“…Cells were routinely grown at 28 30°C with orbital agitation (220 rpm) in 750 ml flasks in 100 150 ml of liq uid modified Rider's medium, pH 4.5 4.6, containing (g/liter): MgSO 4 ·7H 2 O, 0.5; NaCl, 0.1; CaCl 2 ·2H 2 O, 0.05; KH 2 PO 4 , 8.6; KNO 3 , 0.3; glycerol, 7.5; Na 2 WO 4 ·2H 2 O, 0.33, supplemented with microelements and vitamins as described earlier [16]. In the control experiments, Na 2 WO 4 ·2H 2 O was omitted and the medi um contained only trace amounts of Mo as impurities from the reagents used.…”