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Ajibade, S. R.; Weeden, N. F.; Chite, Stephen

doi:10.1023/a:1003763328768

Cited by 119 publications

(13 citation statements)

References 12 publications

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“…However, less number of ISSRs found more informative over 20 RAPD primers. The ability of ISSRs in detecting high polymorphism compared to RAPD is well known and observed by a number of studies [26,27]. Despite of the simplicity and rapidity of RAPD and ISSRs, a few technical disadvantages associated with these markers have decreased their fidelity as genetic markers and prevented their widespread use in recent years.…”

Section: Discussionmentioning

confidence: 99%

A set of SCAR markers in cluster bean (Cyamopsis tetragonoloba L. Taub) genotypes

Sharma¹,

Kumar²,

Raman³

et al. 2014

ABB

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Cluster bean, one of the most important cash legume crop has played an increasingly important role in wide range of industries. Owing to the significance of molecular marker studies in numerous applications including in genetic improvement of crops, there is an obvious need to undertake such studies in cluster bean. In the present work, 35 genotypes of cluster bean were collected from different states of India and analyzed using RAPD and ISSR markers. Further SCAR marker system was introduced in order to increase the reproducibility of the polymorphism and specificity. For this polymorphic (RP-3, 1000 bp; RP-19, 1250 bp and 1100 bp) and geographical specific bands (RP-9, 650 bp) from RAPD as well as genotype specific band (IS-8, 550 bp) from genotype RGC-1031 (Rajasthan) from ISSR were selected and converted into SCAR markers. The study revealed first set of sequence-based SCAR markers in cluster bean which found more specific information using RAPD and ISSR profiles. One genotype specific SCAR-20 for RGC-1031 (tolerant genotype against Macrophomina phaseolina), could be used to prove identity of the genotype for improvement as well for its genetic purity assessment. Another SCAR-8 was selected due to its specificity for cluster bean genotypes from Rajasthan which might be important for population admixture studies.

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Section: Discussionmentioning

confidence: 99%

A set of SCAR markers in cluster bean (Cyamopsis tetragonoloba L. Taub) genotypes

Sharma¹,

Kumar²,

Raman³

et al. 2014

ABB

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“…These markers are available in abundant numbers, neutral to environmental influence and, therefore, more robust in discriminating among germplasm lines. The molecular markers that have been used in cowpea characterization include restriction fragment length polymorphism (Fatokun et al, 1993), amplified fragment length polymorphism (Coulibaly et al, 2002), random amplified polymorphic DNA (Ba et al, 2004), SSRs (Li et al, 2001), inter SSR analysis (Ajibade et al, 2000), gene derived markers (Wang et al, 2008), and SNPs (Xiong et al, 2016).…”

Section: Exploring the Diversity And Potential Of Cwrs For Introgressmentioning

confidence: 99%

Introgression Breeding in Cowpea [Vigna unguiculata (L.) Walp.]

et al. 2020

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The narrow base of genetic diversity characteristic of cowpea can be attributed to it being self-pollinating, evolving from narrow wild germplasm and exhibiting very limited gene flow between wild and cultivated types. Backcrossing to introduce simply inherited desirable traits and utilization of improved breeding lines and varieties as parents in crossing programs further narrowed the genetic base of cowpea varieties. In most cowpea breeding programs, genes for resistance and market traits were pyramided into lines characterized by high levels of acceptance to farmers and consumers. Besides predisposing widely distributed improved varieties to genetic vulnerability, a narrow base of genetic variation may be contributing to the plateauing in cowpea grain yield, which compromises genetic gains. Cross compatible wild relatives have not been used in variety development because breeders shy away from them due to their tiny seed size, unattractive seed coat color and texture, pod shattering, and susceptibility to viruses. A number of wild cowpea relatives, both within and outside section Catiang of Vigna species, have been evaluated for their reaction to cowpea insect pests and diseases. Vigna vexillata lines were resistant to the legume pod borer (Maruca vitrata), the cowpea weevil (Callosobruchus maculatus), and Striga gesnerioides but are cross incompatible with cultivated cowpea. Some lines among the cross compatible wild relative V. unguiculata ssp. dekindtiana were found to be resistant to aphid in the seedling stage, while others showed good levels of drought and heat tolerance. Molecular markers are being generated to identify quantitative trait loci (QTL) with effects on some desirable attributes in cowpea. Modern breeding tools, including transgenics, can be applied for the improvement of cowpea, bypassing the natural barriers of traditional breeding. Transgenic cowpea with Bt gene cry1Ab showing resistance to M. vitrata has been released in Nigeria. Genome editing, a powerful emerging tool, can also be used for developing improved cowpea varieties with durable resistance to pests and diseases.

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“…Also ISSR technique (Figure 1) discriminate among P cineraria 1 and 4, while RAPD markers (Figure 2) did not. However, Ajibade et al [49] and Galvan et al [15] concluded that ISSR would be a better tool than RAPD for phylogenetic studies. Nagaoka and Ogihara [50] have also reported that the ISSR primers produced several times more information than RAPD markers in wheat.…”

Section: Methodsmentioning

confidence: 99%

DNA Finger Printing of Prosopis cineraria and Prosopis juliflora Using ISSR and RAPD Techniques

Elmeer¹,

Al-Malki²

2011

AJPS

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Cited by 119 publications

References 12 publications

A set of SCAR markers in cluster bean (<i>Cyamopsis tetragonoloba</i> L. Taub) genotypes

A set of SCAR markers in cluster bean (<i>Cyamopsis tetragonoloba</i> L. Taub) genotypes

Introgression Breeding in Cowpea [Vigna unguiculata (L.) Walp.]

DNA Finger Printing of <i>Prosopis cineraria</i> and <i>Prosopis juliflora</i> Using ISSR and RAPD Techniques

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Untitled

Cited by 119 publications

References 12 publications

A set of SCAR markers in cluster bean (&lt;i&gt;Cyamopsis tetragonoloba&lt;/i&gt; L. Taub) genotypes

A set of SCAR markers in cluster bean (&lt;i&gt;Cyamopsis tetragonoloba&lt;/i&gt; L. Taub) genotypes

Introgression Breeding in Cowpea [Vigna unguiculata (L.) Walp.]

DNA Finger Printing of &lt;i&gt;Prosopis cineraria&lt;/i&gt; and &lt;i&gt;Prosopis juliflora&lt;/i&gt; Using ISSR and RAPD Techniques

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Product

Resources

About

A set of SCAR markers in cluster bean (<i>Cyamopsis tetragonoloba</i> L. Taub) genotypes

A set of SCAR markers in cluster bean (<i>Cyamopsis tetragonoloba</i> L. Taub) genotypes

DNA Finger Printing of <i>Prosopis cineraria</i> and <i>Prosopis juliflora</i> Using ISSR and RAPD Techniques