Practical Applications in Immunohistochemistry: Evaluation of Rejection and Infection in Organ Transplantation

Troxell, Megan L.; Lanciault, Christian

doi:10.5858/arpa.2015-0275-cp

Cited by 25 publications

(10 citation statements)

References 155 publications

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“…Approaches to specimen collection from the lower respiratory tract. Lower respiratory tract specimens such as sputum, bronchoalveolar lavage/wash, and lung tissue may be considered in cases where the patient may be infected with an emerging pathogen (173,174) or is under intensive/critical care for pneumonia (175), in cases involving autopsy (176), or where molecular detection requires pathological evidence of invasive disease (e.g., ADV infection in lung specimens of lung transplant patients) (177). In severe illness due to influenza and emerging pathogens, upper respiratory (372)(373)(374).…”

Section: Appropriate Specimen Collection Is Critical For Virus Detectmentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

et al. 2018

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SUMMARYRespiratory viral infections are associated with a wide range of acute syndromes and infectious disease processes in children and adults worldwide. Many viruses are implicated in these infections, and these viruses are spread largely via respiratory means between humans but also occasionally from animals to humans. This article is an American Society for Microbiology (ASM)-sponsored Practical Guidance for Clinical Microbiology (PGCM) document identifying best practices for diagnosis and characterization of viruses that cause acute respiratory infections and replaces the most recent prior version of the ASM-sponsored Cumitech 21 document,Laboratory Diagnosis of Viral Respiratory Disease, published in 1986. The scope of the original document was quite broad, with an emphasis on clinical diagnosis of a wide variety of infectious agents and laboratory focus on antigen detection and viral culture. The new PGCM document is designed to be used by laboratorians in a wide variety of diagnostic and public health microbiology/virology laboratory settings worldwide. The article provides guidance to a rapidly changing field of diagnostics and outlines the epidemiology and clinical impact of acute respiratory viral infections, including preferred methods of specimen collection and current methods for diagnosis and characterization of viral pathogens causing acute respiratory tract infections. Compared to the case in 1986, molecular techniques are now the preferred diagnostic approaches for the detection of acute respiratory viruses, and they allow for automation, high-throughput workflows, and near-patient testing. These changes require quality assurance programs to prevent laboratory contamination as well as strong preanalytical screening approaches to utilize laboratory resources appropriately. Appropriate guidance from laboratorians to stakeholders will allow for appropriate specimen collection, as well as correct test ordering that will quickly identify highly transmissible emerging pathogens.

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Section: Appropriate Specimen Collection Is Critical For Virus Detectmentioning

confidence: 99%

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

et al. 2018

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“…Examples of using the ABC approach are common in American Journal of Physiology-Heart and Circulatory Physiology publications ( 23 , 31 , 38 , 53 ). There are a number of articles that have reviewed immunohistochemistry approaches ( 55 , 57 ).…”

Section: Immunohistochemistry Immunofluorescence and Immunocytochemmentioning

confidence: 99%

Guidelines for authors and reviewers on antibody use in physiology studies

Brooks

Lindsey

2018

American Journal of Physiology-Heart and Circulatory Physiology

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Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches.Listen to this article’s corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/.

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“…Subclasses have been added to the histologic diagnosis of TCMR to provide greater delineation: IA, IB, IIA, IIB, and III (with IIA, IIB, and III based on the assumption that all v‐lesions indicate TCMR) . As we consider molecular markers of graft injury and rejection, understanding how these newer technologies may complement Banff and support the complex interpretation of histopathology is important …”

Section: Introductionmentioning

confidence: 99%

High levels of dd-cfDNA identify patients with TCMR 1A and borderline allograft rejection at elevated risk of graft injury

Stites

Kumar

Olaitan

et al. 2020

American Journal of Transplantation

111

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The clinical importance of subclinical, early T cell–mediated rejection (Banff TCMR 1A and borderline lesions) remains unclear, due, in part to the fact that histologic lesions used to characterize early TCMR can be nonspecific. Donor‐derived cell‐free DNA (dd‐cfDNA) is an important molecular marker of active graft injury. Over a study period from June 2017 to May 2019, we assessed clinical outcomes in 79 patients diagnosed with TCMR 1A/borderline rejection across 11 US centers with a simultaneous measurement of dd‐cfDNA. Forty‐two patients had elevated dd‐cfDNA (≥0.5%) and 37 patients had low levels (<0.5%). Elevated levels of dd‐cfDNA predicted adverse clinical outcomes: among patients with elevated cfDNA, estimated glomerular filtration rate declined by 8.5% (interquartile rate [IQR] −16.22% to −1.39%) (−3.50 mL/min/1.73 m2 IQR −8.00 to −1.00) vs 0% (−4.92%, 4.76%) in low dd‐cfDNA patients (P = .004), de novo donor‐specific antibody formation was seen in 40% (17/42) vs 2.7% (P < .0001), and future or persistent rejection occurred in 9 of 42 patients (21.4%) vs 0% (P = .003). The use of dd‐cfDNA may complement the Banff classification and to risk stratify patients with borderline/TCMR 1A identified on biopsy.

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Practical Applications in Immunohistochemistry: Evaluation of Rejection and Infection in Organ Transplantation

Cited by 25 publications

References 155 publications

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections

Guidelines for authors and reviewers on antibody use in physiology studies

High levels of dd-cfDNA identify patients with TCMR 1A and borderline allograft rejection at elevated risk of graft injury

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