PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer

Zhao, Liang; Zheng, Xiuli; Liu, Jingfang; Zheng, Rong; Yang, Rui; Wang, Ying; Sun, Lifang

doi:10.1016/j.biopha.2019.109251

Cited by 13 publications

(7 citation statements)

References 53 publications

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“…We performed the KEGG enrichment analysis of genes in the stage-specific modules for further functional categorization. The greenyellow module enriched pathways ‘Primary bile acid biosynthesis’ and ‘PPAR signaling pathway’ have been observed in human studies as regulating early placental development, physiology of clinical pregnancy, and prenatal complications [ 32 , 33 ]. The primary bile acid biosynthesis pathway and the PPAR signaling pathway in the embryo reflects their importance in regulating lipid metabolism and energy homeostasis during development [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti

et al. 2023

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Background The behaviors and ontogeny of Aedes aegypti are closely related to the spread of diseases caused by dengue (DENV), chikungunya (CHIKV), Zika (ZIKV), and yellow fever (YFV) viruses. During the life cycle, Ae. aegypti undergoes drastic morphological, metabolic, and functional changes triggered by gene regulation and other molecular mechanisms. Some essential regulatory factors that regulate insect ontogeny have been revealed in other species, but their roles are still poorly investigated in the mosquito. Results Our study identified 6 gene modules and their intramodular hub genes that were highly associated with the ontogeny of Ae. aegypti in the constructed network. Those modules were found to be enriched in functional roles related to cuticle development, ATP generation, digestion, immunity, pupation control, lectins, and spermatogenesis. Additionally, digestion-related pathways were activated in the larvae and adult females but suppressed in the pupae. The integrated protein‒protein network also identified cilium-related genes. In addition, we verified that the 6 intramodular hub genes encoding proteins such as EcKinase regulating larval molt were only expressed in the larval stage. Quantitative RT‒PCR of the intramodular hub genes gave similar results as the RNA-Seq expression profile, and most hub genes were ontogeny-specifically expressed. Conclusions The constructed gene coexpression network provides a useful resource for network-based data mining to identify candidate genes for functional studies. Ultimately, these findings will be key in identifying potential molecular targets for disease control.

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Section: Resultsmentioning

confidence: 99%

Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti

et al. 2023

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“…TGF-beta type 2 receptor (TGFBR2), the key regulator of TGF-beta signaling, was downregulated in both AKD and BKD TE. Peroxisome proliferator-activated receptor (PPAR) signaling regulates the important trophoblast functions such as immune tolerance, invasion, syncytialization, and metabolism [ 58 , 59 ]. According to our results, 5 downregulated genes in AKD TE, and 8 downregulated and 7 upregulated genes in BKD TE are involved in PPAR signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development

Ali

Iqbal

Abbas³

et al. 2022

Cells

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The proper conceptus elongation in ruminants is critical for the successful placentation and establishment of pregnancy. We have previously shown that the trophectoderm-specific knockdown of LIN28A/B in day 9 ovine blastocysts resulted in increased let-7 miRNAs and reduced conceptus elongation at day 16 of gestation. In this current study, by transcriptome analysis of LIN28A knockdown (AKD) or LIN28B knockdown (BKD) trophectoderm (TE), we explored the downstream target genes of the LIN28-let-7 axis and their roles in the placental and fetal development. We identified 449 differentially expressed genes (DEGs) in AKD TE and 1214 DEGs in BKD TE compared to non-targeting control (NTC). Our analysis further revealed that 210 downregulated genes in AKD TE and 562 downregulated genes in BKD TE were the potential targets of let-7 miRNAs. Moreover, 16 downregulated genes in AKD TE and 57 downregulated and 7 upregulated genes in BKD TE were transcription factors. The DEGs in AKD and BKD TE showed enrichment in the biological processes and pathways critical for placental development and function, and fetal development and growth. The results of this study suggest the potential roles of the LIN28-let-7 axis in placental and fetal development beyond its involvement in trophoblast proliferation and conceptus elongation.

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“…The PPAR signaling pathway, a critical pathway in lipid metabolism, regulates the expression of many genes involved in glucose and lipid homeostasis, inflammation, proliferation, and differentiation ( 49 – 51 ). Studies have shown that it may be an important predictor of breast cancer and regulated placental lipid metabolism ( 50 , 52 , 53 ). In chickens, some studies have also indicated that enrichment of the PPAR signaling pathway helps in adaptation during adverse conditions such as heat stress, and is required for energy metabolism and regulating the oxidative stress-induced inflammatory response ( 2 , 51 , 54 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Effects of Folic Acid Supplement on Gene Expression in Liver of Broiler Chickens

Zhang

Liu

et al. 2021

Front. Vet. Sci.

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Folic acid is a water-soluble B vitamin, and plays an important role in regulating gene expression and methylation. The liver is the major site of lipid biosynthesis in the chicken. Nevertheless, how gene expression and regulatory networks are affected by folic acid in liver of broilers are poorly understood. This paper conducted the RNA-seq technology on the liver of broilers under folic acid challenge investigation. First, 405 differentially expressed genes (DEGs), including 157 significantly upregulated and 248 downregulated, were detected between the control group (C) and the 5 mg folic acid group (M). Second, 68 upregulated DEGs and 142 downregulated DEGs were determined between C group and 10 mg folic acid group (H). Third, there were 165 upregulated genes and 179 downregulated genes between M and H groups. Of these DEGs, 903 DEGs were successfully annotated in the public databases. The functional classification based on GO and KEEGG showed that “general function prediction only” represented the largest functional classes, “cell cycle” (C vs. M; M vs. H), and “neuroactive ligand-receptor interaction” (C vs. H) were the highest unique sequences among three groups. SNP analysis indicated that numbers of C, M and H groups were 145,450, 146,131, and 123,004, respectively. Total new predicted alternative splicing events in C, M, and H groups were 9,521, 9,328, and 8,929, respectively. A protein-protein interaction (PPI) network was constructed, and the top 10 hub genes were evaluated among three groups. The results of real time PCR indicated that mRNA abundance of PPARγ and FAS in abdominal fat of M and H groups were reduced compared with the C group (P < 0.05). Ultramicroscopy results showed that folic acid could reduce lipid droplets in livers from chickens. Finally, contents of LPL, PPARγ, and FAS in abdominal fat were decreased with the folic acid supplmented diets (P < 0.01). These findings reveal the effects of folic acid supplemention on gene expression in liver of broilers, which can provide information for understanding the molecular mechanisms of folic acid regulating liver lipid metabolism.

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PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer

Cited by 13 publications

References 53 publications

Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti

Gene coexpression network during ontogeny in the yellow fever mosquito, Aedes aegypti

Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development

Transcriptome Analysis of Effects of Folic Acid Supplement on Gene Expression in Liver of Broiler Chickens

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