PPARγ and GLUT-4 expression as developmental regulators/markers for preadipocyte differentiation into an adipocyte

Fernyhough, Melinda E.; Okine, E. K.; Hausman, Gary; Vierck, Janet L.; Dodson, Michael V.

doi:10.1016/j.domaniend.2007.05.001

Cited by 116 publications

(108 citation statements)

References 120 publications

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“…Studies have shown that the FABP4 gene is located within the confidence interval of some QTL detected in the region, which are involved in the regulation of serum leptin levels in mice (Ogino et al, 2003). Several studies have associated leptin with sexual precocity (Foster and Nagatani, 1999;Moore et al, 2003;Wu et al, 2005;Fernyhough et al, 2007). Interestingly, however, the leptin gene was included in the present study and we found no significant effect on the trait analyzed.…”

Section: Discussioncontrasting

confidence: 54%

Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle

et al. 2015

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ABSTRACT. The objective of this study was to identify associations between known polymorphisms in genes related to adipose tissue and sexual precocity in Nellore cattle. A total of 1689 precocious and nonprecocious heifers belonging to farms participating in Conexão Delta G breeding program were studied. SNPs from the Illumina High-Density Bovine SNP BeadChip were used. This chip contains 777,000 SNPs located within the region of the candidate genes at a distance of up to 5 kb, considering that linkage disequilibrium (LD) exists at this distance. Linear models were used for statistical analysis. The fastPHASE and GenomeStudio programs were used for haplotype reconstruction and LD analysis based on r 2 statistics. Fifty-seven candidate genes and 443 SNPs were analyzed: among the latter, 370 SNPs formed 83 haplotypes, while the remaining SNPs were studied separately. Statistical analysis showed that only three haplotypes, one haplotype consisting of two SNPs located in the FABP4 gene and two haplotypes consisting of four and Lipid metabolism-related genes as candidates of precocity two SNPs located in the PPP3CA gene, had a significant effect on sexual precocity at P < 0.05. It can be concluded that the FABP4 and PPP3CA genes influence sexual precocity and may therefore be used in selection programs designed to improve sexual precocity in Nellore cattle.

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Section: Discussioncontrasting

confidence: 54%

Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle

et al. 2015

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“…24 We found that stevioside treatment improved adipogenesis and glucose uptake in visceral adipose tissue, evidenced by higher expressions of Lxra, Fabp4 and Glut4. 25,26 The Glut4 27 and Fabp4 28 are direct transcriptional targets for the LXR/retinoid X-receptor heterodimer. Thus, the induction of Lxra in adipose tissue of stevioside-treated mice supports the increased expression of Glut4, which favors glucose uptake, and of Fabp4, which improves fatty acid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Stevioside inhibits atherosclerosis by improving insulin signaling and antioxidant defense in obese insulin-resistant mice

et al. 2009

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Objective: Stevioside is a non-caloric natural sweetener that does not induce a glycemic response, making it attractive as sweetener to diabetics and others on carbohydrate-controlled diets. Obesity is frequently associated with insulin resistance and increased inflammation and oxidative stress. Therefore, we investigated its effects on insulin resistance, inflammation and oxidative stress related to atherosclerosis in obese insulin-resistant mice.Research design: Twelve-week-old mice were treated with stevioside (10 mg kg À1 , n ¼ 14) or placebo (n ¼ 20) for 12 weeks. Results: Stevioside had no effect on weight and triglycerides, but lowered glucose and insulin. Stevioside treatment improved adipose tissue maturation, and increased glucose transport, insulin signaling and antioxidant defense in white visceral adipose tissues. Together, these increases were associated with a twofold increase of adiponectin. In addition, stevioside reduced plaque volume in the aortic arch by decreasing the macrophage, lipid and oxidized low-density lipoprotein (ox-LDL) content of the plaque. The higher smooth muscle cell-to-macrophage ratio was indicative for a more stable plaque phenotype. The decrease in ox-LDL in the plaque was likely due to an increase in the antioxidant defense in the vascular wall, as evidenced by increased Sod1, Sod2 and Sod3. Circulating adiponectin was associated with improved insulin signaling and antioxidant defense in both the adipose tissue and the aorta of stevioside-treated mice. Conclusion: Stevioside treatment was associated with improved insulin signaling and antioxidant defense in both the adipose tissue and the vascular wall, leading to inhibition of atherosclerotic plaque development and inducing plaque stabilization.

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“…However, adipose tissue formation in beef cattle is a complicated biological process associated with the genetic background, development, and nutrition of an animal, maintained by unique molecular signaling pathways [1-3]. Gene expression analyses, using a novel in vitro model of cattle adipocytes [4-6], showed that genes for peroxisome proliferator-activated receptors (PPARγ), CCAAT-enhancer binding proteins (C/EBPα, C/EBPβ) and sterol regulatory element binding protein (SREBP 1c) are directly or indirectly involved in the regulation of bovine adipogenesis [7-9]. In addition, the Wdnm1-like protein, a distant member of the whey acidic protein/four-disulfide core family, was shown to be associated with adipogenesis in livestock species as a remodeler of the extracellular milieu in adipogenesis and/or as a differentiation-dependent gene in white and brown adipogenesis [10].…”

Section: Introductionmentioning

confidence: 99%

Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues

et al. 2010

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BackgroundThe fat components of red meat products have been of interest to researchers due to the health aspects of excess fat consumption by humans. We hypothesized that differences in protein expression have an impact on adipose tissue formation during beef cattle development and growth. Therefore, in this study we evaluated the differences in the discernable proteome of subcutaneous adipose tissues of 35 beef crossbred steers [Charolais × Red Angus (CHAR) (n = 13) and Hereford × Angus (HEAN) (n = 22)] with different back fat (BF) thicknesses. The goal was to identify specific protein markers that could be associated with adipose tissue formation in beef cows.ResultsApproximately 541-580 protein spots were detected and compared in each crossbred group, and 33 and 36 protein spots showed expression differences between tissues with high and low BF thicknesses from HEAN and CHAR crossbed, respectively. The annexin 1 protein was highly expressed in both crossbred steers that had a higher BF thickness (p < 0.05) and this was further validated by a western blot analysis. In 13 tissues of CHAR animals and 22 tissues of HEAN animals, the relative expression of annexin 1 was significantly different (p < 0.05) between tissues with high and low BF thicknesses.ConclusionThe increased expression of annexin 1 protein has been found to be associated with higher BF thickness in both crossbred steers. This result lays the foundation for future studies to develop the protein marker for assessing animals with different BF thickness.

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PPARγ and GLUT-4 expression as developmental regulators/markers for preadipocyte differentiation into an adipocyte

Cited by 116 publications

References 120 publications

Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle

Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle

Stevioside inhibits atherosclerosis by improving insulin signaling and antioxidant defense in obese insulin-resistant mice

Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues

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