PPARγ Promotes Mannose Receptor Gene Expression in Murine Macrophages and Contributes to the Induction of This Receptor by IL-13

Coste, Agnès; Dubourdeau, Marc; Linas, Marie Denise; Cassaing, Sophie; Lepert, Jean-Claude; Balard, Patricia; Chalmeton, Sandrine; Bernad, José; Orfila, C; Séguéla, J. P.; Pipy, Bernard

doi:10.1016/s1074-7613(03)00229-2

Cited by 81 publications

(93 citation statements)

References 38 publications

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“…Recently we have shown, in murine peritoneal Mu, that IL-13 upregulates mannose receptor expression via PPARc activation. We have also shown that this induction of the mannose receptor by IL-13 was dependent on cytosolic PLA2 activation [23].These two results demonstrating PPARc involvement in IL-13-induced mannose receptor expression or in IL-4 induced CD36 expression were obtained using murine Mu. The extrapolation of these data between different species and between different stages of Mu differentiation remains difficult.…”

supporting

confidence: 67%

Section: Discussionmentioning

confidence: 66%

“…The cells were transfected with a mouse PPARc expression vector (pCMX-mPPARc) or a beetle b-galactosidase expression vector as control as previously described [23]. Cells were collected 24 h later and analyzed as described in Analysis of CD36 expression using flow cytometry.…”

Section: Transfection Assaysmentioning

confidence: 99%

“…Firstly, IL-4 up-regulates monocyte/Mu CD36 by the generation of an arachidonic acid metabolite that is a PPARc agonist [18], and we have shown that mouse Mu stimulated by IL-13 are also able to produce these metabolites [22]. Additionally, we have recently demonstrated with resident peritoneal Mu in mice that IL-13 activates PPARc via the endogenous production of 15d-PGJ 2 , an arachidonic acid metabolite, and induces the expression of mannose receptors [23].In this study we demonstrate that IL-13 induces CD36 expression on human blood monocytes via a PPARc-dependent signaling pathway involving activation of cytosolic phospholipase A2 (PLA2). …”

mentioning

confidence: 91%

“…cDNA was synthesized using the SuperScript System (Invitrogen, Carisbad, CA), random hexamer primers and dNTP. Quantitative RT-PCR was performed on a LightCycler as described previously [23]. The 18S rRNA transcript was used as the invariant control.…”

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IL‐13 induces expression of CD36 in human monocytes through PPARγ activation

et al. 2007

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The class B scavenger receptor CD36 is a component of the pattern recognition receptors on monocytes that recognizes a variety of molecules. CD36 expression in monocytes depends on exposure to soluble mediators. We demonstrate here that CD36 expression is induced in human monocytes following exposure to IL-13, a Th2 cytokine, via the peroxisome proliferator-activated receptor (PPAR)c pathway. Induction of CD36 protein was paralleled by an increase in CD36 mRNA. The PPARc pathway was demonstrated using transfection of a PPARc expression plasmid into the murine macrophage cell line RAW264.7, expressing very low levels of PPARc, and in peritoneal macrophages from PPARc-conditional null mice. We also show that CD36 induction by IL-13 via PPARc is dependent on phospholipase A2 activation and that IL-13 induces the production of endogenous 15-deoxy-D 12,14 -prostaglandin J 2 , an endogenous PPARc ligand, and its nuclear localization in human monocytes. Finally, we demonstrate that CD36 and PPARc are involved in IL-13-mediated phagocytosis of Plasmodium falciparum-parasitized erythrocytes. These results reveal a novel role for PPARc in the alternative activation of monocytes by IL-13, suggesting that endogenous PPARc ligands, produced by phospholipase A2 activation, could contribute to the biochemical and cellular functions of CD36.Leukocyte signaling * These 2 authors contributed equally to this work. IntroductionThe innate immune system protects the host in the early phase of infection. Circulating monocytes and tissue macrophages (Mu) mediate much of this innate immune response [1]. The strategy of recognition in the innate response is mediated by the coordinated action of pathogen-associated molecular patterns and pattern recognition receptors. Scavenger receptors and mannose receptor are important pattern recognition receptors in monocytes/Mu [2,3]. The modulation of the expression of these receptors may be critical in the role of these cells in antigen processing, scavenging, and host defence against pathogens. Several members of the scavenger receptor family, including Mu class A scavenger receptors and CD36, have been identified as receptors for modified lipoproteins on Mu, and their relevance to lipid uptake has been demonstrated in vitro and in vivo [4]. The scavenger receptor CD36, an 88-kDa integral membrane protein, is a class B scavenger receptor expressed on a wide variety of cells, in particular on monocytes and monocytederived Mu [5,6]. CD36 is known as a receptor for the uptake of oxidatively modified low-density lipoprotein (LDL) [7] and is also able to bind anionic phospholipid phosphatidylserine [8]. This scavenger receptor is implicated in the clearance of apoptotic cells [9]. Recently, McGilvray and colleagues [10] described a CD36-dependent nonopsonic phagocytosis of erythrocytes containing P. falciparum, by monocytes and culture-derived Mu, and a decrease in the parasiteinduced TNF secretion by monocytes/Mu. These processes were accentuated by CD36 up-regulation by peroxisome proliferator-acti...

