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Broadway, Roxanne M.; Góngora, Carmenza E.; Kain, Wendy; Sanderson, John P.; Monroy, Jose A.; Bennett, K. C.; Warner, Jason; Hoffmann, Michael P.

doi:10.1023/a:1022346301626

Cited by 50 publications

(9 citation statements)

References 23 publications

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“…The effect of feeding larvae on diets amended with MBP/exo or MBP/endo proteins was insect mortality and growth delay, confirming the results obtained by Broadway et al (1998) on H. hampei , who reported 100% insect mortality after 30 days of consumption of a 1% (w/w) mix of chitinolytic enzymes, but improving the efficiency in the case of MBP/exo by reducing the time for 100% mortality to 15 days, with only half the amount of protein, 0.5% (w/w). Meanwhile the MBP/endo exhibited a lower performance, causing 85% mortality after 35 days.…”

Section: Discussionsupporting

confidence: 84%

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In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops

Martínez¹,

Echeverri

Florez³

et al. 2012

AMB Expr

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Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus hampei and the coffee rust fungus Hemileia vastatrix. Both recombinant proteins MBP/exochitinase and MBP/endochitinase showed chitinolytic activity. When recombinant purified proteins were added to an artificial coffee-based diet for the coffee berry borer, MBP/exochitinase at a concentration of 0.5% W/W caused delayed growth of larvae and 100% mortality between days 8 and 15, while MBP/endochitinase caused 100% mortality at day 35. H. vastatrix urediniospores presented total cell wall degradation in their germinative tubes within 18 h of exposure to the proteins at enzyme concentrations of 5 and 6 mg ml-1, with exochitinase having the greatest effect. The dual deleterious effect of S. albidoflavus chitinases on two of the most limiting coffee pests worldwide, the coffee borer and the coffee rust, make them potential elements to be incorporated in integrated control strategies.

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Section: Discussionsupporting

confidence: 84%

“…In addition, the mixture affected the growth, development and/or survival of the budworm Heliothis virescens , the looper Trichoplusia ni , the whitefly Bemisia argentifolii , the potato aphid Myzus persicae , and the coffee berry borer H. hampei (Broadway et al 1998). …”

Section: Introductionmentioning

confidence: 99%

In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops

Martínez¹,

Echeverri

Florez³

et al. 2012

AMB Expr

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“…Chitinases are reported to adversely affect insect pests, together with other natural products like toxins, lectins, proteases, and α-amylase inhibitors (Broadway et al 1998; Kramer and Muthukrishnan 1997; Oyeleye and Normi 2018). In this study, we found distinctive groups of chitinases in maize with different molecular structures and catalytic mechanisms in support of previous studies, and here we focused on exploring the role of endochitinases in maize insect defense.…”

Section: Discussionmentioning

confidence: 99%

Maize Endochitinase Expression in Response to Fall Armyworm Herbivory

Yang

Taylor

Luthe

2021

Preprint

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A large percentage of crop loss is due to insect damage yearly, especially caterpillar damage. Plant chitinases are considered excellent candidates to combat these insects since they can catalyze chitin degradation in peritrophic matrix (PM), an important protective structure in caterpillar midgut. Compared to chemical insecticides, chitinases could improve host plant resistance and be both economically and environmentally advantageous. The focus of this research was to find chitinase candidates that could improve plant resistance by effectively limiting caterpillar damage. Five classes of endochitinase (I-V) genes were characterized in the maize genome, and we further isolated and cloned four chitinase genes (chitinase A, chitinase B, chitinase I, and PRm3) present in two maize (Zea mays L.) inbred lines Mp708 and Tx601, with different levels of resistance to caterpillar pests. Further, we investigated the role of these maize chitinases in response to fall armyworm (Spodoptera frugiperda, FAW) attacks. Results from gene expression and enzyme assays from maize leaves indicated that both chitinase transcript abundance and enzymatic activity increased in response to FAW feeding and mechanical wounding. Furthermore, chitinase retained activity inside the caterpillar’s midgut since specific activity was detected in both the food bolus and frass. When examined under scanning electron microscopy, PMs from Tx601-fed caterpillars showed structural damage when compared to diet controls. Analysis of chitinase transcript abundance after caterpillar feeding and proteomic analysis of maize leaf trichomes in the two inbreds suggested that the chitinase PRm3 in Tx601 has potential insecticidal properties.

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“…Vodovar et al (2006) reported that P. entomophila provides mortality to Drosophila melanogaster due to strong hemolytic activity, which involves proteins, such as lipases, chitinases and/or hydrolases. Furthermore, several researchers have indicated that bacterial chitinases were effective in providing control of insects and mites by hydrolyzing their chitinous exoskeleton (Broadway et al 1998). Wilson et al (2002) reported that bacterial hemolysins are exotoxins that attack blood cell membranes and cause cell rupture.…”

Section: Discussionmentioning

confidence: 99%

Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production

Qessaoui¹,

Bouharroud²,

Amarraque³

et al. 2018

Journal of Plant Protection Research

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The two-spotted spider mite (Tetranychus urticae Koch) is an important pest of many horticultural crops. A study was conducted to evaluate the effect of three fluorescent Pseudomonas isolates obtained from rhizospheric soil of tomato (Solanum lycopersicon) in Agadir, Morocco: Q110B, Q036B and Q172B, as potential biological control agents for T. urticae. Both acaricide and repellent activities were assessed on homogenous adult mites. The acaricidal activity test evaluated five concentrations of bacterial suspensions: 0 (control), 10 2 , 10 4 , 10 6 , 10 8 , and 10 10 cfu ⋅ ml -1 , while only the 10 10 cfu • ml −1 concentration of each bacterium was used for the repellent bioassay. The mortality rate and repellentindex were recorded 24, 48 and 72 h after application. Results indicated that the survival rate of T. urticae was reduced (p ≤ 0.01) by all three bacterial isolates compared to control. Within the 24-72 h time period the mortality rates ranged from 8 to 87%, 16 to 99%, and 13 to 89%, for Q110B, Q036B and Q172B isolates, respectively. The isolate Q036B (LC 50 = 0.598 cfu ⋅ ml -1 ) provided higher mortality rates than Q172B and Q110B with LC 50 values of 90,846 and 169,585 cfu ⋅ ml -1 , respectively. Repellent activity was also the highest with Pseudomonas Q036B having a 71% repellence index at 48 h after application. Regarding the mechanism of action, all three isolates produced hydrogen cyanide, and exhibited protease and cellulose activities, although only Q036B and Q172B had potential chitinase action. Identification analysis showed that the isolates were either Pseudomonas putida (Q172B) or P. fluorescens (Q110B and Q036B). Our results indicate that the P. fluorescens isolate Q036B is a promising candidate for biological control of T. urticae, and has potential to contribute to an integrated pest management program to control this important pest. Then the fruits produced will be qualified as safe for consumers and the environment. The present work was customized to give support for policy decision makers as an agroecological potential meeting needs of industries and ecological balance.

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Cited by 50 publications

References 23 publications

In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops

In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops

Maize Endochitinase Expression in Response to Fall Armyworm Herbivory

Ecological applications of Pseudomonas as a biopesticide to control two-spotted mite Tetranychus urticae: chitinase and HCN production

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