2011
DOI: 10.1002/bio.1200
|View full text |Cite
|
Sign up to set email alerts
|

Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin–luciferase bioluminescence

Abstract: Firefly luciferin-luciferase bioluminescence is known for its high quantum yield (41.0 ± 7.4%). Given this high quantum yield, application of this bioluminescence is expected to be useful in the field of clinical diagnostics. The kinetic profile of this bioluminescence exhibits an instant rise (<1 s) and a rapid decay in light emission (decreased to 42% after 5 s). In this study, we applied four enhancers including coenzyme A, inosine5'-triphosphate sodium salt, sodium tripolyphosphate and potassium pyrophosph… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

0
12
0

Year Published

2012
2012
2024
2024

Publication Types

Select...
7
1

Relationship

2
6

Authors

Journals

citations
Cited by 13 publications
(12 citation statements)
references
References 13 publications
0
12
0
Order By: Relevance
“…An enzyme-labeled anti-HBs rabbit polyclonal antibody (I.L. Japan, Tokyo, Japan) and an anti-HBs MAb, HBs335, were prepared using a previously described method (36,37). In brief, through the reaction with maleimide-activated streptavidin (MP Biomedicals, Solon, OH), the Fab= fragments conjugated with thermostable biotinylated luciferase (bL248; Kikkoman, Chiba, Japan) were purified using Superdex 200 HR 10/30 (GE Healthcare Japan, Tokyo, Japan) and then by affinity absorption again to remove nonspecifically bound materials, using a gel coated with normal human serum (37).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…An enzyme-labeled anti-HBs rabbit polyclonal antibody (I.L. Japan, Tokyo, Japan) and an anti-HBs MAb, HBs335, were prepared using a previously described method (36,37). In brief, through the reaction with maleimide-activated streptavidin (MP Biomedicals, Solon, OH), the Fab= fragments conjugated with thermostable biotinylated luciferase (bL248; Kikkoman, Chiba, Japan) were purified using Superdex 200 HR 10/30 (GE Healthcare Japan, Tokyo, Japan) and then by affinity absorption again to remove nonspecifically bound materials, using a gel coated with normal human serum (37).…”
Section: Methodsmentioning
confidence: 99%
“…Antibodies with streptavidin and thermostable biotinylated luciferase bind through an avidinbiotin interaction, allowing for the conjugation of luciferase on an antibody without decreasing the reactivity of luciferase. Some measurement systems using luciferase as a labeling enzyme have already been reported (36)(37)(38). In a previous study, we developed a measurement system for HBsAg with a high sensitivity of 10 mIU/ml on the basis of a bioluminescent enzyme immunoassay (BLEIA) using firefly luciferase with a high quantum yield (36,37).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…The emission of visible light through by the two‐step oxidation of firefly luciferin, in the presence of ATP, Mg 2+ , and molecular oxygen has prepared the susceptibility of accomplishment of ATP‐related assays , analytical assays and in vivo molecular imaging . Meanwhile, luciferase can act as a molecular probe for protein‐protein interactions and different analytes , and as well as a label for immunoassays .…”
Section: Introductionmentioning
confidence: 99%
“…This makes them a widely used tool in a variety of in vitro and in vivo applications: in ATP-related assays from direct ATP measurements to estimation of bacterial contamination and pyrosequencing [4, 5], in in vivo molecular imaging and as a genetic reporter in molecular biology [68]. This enzyme was also shown to be a promising tool for molecular sensing of protein-protein interactions and different analytes [911], in analytical assays based on real time monitoring of polynucleotide amplification [12] and a label for immunoassays [13]. …”
Section: Introductionmentioning
confidence: 99%