pox-neuro is required for development of chemosensory bristles inDrosophila

Awasaki, Takeshi; Kimura, Ken‐ichi

doi:10.1002/(sici)1097-4695(19970620)32:7<707::aid-neu6>3.0.co;2-8

Cited by 81 publications

(89 citation statements)

References 37 publications

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“…Accordingly, we used the Pox neuro (Poxn) null mutant Poxn ΔM22-B5 , whose taste bristles are either missing or are transformed into bristles that lack gustatory innervations but retain the mechanosensory innervation (24). However, Poxn is a gene with pleiotropic activities, which also include functions in the central nervous system and the development of antenna, legs, and male genitalia (24)(25)(26)(27)(28). Hence, for our studies, we compared Poxn ΔM22-B5 mutants that carry the complete rescue construct with Poxn ΔM22-B5 mutants that carry rescue constructs that lack enhancer elements required for the formation of either a subset of (labellar) or most taste bristles (Fig.…”

Section: Significancementioning

confidence: 99%

Positive and negative gustatory inputs affect Drosophila lifespan partly in parallel to dFOXO signaling

Ostojić

Boll

Waterson³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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In Caenorhabditis elegans, a subset of gustatory neurons, as well as olfactory neurons, shortens lifespan, whereas a different subset of gustatory neurons lengthens it. Recently, the lifespan-shortening effect of olfactory neurons has been reported to be conserved in Drosophila. Here we show that the Drosophila gustatory system also affects lifespan in a bidirectional manner. We find that taste inputs shorten lifespan through inhibition of the insulin pathway effector dFOXO, whereas other taste inputs lengthen lifespan in parallel to this pathway. We also note that the gustatory influence on lifespan does not necessarily depend on food intake levels. Finally, we identify the nature of some of the taste inputs that could shorten versus lengthen lifespan. Together our data suggest that different gustatory cues can modulate the activities of distinct signaling pathways, including different insulin-like peptides, to promote physiological changes that ultimately affect lifespan.sensory system | insulin signaling | physiology | gustatory receptors | aging A ging is a universal process that causes deterioration in the biological functions of an organism over the progression of its lifetime. This process is affected by genetic and environmental factors, whose interaction could be mediated by the sensory system, which perceives and transmits environmental information to modulate the signaling activities of downstream target tissues. Accordingly, external sensory cues and sensory neuron activities have been shown to alter the lifespan of both Caenorhabditis elegans and Drosophila melanogaster (1-6).In C. elegans, the laser ablation of a specific subset of gustatory or olfactory neurons extends lifespan, whereas ablation of a different subset of gustatory neurons shortens it (1). Interestingly, at least part of this sensory influence on lifespan has also been observed in other animals. In Drosophila, impairment of olfaction through a mutation in Or83b, which encodes a broadly expressed atypical odorant receptor (7), increases lifespan (3). In addition, exposure of dietary-restricted flies to food odors, like live yeast, can partly suppress their long-life phenotype (3). The conservation of the olfactory influence on lifespan is thus consistent with the possibility that gustatory inputs will also bidirectionally alter the lifespan of both C. elegans and D. melanogaster.The effects of sensory neurons on C. elegans lifespan have been shown to be partly mediated by insulin/IGF signaling (1, 2, 8). The insulin/IGF pathway also affects fly lifespan: down-regulation of the activities of the insulin receptor InR and the receptor substrate, CHICO, extends lifespan (9, 10). Moreover, an increase in activity of the downstream transcription factor dFOXO, which is negatively regulated by both InR and CHICO, increases fly lifespan (11,12). Consistent with these observations, mutations in several of the Drosophila insulin-like peptide (dilp) genes (13), which are expressed in the median neurosecretory cells (mNSCs) in the fly brain (14-1...

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Section: Significancementioning

confidence: 99%

Positive and negative gustatory inputs affect Drosophila lifespan partly in parallel to dFOXO signaling

Ostojić

Boll

Waterson³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Gustatory bristles are present on the primary taste structures: the labellum, front legs, wing margins, and the ovipositor (20). To test if gustatory neurons mediate egg-laying preference, we assayed pox-neuro (poxn) mutants, in which taste bristles are transformed into mechanosensory bristles lacking gustatory receptors (21,22); null mutants also have defects in the central nervous system (23). Homozygous poxn ⌬M22-B5 females showed reduced egg-laying preference (OI ϭ ϩ0.28; Fig.…”

Section: Egg-laying Preference For and Positional Aversion To Aa-contmentioning

confidence: 99%

Oviposition preference for and positional avoidance of acetic acid provide a model for competing behavioral drives in Drosophila

