PPAR γ Protects Against Hypoxic Renal Tubular Epithelial Cell Injury in Rat Model: A Randomized Controlled Trial

Abstract

Background To explore t function of peroxisome proliferator-activated receptor γ(PPARγ) in renal tissue in acute hypoxic renal rat model injury. Methods 24 male SD rats were randomly divided into normal control group, PPARγ agonist group (rosiglitazone 10 mg/kg.d), PPARγ inhibitor group༈GW9662,1mg/kg·d༉and hypoxia injury group, with six rats in each group. The normal control group without any treatment, the other three groups were exposed to 7500 m altitude for seven hours of acute hypobaric hypoxia. The mRNA and protein expressions of PPARγ, superoxide dismutase (SOD), interleukin-1 β༈IL-1β༉ and renal endothelin(ET-1) in renal tissue were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and western blotting. The kidney’s morphology was observed by light microscope and electron microscope. Results The mRNA and protein expression levels of PPARγ and SOD in hypoxia injury group decreased significantly (P < 0.05), while the mRNA and protein expression levels of IL-1 β and ET-1 increased significantly compared with the normal control group (P < 0.05). After intervention with PPAR agonists, the PPARγ and SOD were elevated significantly, while IL-1 β and ET-1 were decreased significantly compared to the hypoxia injury group. The renal tubule epithelial cells (RTEC) were less damaged and abscission was reduced. Conclusions PPARγ protect renal tubular epithelial cells from hypoxia-induced injury. PPARγ agonists can be used as potential target interventions to alleviate acute hypoxic kidney injury.