PPTGAL, a convenient Gal4 P‐element vector for testing expression of enhancer fragments in <i>drosophila</i>

Sharma, Yashoda; Cheung, Una S.; Larsen, Ellen; Eberl, Daniel F.

doi:10.1002/gene.10127

Cited by 117 publications

(110 citation statements)

References 12 publications

(16 reference statements)

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“…DNA immediately 5′ of the DmCCAP coding region and extending from -516 to +39 bp was obtained by PCR using wild-type genomic DNA as a template. The PCR products were inserted into the pPTGAL transformation vector (Sharma et al, 2002). The recombinant DmCCAP promoter-GAL4 fusion (CCAP-GAL4) construct was introduced into the germline using standard methods.…”

Section: Generation Of Ccap-gal4 Drivermentioning

confidence: 99%

Targeted ablation of CCAP neuropeptide-containing neurons ofDrosophilacauses specific defects in execution and circadian timing of ecdysis behavior

et al. 2003

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Insect growth and metamorphosis is punctuated by molts, during which a new cuticle is produced. Every molt culminates in ecdysis, the shedding of the remains of the old cuticle. Both the timing of ecdysis relative to the molt and the actual execution of this vital insect behavior are under peptidergic neuronal control. Based on studies in the moth, Manduca sexta, it has been postulated that the neuropeptide Crustacean cardioactive peptide (CCAP)plays a key role in the initiation of the ecdysis motor program. We have used Drosophila bearing targeted ablations of CCAP neurons (CCAP KO animals) to investigate the role of CCAP in the execution and circadian regulation of ecdysis. CCAP KO animals showed specific defects at ecdysis, yet the severity and nature of the defects varied at different developmental stages. The majority of CCAP KO animals died at the pupal stage from the failure of pupal ecdysis, whereas larval ecdysis and adult eclosion behaviors showed only subtle defects. Interestingly, the most severe failure seen at eclosion appeared to be in a function required for abdominal inflation, which could be cardioactive in nature. Although CCAP KO populations exhibited circadian eclosion rhythms, the daily distribution of eclosion events (i.e.,gating) was abnormal. Effects on the execution of ecdysis and its circadian regulation indicate that CCAP is a key regulator of the behavior. Nevertheless, an unexpected finding of this work is that the primary functions of CCAP as well as its importance in the control of ecdysis behaviors may change during the postembryonic development of Drosophila.

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Section: Generation Of Ccap-gal4 Drivermentioning

confidence: 99%

Targeted ablation of CCAP neuropeptide-containing neurons ofDrosophilacauses specific defects in execution and circadian timing of ecdysis behavior

et al. 2003

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“…We also cloned the same cis-regulatory region into a new, highly versatile GAL4 transformation vector (pPT-GAL4; Sharma et al, 2002). For this cloning, a KpnI to NotI fragment was excised from P[w ϩ ; dpp85.8MX.lacZ] and inserted into these sites in pPTGAL4 to create P[w ϩ ; PS.GAL4].…”

mentioning

confidence: 99%

Posterior spiracle specific GAL4 lines: New reagents for developmental biology and respiratory physiology

Takaesu

Johnson

Newfeld

2002

Genesis

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“…tslGAL4 constructs. To generate the tsl(G)GAL4 construct, an EcoRI/BamHI fragment from C4PLZ-tsl(G) was subcloned into the pPTGAL vector (35). To generate the tsl(F)GAL4 construct, an EcoRI/BamHI fragment from C4PLZ-tsl(F) was subcloned into the pChs-Gal4 vector (36).…”

Section: Methodsmentioning

confidence: 99%

Two distinct but convergent groups of cells trigger Torso receptor tyrosine kinase activation by independently expressing torso-like

Furriols

Ventura

Casanova

2007

Proc. Natl. Acad. Sci. U.S.A.

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PPTGAL, a convenient Gal4 P‐element vector for testing expression of enhancer fragments in drosophila

Cited by 117 publications

References 12 publications

Targeted ablation of CCAP neuropeptide-containing neurons ofDrosophilacauses specific defects in execution and circadian timing of ecdysis behavior

Targeted ablation of CCAP neuropeptide-containing neurons ofDrosophilacauses specific defects in execution and circadian timing of ecdysis behavior

Posterior spiracle specific GAL4 lines: New reagents for developmental biology and respiratory physiology

Two distinct but convergent groups of cells trigger Torso receptor tyrosine kinase activation by independently expressing torso-like

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