PP-10 induces apoptosis via JNK/SPAK activation and STAT3 inhibition in Hepatocarcinoma cells in vitro

Abstract

Background: The detail of mechanism involving in PP-10 anti-cancer activity remains to be elucidated, and the effect on HCC cells is unknown. Methods: MTT and colony formation assays were used to determine the effect of PP-10 on cell growth. Flow cytometry analysis and Hoechst 33258 were used to assess apoptosis. Gene set enrichment analysis (GSEA) was used to explore changes in apoptosis-associated pathways. Western blotting was used to detect protein expression levels. Results: In our study, PP-10 significantly suppressed cancer cell viability while had low toxicity to normal cells, with the HCC cell lines HepG2 and HuH7 being particularly sensitive to PP-10 treatment. PP-10 induced mitochondrial-related apoptosis in HepG2 and HuH7 cells. Moreover, GSEA showed that the MAPK signaling pathway could be correlated with PP-10-induced apoptosis. We used western blotting to confirm that PP-10 induced apoptosis in HepG2 and HuH7 cells by modulating the JNK/SPAK signaling and inhibiting the STAT3 signaling pathway. Conclusion: Collectively, our results show that PP-10 induces apoptosis via the JNK/SPAK and STAT3 signaling pathways in HepG2 and HuH7 hepatocarcinoma cells.

Effect of white lion goose serum on gastric cancer cells

“…Propidium iodide (PI) is a fluorescent dye that can bind to DNA. It dyes necrotic cells and cells that lose cell membrane integrity in late apoptosis (An et al, 2022), as visualized by red fluorescence. For necrotic cells, Annexin V can enter the cytoplasm because of the lack of cell membrane integrity and combine with PS located inside the cell membrane, as visualized by green fluorescence.…”

Effects of Cirsium setidens extract on gene expression related to apoptosis induction and metastasis inhibition in human breast cancer

“…Cirsium setidens is a perennial herb of the Asteraceae family and has been used as a medicine and food because it is effective for hypertension, antitumor, antioxidant, and hepatoprotective (Shin et al, 2022). C. setidens contains bioactive compounds such as silymarin and is known to have an apoptotic effect in neuroblastoma in addition to liver protection, but studies on the induction of apoptosis in breast cancer cell are relatively insufficient (An et al, 2022;Lee et al, 2014). Therefore, in this study, in order to confirm the inhibitory effect on proliferation and metastasis of breast cancer cell from CSE, gene expression inducing breast cancer cell apoptosis was analyzed to confirm the anticancer mechanism, and major compound in C. setidens was analyzed to provide basic data for uses such as anticancer-related health functional foods or pharmaceutical compound in the future.…”