Practical aspects of preparation, optimization, and evaluation of poly(styrene-divinylbenzene)porous-layer open tubular (PLOT) columns for capillary liquid chromatography

Burato, Juliana Soares da Silva; Borsatto, João Victor Basolli; Lanças, Fernando Mauro

doi:10.1016/j.talanta.2022.124106

Cited by 2 publications

(2 citation statements)

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“…However, preparing a PLOT column with an i.d. smaller than 10 μm is challenging because blockings can be formed, especially in the polymerization step [108,109]. Despite these limitations, columns smaller than 10 μm i.d.…”

Section: Porous Layer Otmentioning

confidence: 99%

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Open tubular liquid chromatography: Recent advances and future trends

Medina

Cardoso

Borsatto

et al. 2023

J of Separation Science

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Nano‐liquid chromatography (nanoLC) is gaining significant attention as a primary analytical technique across various scientific domains. Unlike conventional high‐performance LC, nanoLC utilizes columns with inner diameters (i.ds.) usually ranging from 10 to 150 μm and operates at mobile phase flow rates between 10 and 1000 nl/min, offering improved chromatographic performance and detectability. Currently, most exploration of nanoLC has focused on particle‐packed columns. Although open tubular LC (OTLC) can provide superior performance, optimized OTLC columns require very narrow i.ds. (< 10 μm) and demand challenging instrumentation. At the moment, these challenges have limited the success of OTLC. Nevertheless, remarkable progress has been made in developing and utilizing OTLC systems featuring narrow columns (< 2 μm). Additionally, significant efforts have been made to explore larger columns (10–75 μm i.d), demonstrating practical applicability in many situations. Due to their perceived advantages, interest in OTLC has resurged in the last two decades. This review provides an updated outlook on the latest developments in OTLC, focusing on instrumental challenges, achievements, and advancements in column technology. Moreover, it outlines selected applications that illustrate the potential of OTLC for performing targeted and untargeted studies.

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Section: Porous Layer Otmentioning

confidence: 99%

“…The same column could be applied to GC separations. Furthermore, it is essential to highlight that, although theoretically, the smaller the column, the higher the performance, in some practical experimental conditions, using a wider column may be worth it [108].…”

Section: Porous Layer Otmentioning

confidence: 99%