PpAtg30 Tags Peroxisomes for Turnover by Selective Autophagy

Farré, Jean‐Claude; Manjithaya, Ravi; Mathewson, Richard D.; Subramani, Suresh

doi:10.1016/j.devcel.2007.12.011

Cited by 299 publications

(384 citation statements)

References 31 publications

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“…Recently, Atg30 has been described to be the autophagy receptor for peroxisome turnover in Pichia pastoris (Farré et al, 2008). This protein is found localized on the peroxisomal membrane via its interaction with two peroxisomal membrane proteins, Pex14p and Pex3p.…”

Section: Discussionmentioning

confidence: 99%

NBR1 acts as an autophagy receptor for peroxisomes

Deosaran

Larsen

Hua

et al. 2012

Journal of Cell Science

290

359

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SummarySelective macro-autophagy is an intracellular process by which large cytoplasmic materials are selectively sequestered and degraded in the lysosomes. Substrate selection is mediated by ubiquitylation and recruitment of ubiquitin-binding autophagic receptors such as p62, NBR1, NDP52 and Optineurin. Although it has been shown that these receptors act cooperatively to target some types of substrates to nascent autophagosomes, their precise roles are not well understood. We examined selective autophagic degradation of peroxisomes (pexophagy), and found that NBR1 is necessary and sufficient for pexophagy. Mutagenesis studies of NBR1 showed that the amphipathic a-helical J domain, the ubiquitin-associated (UBA) domain, the LC3-interacting region and the coiled-coil domain are necessary to mediate pexophagy. Strikingly, substrate selectivity is partly achieved by NBR1 itself by coincident binding of the J and UBA domains to peroxisomes. Although p62 is not required when NBR1 is in excess, its binding to NBR1 increases the efficiency of NBR1-mediated pexophagy. Together, these results suggest that NBR1 is the specific autophagy receptor for pexophagy.

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Section: Discussionmentioning

confidence: 99%

NBR1 acts as an autophagy receptor for peroxisomes

Deosaran

Larsen

Hua

et al. 2012

Journal of Cell Science

290

359

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“…Saccharomyces cerevisiae, respectively 36,37 . In mammalian cells, pexophagy requires the autophagy cargo receptors p62 and NBR1, which recruit LC3-positive phagophores to monoubiquitylated peroxisomes 38,39 .…”

Section: Glomerular Podocytesmentioning

confidence: 99%

Autophagy at the crossroads of catabolism and anabolism

Kaur

Debnath

2015

Nat Rev Mol Cell Biol

826

801

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“…Atg36 and PpAtg30 are receptors for pexophagy in S. cerevisiae and P. pastoris, respectively [143,144]. In both yeast and mammalian cells Pex3 and Pex14 function as cargo ligands [145,146].…”

Section: Pexophagymentioning

confidence: 99%

The machinery of macroautophagy

et al. 2013

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Autophagy is a primarily degradative pathway that takes place in all eukaryotic cells. It is used for recycling cytoplasm to generate macromolecular building blocks and energy under stress conditions, to remove superfluous and damaged organelles to adapt to changing nutrient conditions and to maintain cellular homeostasis. In addition, autophagy plays a critical role in cytoprotection by preventing the accumulation of toxic proteins and through its action in various aspects of immunity including the elimination of invasive microbes and its participation in antigen presentation. The most prevalent form of autophagy is macroautophagy, and during this process, the cell forms a double-membrane sequestering compartment termed the phagophore, which matures into an autophagosome. Following delivery to the vacuole or lysosome, the cargo is degraded and the resulting macromolecules are released back into the cytosol for reuse. The past two decades have resulted in a tremendous increase with regard to the molecular studies of autophagy being carried out in yeast and other eukaryotes. Part of the surge in interest in this topic is due to the connection of autophagy with a wide range of human pathophysiologies including cancer, myopathies, diabetes and neurodegenerative disease. However, there are still many aspects of autophagy that remain unclear, including the process of phagophore formation, the regulatory mechanisms that control its induction and the function of most of the autophagy-related proteins. In this review, we focus on macroautophagy, briefly describing the discovery of this process in mammalian cells, discussing the current views concerning the donor membrane that forms the phagophore, and characterizing the autophagy machinery including the available structural information.

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PpAtg30 Tags Peroxisomes for Turnover by Selective Autophagy

Cited by 299 publications

References 31 publications

NBR1 acts as an autophagy receptor for peroxisomes

NBR1 acts as an autophagy receptor for peroxisomes

Autophagy at the crossroads of catabolism and anabolism

The machinery of macroautophagy

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