Practical Aspects of Liquid Scintillation Counting

Kobayashi, Yutaka; Maudsley, David V.

doi:10.1002/9780470110355.ch3

Cited by 52 publications

(1 citation statement)

References 137 publications

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“…We also raised wild-type and pnc-1(pk9605) animals on media supplemented with NMN, the product of Nampt. NMN rescued gonad development of the mutants (not shown), but it also induced uv1 cell necrosis in wild type, probably because NMN is hydrolytically unstable (Kobayashi and Maudsley, 1969) and degrades into NAM during culture. To reduce the chance of hydrolysis, we directly injected 50 mM NMN into the body cavity of young animals.…”

Section: Nadmentioning

confidence: 99%

Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival inC. elegans

et al. 2009

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Nicotinamide adenine dinucleotide (NAD+) is a central molecule in cellular metabolism and an obligate co-substrate for NAD+-consuming enzymes, which regulate key biological processes such as longevity and stress responses. Although NAD+ biosynthesis has been intensely studied, little analysis has been done in developmental models. We have uncovered novel developmental roles for a nicotinamidase(PNC), the first enzyme in the NAD+ salvage pathway of invertebrates. Mutations in the Caenorhabditis elegans nicotinamidase PNC-1 cause developmental and functional defects in the reproductive system;the development of the gonad is delayed, four uterine cells die by necrosis and the mutant animals are egg-laying defective. The temporal delay in gonad development results from depletion of the salvage pathway product NAD+, whereas the uv1 cell necrosis and egg-laying defects result from accumulation of the substrate nicotinamide. Thus, regulation of both substrate and product level is key to the biological activity of PNC-1. We also find that diet probably affects the levels of these metabolites, as it affects phenotypes. Finally, we identified a secreted isoform of PNC-1 and confirmed its extracellular localization and functional activity in vivo. We demonstrate that nicotinamide phosphoribosyltransferase (Nampt), the equivalent enzyme in nicotinamide recycling to NAD+ in vertebrates,can functionally substitute for PNC-1. As Nampt is also secreted, we postulate an evolutionarily conserved extracellular role for NAD+biosynthetic enzymes during development and physiology.

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Section: Nadmentioning

confidence: 99%

Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival inC. elegans

et al. 2009

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Biotransformation of Isosorbide Dinitrate in Humans

Down¹,

Chasseaud²,

Grundy³

1974

Journal of Pharmaceutical Sciences

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The fate of dinobuton and dinoseb on growing apples

Hawkins¹,

Saggers²

1974

Pestic. Sci.

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After topical application of dinobuton or dinoseb to apple fruits approximately 75% and 72% respectively of the applied material was lost after 28 days. From apples treated with dinobuton the unchanged compound was the major component present up until 8 days but at later times the amounts of polar transformation products and water‐soluble compounds predominated. Only small amounts of dinoseb were found at all times. A similar pattern of results was obtained from apples treated with dinoseb. The maximum amounts of material absorbed through the fruit skin were 3% from dinobuton‐treated apples and 7% from dinoseb‐treated applies.

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Practical Aspects of Liquid Scintillation Counting

Cited by 52 publications

References 137 publications

Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival inC. elegans

Nicotinamidase modulation of NAD+ biosynthesis and nicotinamide levels separately affect reproductive development and cell survival inC. elegans

Biotransformation of Isosorbide Dinitrate in Humans

The fate of dinobuton and dinoseb on growing apples

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