PPA-GCN: A Efficient GCN Framework for Prokaryotic Pathways Assignment

Abstract

With the rapid development of sequencing technology, completed genomes of microbes have explosively emerged. For a newly sequenced prokaryotic genome, gene functional annotation and metabolism pathway assignment are important foundations for all subsequent research work. However, the assignment rate for gene metabolism pathways is lower than 48% on the whole. It is even lower for newly sequenced prokaryotic genomes, which has become a bottleneck for subsequent research. Thus, the development of a high-precision metabolic pathway assignment framework is urgently needed. Here, we developed PPA-GCN, a prokaryotic pathways assignment framework based on graph convolutional network, to assist functional pathway assignments using KEGG information and genomic characteristics. In the framework, genomic gene synteny information was used to construct a network, and ideas of self-supervised learning were inspired to enhance the framework’s learning ability. Our framework is applicable to the genera of microbe with sufficient whole genome sequences. To evaluate the assignment rate, genomes from three different genera (Flavobacterium (65 genomes) and Pseudomonas (100 genomes), Staphylococcus (500 genomes)) were used. The initial functional pathway assignment rate of the three test genera were 27.7% (Flavobacterium), 49.5% (Pseudomonas) and 30.1% (Staphylococcus). PPA-GCN achieved excellence performance of 84.8% (Flavobacterium), 77.0% (Pseudomonas) and 71.0% (Staphylococcus) for assignment rate. At the same time, PPA-GCN was proved to have strong fault tolerance. The framework provides novel insights into assignment for metabolism pathways and is likely to inform future deep learning applications for interpreting functional annotations and extends to all prokaryotic genera with sufficient genomes.

A Novel Hierarchical Network-Based Approach to Unveil the Complexity of Functional Microbial Genome

“…Sequence similarity among all genes within the Microcystis genome was accessed using BLAST. To e ciently and rigorously identify similar genes, this study employed a bidirectional best hits approach, aggregating sequences that exhibited both a score and coverage rate surpassing 80% into a single node [35].…”

A Novel Hierarchical Network-Based Approach to Unveil the Complexity of Functional Microbial Genome

“…Node construction: Sequence similarity among all genes within the Microcystis genome was accessed using BLAST. To efficiently and rigorously identify similar genes, this study employed a bidirectional best hits approach, aggregating sequences that exhibited both a score and coverage rate surpassing 80% into a single node [35].…”