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Alcalá, Pilar; Ferrer-Miralles, Neus; Feliu, Jordi X.; Villaverde, Antonio

doi:10.1023/a:1020371220100

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…scFv93 is a recombinant version of the SD6 antibody, which binds specifically to a main B‐cell epitope from foot‐and‐mouth disease virus (FMDV) from serotype C 1 , sited in the VP1 capsid protein [13]. The scFv93‐encoding DNA was generated by PCR amplification (driven by the proofreading Deep Vent ® DNA polymerase) from plasmid pCASD6 93 [14], using two alternative pairs of complementary oligonucleotides. The pair CSD6S (5′‐GGG ATC CCA GGT GAA ACT GCA GC‐3′) and CSD6R (5′‐GGG ATC CCC GTT TTA TTT CCA GC‐3′) was used to obtain a product for ligation into the Eco RI‐digested vector pJLACZ, resulting in vector pPAlacZCSD6.…”

Section: Methodsmentioning

confidence: 99%

Engineering of Escherichia coli β‐galactosidase for solvent display of a functional scFv antibody fragment

2002

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Protein engineering allows the generation of hybrid polypeptides with functional domains from di¡erent origins and therefore exhibiting new biological properties. We have explored several permissive sites in Escherichia coli L L-galactosidase to generate functional hybrid enzymes displaying a mouse scFv antibody fragment. When this segment was placed at the amino-terminus of the enzyme, the whole fusion protein was stable, maintained its speci¢c activity and interacted speci¢cally with the target antigen, a main antigenic determinant of foot-andmouth disease virus. In addition, the antigen-targeted enzyme was enzymatically active when bound to the antigen and therefore useful as a reagent in single-step immunoassays. These results prove the £exibility of E. coli L L-galactosidase as a carrier for large-sized functional domains with binding properties and prompt the further exploration of the biotechnological applicability of the scFv enzyme targeting principle for diagnosis or other biomedical applications involving antigen tagging.

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Section: Methodsmentioning

confidence: 99%

Engineering of Escherichia coli β‐galactosidase for solvent display of a functional scFv antibody fragment

2002

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“…(Vo-Dinh, et al 2000;Thé venot et al 2001;Mohanty 2001). Concerning the biological specificity the biosensors could be developed like, Antibody/antigen based, Enzymes based (Alcala et al 2002); Nucleic acids based (Kwakye and Baeumner 2003); cellular structures/cells based (Kitova, et al 2002;Köhler, et. al 2000;Dubey and Upadhyay 2001;Degrassi et.…”

Section: A) In Medicinementioning

confidence: 99%

Nanotechnology and Global Applications: Bench to Community

Chakraborty

Guha

Patra

2020

JBLS

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Advances in nanotechnology has become enormously promising in the field of health science, in developing many medical equipment. The possible effects of novel nanomedical technology significantly improve the diagnosis and therapeutic aspects of many diseases. Nanomaterials are being applied in operation, disease diagnosis as well as therapy, molecular imaging, implant technology, tissue engineering, as well as a device for efficient drug delivery, protein and gene release. In this review we discussed the utility of nanoscience in health issues, and the knowledge from there how can be disseminated to the educators and parents for expanding STEM (Science, Technology, Engineering and Math) programs.

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“…They made up of a biological factor responsible for sampling, as well as a physical element (often called transducer) transmitting sampling outcome for moreover processing [5][6][7][8]. The biological element of a biosensor contains a biosensitive layer, which can either contain bioreceptors or be made of bioreceptors covalently attached to the transducer.Concerning the biological specificity conferring mechanism used one can distinguish 5 major categories of biosensors 1) Antibody/antigen based, 2) Enzymes based (mono-or multi enzyme systems) [9], 3) Nucleic acids based (DNA, cDNA, RNA) [10] 4) Based on cellular interactions (cellular structures/cells),utilisating the whole cells (microorganisms, such asbacteria, fungi, eukaryotic cells or yeast) or cell organellesor particles (mitochondria, cell walls, tissue slices) [11][12][13][14][15][16] 5) Employing biomimetic materials (e.g., synthetic bioreceptors). Biosensors based on nucleic acid interactions of biological element are called DNA biosensors, or genosensors, or biodetectors.…”

Section: Biosensorsmentioning

confidence: 99%

The Application of Nanotechnology in Medical Sciences: New Horizon of Treatment

Moshed¹,

Sarkar²,

Khaleque³

2017

Am. J. Biomed. Sci.

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Nanotechnology has become enormously promising technology during last few decades and now it is estimated to have a significant effect on medical equipment. The possible effects of novel nanomedical applications on sickness identification, treatment, as well as anticipation are expected to improve healthiness care greatly. In addition, therapeutic selection may gradually be adapted to all patients' profile. Applications of nanomaterials are reported in operation, cancer diagnosis as well as therapy, biodetection of sickness markers, molecular imaging, implant technology, tissue engineering, as well as devices for drug, protein, and gene and radionuclide release. Various medical nanotechnology applications are at a standstill in their immaturity. A larger amount of nanodevices are presently under medical examination as well as several devices are commercially available. In this review work some applications of nanodevices in medical technology are also summarized.

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Untitled

Cited by 11 publications

References 23 publications

Engineering of Escherichia coli β‐galactosidase for solvent display of a functional scFv antibody fragment

Engineering of Escherichia coli β‐galactosidase for solvent display of a functional scFv antibody fragment

Nanotechnology and Global Applications: Bench to Community

The Application of Nanotechnology in Medical Sciences: New Horizon of Treatment

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