PPARγ-Mediated p21 Induction in Aerodigestive Preneoplastic Cell Lines

Miller, Wendy A.; Wuertz, Beverly R.; Ondrey, Frank G.

doi:10.1177/0003489418787833

Cited by 5 publications

(5 citation statements)

References 55 publications

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“…To our knowledge, this is the first epidemiological study using population‐based case‐control methodology to assess the association between pioglitazone use among patients with type 2 DM and the subsequent occurrence of HNC. Our findings contradict previous laboratory research findings, two phase II clinical trials, and a prospective cohort study, which suggested a chemoprotective effect of pioglitazone in oral/head and neck cancers. Notably our study not only showed no protective effect but, on the contrary, shows increased odds of oral cavity and HNC cancer with pioglitazone use.…”

Section: Discussioncontrasting

confidence: 94%

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Association between pioglitazone use and head and neck cancer: Population‐based case‐control study

Yang

Xirasagar

et al. 2019

Head & Neck

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Background This study aimed to evaluate the association between pioglitazone use and the occurrence of head and neck cancer. Methods Data for this case‐control study were retrieved from the Taiwan National Health Insurance Research Database. A total of 21 464 diabetic patients newly diagnosed with head and neck cancers were identified. We used propensity score matching to select 64 392 comparison patients (3:1 ratio). Multiple logistic regression modeling was used to examine the association of head and neck cancer with pioglitazone use in the 5 years preceding the cancer diagnosis. Results Bivariate analysis showed a significant difference in the prevalence of prior using pioglitazone between cases and controls (19.3% vs 18.5%, P < .001) was observed. Multiple regression analysis showed adjusted odds of pioglitazone use of 1.06 (95% CI: 1.02‐1.10) among cases relative to controls. Conclusions Prior pioglitazone use was associated with oral cavity cancer.

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Section: Discussioncontrasting

confidence: 94%

“…PPARγ agonists as a chemoprevention agent for HNC have been established in many laboratory studies . There remains a paucity of human clinical studies, specifically whether PPARγ agonists such as pioglitazone used in patients with type 2 DM could be preventive against HNC .…”

Section: Introductionmentioning

confidence: 99%

Association between pioglitazone use and head and neck cancer: Population‐based case‐control study

Yang

Xirasagar

et al. 2019

Head & Neck

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“…On the other hand, Miller et al demonstrated that the induction of p21 is rapid and does not last long. In their study, the strongest increase in p21 protein and transcript levels was observed after 6 h of incubation with PPAR-γ activator; however, after 24 h, this effect diminished [51]. As our shortest incubation period with CLA was 24 h, it is possible that we could omit any possible changes in CDKN1A mRNA level.…”

Section: Cell Line/genementioning

confidence: 80%

The influence of conjugated linoleic acid on the expression of peroxisome proliferator-activated receptor-γ and selected apoptotic genes in non-small cell lung cancer

et al. 2020

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In recent years, peroxisome proliferator-activated receptor-γ (PPARγ) has been intensively studied. Because its activation is often associated with changes in the expression level of various apoptotic genes, many studies have emphasized the role of PPARγ as an important anticancer agent. However, in different types of cancer, different genes are influenced by PPARγ action. Previous studies showed that conjugated linoleic acid (CLA) was able to induce apoptosis, upregulate PPARG gene expression and activate PPARγ protein in certain human cancer cell lines. Moreover, some PPARγ agonists inhibited the growth of human lung cancer cells through the induction of apoptosis. Nevertheless, the impact of CLA on PPARγ mRNA and protein levels in non-small cell lung cancer (NSCLC) cell lines has not been investigated thus far. Therefore, in our study, we analysed the influence of the c9,t11 linoleic acid isomer on the expression of PPARG and other genes involved in the apoptotic response (BCL-2, BAX, and CDKN1A) in two NSCLC cell lines of different histological origin (A549 and Calu-1) and in normal human bronchial epithelial Beas-2B cells. Cells were treated with several doses of c9,t11 CLA, followed by RNA and protein isolation, cDNA synthesis, real-time quantitative PCR (RT-qPCR) and Western blot analysis. We showed that the investigated CLA isomer was able to enhance the expression of PPARγ in the examined cell lines and alter the mRNA and protein levels of genes involved in apoptosis. Fluorescent staining and MMT assay revealed the antiproliferative potential of CLA as well as its ability to activate pathways that lead to cell death.

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“…Despite limited studies that have has been conducted on the PPARs, they have been linked to various conditions such as the levels of lipoprotein, cardiovascular disease, diabetes, obesity, and malignancies [27]. PPARs exposed an active role in glucose and lipid metabolism, and the regular functioning of the adipose cells [28].…”

Section: Discussionmentioning

confidence: 99%

Expressions of Nuclear Factor-kappa B and Peroxisome Proliferator-activated Receptor-Gamma Proportional with Clinical Staging of Nasopharyngeal Carcinoma

Farhat

Rahmi²,

Chrestella³

et al. 2021

Open Access Maced J Med Sci

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BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignancy induced by the mutation of the transcription factors nuclear factor-kappa B (NF-kB) and peroxisome proliferator-activated receptor-gamma (PPAR-gamma). There was no known of the study about the association and targeted therapy of NF-kB and PPAR-gamma-induced NPC. AIM: This study analyzed and compared the proportion of NF-kB and PPAR-gamma and its association with the clinical characteristic of various NPC patients. METHODS: This was a cross-sectional study and conducted in Adam Malik General Hospital. The samples were paraffin block tissue obtained from 58 NPC patients and underwent immunohistochemistry staining for NF-kB or PPAR-gamma overexpression in March–November 2018. Determination of overexpression was based on the immunoreactive score. The association of NF-kB or PPAR-gamma overexpression with the clinical characteristics of the patients was analyzed using Fisher’s exact test. RESULTS: This study showed a significant increase of NF-kB and PPAR-gamma (p < 0.05). Male was found common than women (3.46:1) with non-keratinizing squamous cell carcinoma as the most common form of NPC (75.9%) and the 41–60 years old is the most common age (56.9%). Overexpression of NF-kB and PPAR-gamma was found mostly in T3-T4 (66.0%; 69.6%), N+ (92.5%; 91.3%), and clinical Stage IV (67.9%; 73.9%), respectively. CONCLUSION: The number of samples overexpressed was proportional to the clinical stage of NPC. This study provides an insight into the relationship of NF-kB and PPAR-gamma to NPC, suggesting their role in the development of malignancy.

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PPARγ-Mediated p21 Induction in Aerodigestive Preneoplastic Cell Lines

Cited by 5 publications

References 55 publications

Association between pioglitazone use and head and neck cancer: Population‐based case‐control study

Association between pioglitazone use and head and neck cancer: Population‐based case‐control study

The influence of conjugated linoleic acid on the expression of peroxisome proliferator-activated receptor-γ and selected apoptotic genes in non-small cell lung cancer

Expressions of Nuclear Factor-kappa B and Peroxisome Proliferator-activated Receptor-Gamma Proportional with Clinical Staging of Nasopharyngeal Carcinoma

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