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supporting

confidence: 67%

Section: Discussionmentioning

confidence: 66%

Section: Transfection Assaysmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 99%

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IL‐13 induces expression of CD36 in human monocytes through PPARγ activation

et al. 2007

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CCN family 2/connective tissue growth factor (CCN2/CTGF) promotes osteoclastogenesis via induction of and interaction with dendritic cell–specific transmembrane protein (DC-STAMP)

Nishida

Emura

Kubota

et al. 2010

Journal of Bone and Mineral Research

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CCN family 2/connective tissue growth factor (CCN2/CTGF) promotes endochondral ossification. However, the role of CCN2 in the replacement of hypertrophic cartilage with bone is still unclear. The phenotype of Ccn2 null mice, having an expanded hypertrophic zone, indicates that the resorption of the cartilage extracellular matrix is impaired therein. Therefore, we analyzed the role of CCN2 in osteoclastogenesis because cartilage extracellular matrix is resorbed mainly by osteoclasts during endochondral ossification. Expression of the Ccn2 gene was upregulated in mouse macrophage cell line RAW264.7 on day 6 after treatment of glutathione S transferase (GST) fusion mouse receptor activator of NF-κB ligand (GST-RANKL), and a combination of recombinant CCN2 (rCCN2) and GST-RANKL significantly enhanced tartrate-resistant acid phosphatase (TRACP)–positive multinucleated cell formation compared with GST-RANKL alone. Therefore, we suspected the involvement of CCN2 in cell-cell fusion during osteoclastogenesis. To clarify the mechanism, we performed real-time PCR analysis of gene expression, coimmunoprecipitation analysis, and solid-phase binding assay of CCN2 and dendritic cell–specific transmembrane protein (DC-STAMP), which is involved in cell-cell fusion. The results showed that CCN2 induced and interacted with DC-STAMP. Furthermore, GST-RANKL–induced osteoclastogenesis was impaired in fetal liver cells from Ccn2 null mice, and the impaired osteoclast formation was rescued by the addition of exogenous rCCN2 or the forced expression of DC-STAMP by a retroviral vector. These results suggest that CCN2 expressed during osteoclastogenesis promotes osteoclast formation via induction of and interaction with DC-STAMP.

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Innate and Adaptive Immunity to Systemic Candida albicans Infection

Romani

2005

Fungal Immunology

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PPARγ Promotes Mannose Receptor Gene Expression in Murine Macrophages and Contributes to the Induction of This Receptor by IL-13

Cited by 81 publications

References 38 publications

IL‐13 induces expression of CD36 in human monocytes through PPARγ activation

IL‐13 induces expression of CD36 in human monocytes through PPARγ activation

CCN family 2/connective tissue growth factor (CCN2/CTGF) promotes osteoclastogenesis via induction of and interaction with dendritic cell–specific transmembrane protein (DC-STAMP)

Innate and Adaptive Immunity to Systemic Candida albicans Infection

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