PP-165 Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR

Mojarad, Ehsan Nazemalhosseini; Nejad, Mohammad Rostami; Sahebekhtiari, Navid; Rad, Masoumeh Azimi; Nochi, Zahra; Dabiri, Hossein; Zali, Mohammad Reza; Haghighi, Ali

doi:10.1016/s1201-9712(09)60559-2

Cited by 6 publications

(7 citation statements)

References 14 publications

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“…D’asheesh’s results differed from the present study by reporting the higher prevalence rates of E. dispar than E. histolytica . Similar results were recorded in the central and north-west regions and the Kurdistan province of Iran; Izmir, Turkey; Australia; and north--west Ethiopia ( Dagci et al 2007 ; Fotedar et al 2007b ; Mojarad et al 2010 ; Fallah et al 2014 ; Yimer et al 2017 ; Bahrami et al 2019 ).…”

Section: Discussionsupporting

confidence: 72%

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

Mahmood

Bakr

2020

Polish Journal of Microbiology

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The present study was conducted to evaluate the infection rates of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii among asymptomatic individuals in Erbil City, northern Iraq. The research intent was to discover whether pathogenic or nonpathogenic species cause a high rate of symptomless Entamoeba infections. Stool samples were microscopically examined, and the 18S-rRNA gene was targeted utilizing the nested PCR technique in the positive specimens. Initial results based on morphological features showed that the Entamoeba prevalence rate was 7.4%. Significantly higher rates of infections were seen in females than in males and in low-income people than in moderate-income people. The incidence rates among the asymptomatic individuals, as determined by molecular analysis, were as follows: E. histolytica – 6%, E. dispar – 4.3%, and E. moshkovskii – 0.3%. Of all the Entamoeba positive samples, a single infection with E. histolytica was identified in 41.4% samples; the single infection with E. dispar in 18.6% samples, 35.7% samples had mixed infections with two Entamoeba species, and 4.3% had mixed infections with three species. The current study concluded that 7.4% of healthy people, who live in the endemic area under investigation, carry Entamoeba species asymptomatically. Additionally, the majority of asymptomatic Entamoeba infections were caused by the pathogenic E. histolytica (81.4%) compared to E. dispar (58.6%), and E. moshkovskii with the lowest rate of infection. Single and co-infections with E. histolytica and E. dispar were noted. E. moshkovskii, which was identified for the first time in the region, was only seen in mixed infections.

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Section: Discussionsupporting

confidence: 72%

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

Mahmood

Bakr

2020

Polish Journal of Microbiology

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“…No cross reaction was detected with other luminal protozoa (E. histolytica/dispar, E. coli, B. hominis, G. intestinalis, I. butschlii) as previously reported (25). Our study indicates that E.dispar may be more common in our region than in other countries (26). Recent data point out that E. dispar is perhaps 7-10 times more common than E. histolytica worldwide (27).…”

Section: Discussionsupporting

confidence: 82%

“…These are thought to be other identical amoebas like E. dispar and E. moshkowskii. Some records are available that show the occurrence of these organisms in our country, but no data has been found in this region (4,(22)(23)(24)26). No cross reaction was detected with other luminal protozoa (E. histolytica/dispar, E. coli, B. hominis, G. intestinalis, I. butschlii) as previously reported (25).…”

Section: Discussionmentioning

confidence: 50%

The Investigation of Entamoeba histolytica Prevalence in Some Villages of Sivas by ELISA Method

Malatyalı¹,

Özçelık²,

Çeliksöz³

2011

TurkiyeParazitolDerg

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“…Nazemalhosseini Mojarad et al in a study among patient with gastrointestinal complaint indicated that of 22 microscopy-positive samples for histolytica/E. dispar complex, 21 (95.45%) samples were diagnosed as E. dispar by PCR (33).…”

Section: Discussionmentioning

confidence: 99%

Frequency of Intestinal Parasites in Patients With Gastrointestinal Disorders, in Different Parts of Iran During 2012-2013

Zebardast¹,

Gharavi²,

Abadi³

et al. 2015

Int J Enteric Pathog

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Background:Intestinal parasites of humans are one of the most important health problems worldwide, especially those located in tropical and subtropical areas. Objectives: The aim of this study was to determine the frequency of intestinal parasites in patients with gastrointestinal disorders, in different parts of Iran. Patients and Methods: A total of 1520 stool samples were collected from patients with gastrointestinal disorders. The stool specimens were examined by direct wet mount, formalin-ether concentration and a modified version of the Ziehl-Neelsen staining technique. Amoeba-positive samples were cultured for further differentiation of Entamoeba histolytica, E. dispar, E. moshkovskii. DNA-based methods were used to differentiate these amoebas and to detect Cryptosporidium-positive samples. Statistical analysis was carried out by SPSS ver. 16.Results: Out of the 1520 individuals studied, 153 (10.06%) were infected at least with one intestinal parasite. 781 (51.4%) of patients were male and 738 (48.6%) were female. The prevalence of protozoan parasites 148 (9.7%) was significantly higher than helminth parasites 5 (0.3%) (P < 0.001). The frequency of intestinal parasites was as follows: Blastocystis sp., 72 (4.73%); Giardia intestinalis, 35 (2.30%); Entamoeba coli 21 (1.38%); Endolimax nana 10 (0.92%); Cryptosporidium spp., 1 (0.06%); Entamoeba dispar, 1 (0.06%); Dientamoeba fragilis, 1 (0.06%); Hymenolepis nana, 3 (0.19%); Dicrocoelium dendriticum, 2 (0.13%). In five (0.32%) of the positive samples, co-infections with two parasites were found. G. intestinalis was more prevalent in male 22/35 (62.86%) than female 13/35 (37.14%) as well as in 0-9 years old group. In one sample Heterodera ova contained larva were seen. Conclusions: Blastocystis and G. intestinalis were the predominant intestinal parasites detected in patient with gastrointestinal disorders. The results indicated that the intestinal parasites, particularly helminth infections have been significantly declined in recent years.

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PP-165 Characterization of Entamoeba histolytica and Entamoeba dispar in fresh stool by PCR

Cited by 6 publications

References 14 publications

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

Molecular Identification and Prevalence of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in Erbil City, Northern Iraq

The Investigation of Entamoeba histolytica Prevalence in Some Villages of Sivas by ELISA Method

Frequency of Intestinal Parasites in Patients With Gastrointestinal Disorders, in Different Parts of Iran During 2012-2013

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