seeds after stratification were not dormant, according to germination tests conducted at the Research Station Kunovice.…”

Section: Methodsmentioning

confidence: 99%

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Eliášová¹,

Pešek²,

Vondráková³

2015

Dendrobiology

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Understanding the mechanisms of seed dormancy is essential for planning optimal seed storage and for breaking the dormancy of stored seeds. Our experiments focused on three aspects of characterization of breaking the dormancy of Fagus sylvatica seeds during moist low temperature stratification. 1) We used the changes of ABA content in embryos during the whole process of stratification as the marker of the depth of dormancy: the decrease in the depth of dormancy (according standard germination tests) was directly proportional to the drop in endogenous ABA content in both cotyledons and embryonic axes. 2) Fumarase activity (the second marker of dormancy) increased gradually during stratification.3) The histochemical visualisation of storage compounds as the third marker of dormancy documented the changes in storage protein deposition during stratification. The proteinaceous content of storage vacuoles disappeared; the vacuoles diminished, petered out or fused, creating central vacuoles. This process commenced with imbibition of the seeds and it was more pronounced in the cells in the external parts of the cotyledons. No changes in the size and location of starch grains or calcium oxalate crystals linked with stratification were detected.

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