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Poulson, Mary E.; Edwards, Gerald E.; Browse, John

doi:10.1023/a:1016054027464

Cited by 17 publications

(4 citation statements)

References 26 publications

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“…However, more is known about TFAs as being the precursors of JA. Indeed, the first Arabidopsis mutant lacking TFAs, the fad3 fad7 fad8 mutant, was male sterile (6), susceptible to pathogens and insects (12,31), and susceptible to environmental stress (7,8,32). In most of these cases, the application of exogenous JA or MeJA was sufficient to restore plant fertility or to protect significantly from stress, therefore confirming the critical function of TFAs as the precursors of JA.…”

Section: Discussionmentioning

confidence: 91%

“…Instead, TFAs in leaves have roles in environmental stress and in defense responses. At low temperatures, TFA depletion in Arabidopsis both compromises recovery from photoinhibition (7) and also impairs the process of chloroplast maintenance (8). In bacterial pathogenesis, the translocation of ALA from the chloroplast to extrachloroplastic membranes may help activate NADPH oxidase, an enzyme responsible for the production of reactive oxygen species necessary for full resistance to avirulent pathogens (9).…”

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confidence: 99%

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Nonenzymatic Oxidation of Trienoic Fatty Acids Contributes to Reactive Oxygen Species Management in Arabidopsis

Mène-Saffrané¹,

Dubugnon

Chételat

et al. 2009

Journal of Biological Chemistry

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In higher plants such as Arabidopsis thaliana, omega-3 trienoic fatty acids (TFAs), represented mainly by ␣-linolenic acid, serve as precursors of jasmonic acid (JA), a potent lipid signal molecule essential for defense. The JA-independent roles of TFAs were investigated by comparing the TFA-and JA-deficient fatty acid desaturase triple mutant (fad3-2 fad7-2 fad8 (fad3 fad7 fad8)) with the aos (allene oxide synthase) mutant that contains TFAs but is JA-deficient. When challenged with the fungus Botrytis, resistance of the fad3 fad7 fad8 mutant was reduced when compared with the aos mutant, suggesting that TFAs play a role in cell survival independently of being the precursors of JA. An independent genetic approach using the lesion mimic mutant accelerated cell death2 (acd2-2) confirmed the importance of TFAs in containing lesion spread, which was increased in the lines in which the fad3 fad7 fad8 and acd2-2 mutations were combined when compared with the aos acd2-2 lines. Malondialdehyde, found to result from oxidative TFA fragmentation during lesion formation, was measured by gas chromatography-mass spectrometry. Its levels correlated with the survival of the tissue. Furthermore, plants lacking TFAs overproduced salicylic acid (SA), hydrogen peroxide, and transcripts encoding several SA-regulated and SA biosynthetic proteins. The data suggest a physiological role for TFAs as sinks for reactive oxygen species.

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Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

Nonenzymatic Oxidation of Trienoic Fatty Acids Contributes to Reactive Oxygen Species Management in Arabidopsis

Mène-Saffrané¹,

Dubugnon

Chételat

et al. 2009

Journal of Biological Chemistry

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“…The net carbon assimilation rate gained from these measurements is in the range of previously published rates for Arabidopsis. In the literature, measurements for whole Arabidopsis plants grown under a 12 h light/12 h dark cycle range between 5 and 10 µmol m −2 s −1 [2, 9, 13]. The standard deviation of 0.34 is relatively low compared to the value of ~0.6 observed for the gas exchange systems used by Sun et al [2] and Tocquin et al [10].…”

Section: Resultsmentioning

confidence: 99%

A whole-plant chamber system for parallel gas exchange measurements of Arabidopsis and other herbaceous species

Kölling

George

Künzli³

et al. 2015

Plant Methods

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BackgroundPhotosynthetic assimilation of carbon is a defining feature of the plant kingdom. The fixation of large amounts of carbon dioxide supports the synthesis of carbohydrates, which make up the bulk of plant biomass. Exact measurements of carbon assimilation rates are therefore crucial due to their impact on the plants metabolism, growth and reproductive success. Commercially available single-leaf cuvettes allow the detailed analysis of many photosynthetic parameters, including gas exchange, of a selected leaf area. However, these cuvettes can be difficult to use with small herbaceous plants such as Arabidopsis thaliana or plants having delicate or textured leaves. Furthermore, data from single leaves can be difficult to scale-up for a plant shoot with a complex architecture and tissues in different physiological states. Therefore, we constructed a versatile system—EGES-1—to simultaneously measure gas exchange in the whole shoots of multiple individual plants. Our system was designed to be able record data continuously over several days.ResultsThe EGES-1 system yielded comparable measurements for eight plants for up to 6 days in stable, physiologically realistic conditions. The chambers seals have negligible permeability to carbon dioxide and the system is designed so as to detect any bulk-flow air leaks. We show that the system can be used to monitor plant responses to changing environmental conditions, such as changes in illumination or stress treatments, and to compare plants with phenotypically severe mutations. By incorporating interchangeable lids, the system could be used to measure photosynthetic gas exchange in several genera such as Arabidopsis, Nicotiana, Pisum, Lotus and Mesembryanthemum.ConclusionEGES-1 can be introduced into a variety of growth facilities and measure gas exchange in the shoots diverse plant species grown in different growth media. It is ideal for comparing photosynthetic carbon assimilation of wild-type and mutant plants and/or plants undergoing selected experimental treatments. The system can deliver valuable data for whole-plant growth studies and help understanding mutant phenotypes. Overall, the EGES-1 is complementary to the readily-available single leaf systems that focus more on the photosynthetic process in within the leaf lamina.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-015-0089-z) contains supplementary material, which is available to authorized users.

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“…The BIN of lipid metabolism includes genes related to lipid degradation, sphingolipids, fatty acid (FA) synthesis, FA elongation and phospholipid synthesis ( Fig 6 ). In a study with Arabidopsis, polyunsaturated FAs played an important role in responses of photosynthesis and stomatal conductance to environmental stresses such as high VPD [ 38 ]. Similarly, changes in gene expression related to lipid metabolism were observed in Arabidopsis due to drought [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean

2015

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Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

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Untitled

Cited by 17 publications

References 26 publications

Nonenzymatic Oxidation of Trienoic Fatty Acids Contributes to Reactive Oxygen Species Management in Arabidopsis

Nonenzymatic Oxidation of Trienoic Fatty Acids Contributes to Reactive Oxygen Species Management in Arabidopsis

A whole-plant chamber system for parallel gas exchange measurements of Arabidopsis and other herbaceous species

Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean

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