Pqs<scp>R</scp>‐dependent and Pqs<scp>R</scp>‐independent regulation of motility and biofilm formation by <scp>PQS</scp> in <i>Pseudomonas aeruginosa</i> PAO1

Qiao, Guo; Kong, Weina; Jin, Sheng; Chen, Lin; Xu, Yangyang; Duan, Kangmin

doi:10.1002/jobm.201300091

Cited by 41 publications

(45 citation statements)

References 58 publications

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“…In contrast, a significant decrease in expression of the quorum sensing rhlI and pqsA genes was detected in response to 2‐TDC (Fig. C), which could explain the negative effect of the MK on P. aeruginosa biofilms since it has been observed that these two QS systems stimulate biofilm formation (Duan and Surette, ; Guo et al ., ) and are required for biofilm maturation (Rampioni et al ., ).…”

Section: Resultsmentioning

confidence: 97%

2‐Tridecanone impacts surface‐associated bacterial behaviours and hinders plant–bacteria interactions

López‐Lara

Nogales

Pech-Canul

et al. 2018

Environmental Microbiology

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Surface motility and biofilm formation are behaviours which enable bacteria to infect their hosts and are controlled by different chemical signals. In the plant symbiotic alpha-proteobacterium Sinorhizobium meliloti, the lack of long-chain fatty acyl-coenzyme A synthetase activity (FadD) leads to increased surface motility, defects in biofilm development and impaired root colonization. In this study, analyses of lipid extracts and volatiles revealed that a fadD mutant accumulates 2-tridecanone (2-TDC), a methylketone (MK) known as a natural insecticide. Application of pure 2-TDC to the wild-type strain phenocopies the free-living and symbiotic behaviours of the fadD mutant. Structural features of the MK determine its ability to promote S. meliloti surface translocation, which is mainly mediated by a flagella-independent motility. Transcriptomic analyses showed that 2-TDC induces differential expression of iron uptake, redox and stress-related genes. Interestingly, this MK also influences surface motility and impairs biofilm formation in plant and animal pathogenic bacteria. Moreover, 2-TDC not only hampers alfalfa nodulation but also the development of tomato bacterial speck disease. This work assigns a new role to 2-TDC as an infochemical that affects important bacterial traits and hampers plant-bacteria interactions by interfering with microbial colonization of plant tissues.

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Section: Resultsmentioning

confidence: 97%

2‐Tridecanone impacts surface‐associated bacterial behaviours and hinders plant–bacteria interactions

López‐Lara

Nogales

Pech-Canul

et al. 2018

Environmental Microbiology

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“…The repression of swarming motility when ReaL is overexpressed is here shown to be completely pqsR independent. We suggest that, even in the absence of autoinduction (i.e., in a pqsR ‐ background), HHQ/PQS levels might reach values that repress swarming motility through unknown PqsR independent mechanisms similar to those described for PAO1 strain (Guo et al ., ). However, the regulation by ReaL of functions that inversely regulate biofilm formation and swarming motility, such as the diguanylate cyclase SadC (Merritt et al ., ) and phosphodiesterase BifA (Kuchma et al ., ) cannot be ruled out.…”

Section: Discussionmentioning

confidence: 97%

The small RNA ReaL: a novel regulatory element embedded in the Pseudomonas aeruginosa quorum sensing networks

Carloni

Macchi

Sattin

et al. 2017

Environmental Microbiology

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The small RNA ReaL of the opportunistic pathogen Pseudomonas aeruginosa has been characterized. Our results indicate that ReaL contributes to P. aeruginosa virulence. In the Galleria mellonella infection model, reaL gene deletion resulted in decreased virulence, while ReaL overexpression resulted in a hyper-virulent phenotype. We also demonstrate that ReaL is embedded in the P. aeruginosa quorum sensing (QS) with the role of linking las to pqs systems. We show that ReaL is negatively regulated by the las regulator LasR and impacts positively the synthesis of the pqs quinolone signal PQS by a positive post-transcriptional effect on the pqsC gene. Perturbations of ReaL levels affect pyocyanin synthesis, biofilm formation and swarming motility, processes that are known to be influenced by PQS synthesis. In addition to being regulated by LasR, ReaL is also responding to infection relevant cues that P. aeruginosa can experience in mammalian hosts such as temperature and oxygen availability. Furthermore, ReaL shows a growth phase-dependent pattern of expression, being up-regulated in stationary phase, due to the activity of the alternative σ factor RpoS. Together, these regulations of ReaL expression are expected to contribute to the fine co-modulation of PQS synthesis and, ultimately, virulence.

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“…Unbound dye in the wells were washed off by deionized water before 200 μ L of 95% ethanol was added to dissolve the crystal violet stains. The absorbance of the solutions was then measured at 570 nm [7, 22]. …”

Section: Methodsmentioning

confidence: 99%

“…P. aeruginosa cells in biofilms are surrounded by exopolysaccharides and form a structured aggregates, and these cells exhibit increased resistance to antibiotics and other adversary agents [3–6]. Infections caused by biofilm-forming P. aeruginosa, such as those in cystic fibrosis lung, are almost impossible to eradicate [7]. There is an urgent need to find novel antimicrobial agents to control such infections [8, 9].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Pseudomonas aeruginosa Biofilm Formation by Traditional Chinese Medicinal Herb Herba patriniae

Dang

et al. 2017

BioMed Research International

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New antimicrobial agents are urgently needed to treat infections caused by drug-resistant pathogens and by pathogens capable of persisting in biofilms. The aim of this study was to identify traditional Chinese herbs that could inhibit biofilm formation of Pseudomonas aeruginosa, an important human pathogen that causes serious and difficult-to-treat infections in humans. A luxCDABE-based reporter system was constructed to monitor the expression of six key biofilm-associated genes in P. aeruginosa. The reporters were used to screen a library of 36 herb extracts for inhibitory properties against these genes. The results obtained indicated that the extract of Herba patriniae displayed significant inhibitory effect on almost all of these biofilm-associated genes. Quantitative analysis showed that H. patriniae extract was able to significantly reduce the biofilm formation and dramatically altered the structure of the mature biofilms of P. aeruginosa. Further studies showed H. patriniae extract decreased exopolysaccharide production by P. aeruginosa and promoted its swarming motility, two features disparately associated with biofilm formation. These results provided a potential mechanism for the use of H. patriniae to treat bacterial infections by traditional Chinese medicines and revealed a promising candidate for exploration of new drugs against P. aeruginosa biofilm-associated infections.

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PqsR‐dependent and PqsR‐independent regulation of motility and biofilm formation by PQS in Pseudomonas aeruginosa PAO1

Cited by 41 publications

References 58 publications

2‐Tridecanone impacts surface‐associated bacterial behaviours and hinders plant–bacteria interactions

2‐Tridecanone impacts surface‐associated bacterial behaviours and hinders plant–bacteria interactions

The small RNA ReaL: a novel regulatory element embedded in the Pseudomonas aeruginosa quorum sensing networks

Inhibition of Pseudomonas aeruginosa Biofilm Formation by Traditional Chinese Medicinal Herb Herba patriniae

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