Joseph¹,

Devineni²,

King

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

194

235

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Selection of appropriate oviposition sites is essential for progeny survival and fitness in generalist insect species, such as Drosphila melanogaster, yet little is known about the mechanisms regulating how environmental conditions and innate adult preferences are evaluated and balanced to yield the final substrate choice for eggdeposition. Female D. melanogaster are attracted to food containing acetic acid (AA) as an oviposition substrate. However, our observations reveal that this egg-laying preference is a complex process, as it directly opposes an otherwise strong, default behavior of positional avoidance for the same food. We show that 2 distinct sensory modalities detect AA. Attraction to AA-containing food for the purpose of egg-laying relies on the gustatory system, while positional repulsion depends primarily on the olfactory system. Similarly, distinct central brain regions are involved in AA attraction and repulsion. Given this unique situation, in which a single environmental stimulus yields 2 opposing behavioral outputs, we propose that the interaction of egg-laying attraction and positional aversion for AA provides a powerful model for studying how organisms balance competing behavioral drives and integrate signals involved in choice-like processes.choice behavior ͉ gustatory system ͉ olfactory system ͉ mushroom body ͉ ellipsoid body O viposition provides a powerful yet simple means for monitoring preference behavior in Drosophila melanogaster, since a laid egg represents a marker for female position. Past studies have used egg laying as a readout for conditions advantageous to progeny development (1, 2), in which oviposition preference effectively separates larvae of different sibling species of Drosophila. Egg laying has also been used to detect aversion toward compounds toxic to both larvae and adults (3, 4). Furthermore, numerous studies have used patterns of oviposition to distinguish subtle differences in host plant preferences, which have provided insights into resource requirements and ecological behaviors of different Drosophila species (5, 6).Despite numerous studies using oviposition-site selection as a behavioral readout, direct study of the relevant sensory circuits and the oviposition program itself have been initiated only recently in D. melanogaster (7,8). To investigate the genetic mechanisms and neural circuits regulating this important behavioral choice in D. melanogaster, we developed a simple yet robust 2-choice assay that utilizes acetic acid (AA), a naturally occurring product of fruit fermentation, as an egg-laying attractant (9, 10). However, in addition to verifying a strong egg-laying preference for AA, we surprisingly observed D. melanogaster show a strong positional aversion to the same AA-containing food. We demonstrate that when sampling for oviposition sites, females integrate input from distinct sensory modalities to choose a particular behavioral output from 2 competing options: ovipositional attraction for and positional repulsion to AA. Egg-laying preference is p...

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“…In previous studies, poxn null mutants were found to be completely devoid of sweet sensory input [38,39], whereas the GRN blocking flies may have partially retained the ability to sense sweetness because not all of the sugar sensing neurons were blocked by using one Gr-Gal4 line [24,30]. This led to the enhanced water consumption (Figure 2A).…”

Section: Discussionmentioning

confidence: 87%

“…The following flies were used: poxn 70 [38], poxn ΔM22 [39], UAS-dORK [40], Gr5a-Gal4, Gr61a-Gal4, Gr64a-Gal4, Gr64c-Gal4, Gr64d-Gal4, Gr64e-Gal4, Gr64f-Gal4 [41], UAS-dTrpA1 [42], UAS-mCD8::GFP [43], NP115-Gal4, NP883-Gal4, NP1076-Gal4 (Drosophila Genetic Resource Center).…”

Section: Experimental Animalsmentioning

confidence: 99%

“…which taste bristles are transformed into mechanosensory bristles lacking gustatory receptors [38,47,48]. As a result of the absence of gustatory attraction to sucrose, poxn mutant flies displayed no preference in the two-choice CAFE assay ( Figure 2B), as evident by their dramatically lowered consumption of sucrose solution (P<0.001).…”

Section: Blocking the Taste Sensory System Eliminates Sucrose Preferencementioning

confidence: 99%

See 1 more Smart Citation

Identification of neurons responsible for feeding behavior in the Drosophila brain

Sun

Wang

Zhou

et al. 2014

Sci. China Life Sci.

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Drosophila melanogaster feeds mainly on rotten fruits, which contain many kinds of sugar. Thus, the sense of sweet taste has evolved to serve as a dominant regulator and driver of feeding behavior. Although several sugar receptors have been described, it remains poorly understood how the sensory input is transformed into an appetitive behavior. Here, we used a neural silencing approach to screen brain circuits, and identified neurons labeled by three Gal4 lines that modulate Drosophila feeding behavior. These three Gal4 lines labeled neurons mainly in the suboesophageal ganglia (SOG), which is considered to be the fly's primary taste center. When we blocked the activity of these neurons, flies decreased their sugar consumption significantly. In contrast, activation of these neurons resulted in enhanced feeding behavior and increased food consumption not only towards sugar, but to an array of food sources. Moreover, upon neuronal activation, the flies demonstrated feeding behavior even in the absence of food, which suggests that neuronal activation can replace food as a stimulus for feeding behavior. These findings indicate that these Gal4-labeled neurons, which function downstream of sensory neurons and regulate feeding behavior towards different food sources is necessary in Drosophila feeding control.feeding behavior, sugar-sensing neurons, SOG, CAFE assay, proboscis extension response (PER) Citation:Sun F, Wang YJ, Zhou YQ, van Swinderen B, Gong ZF, Liu L. Identification of neurons responsible for feeding behavior in the Drosophila brain.

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pox-neuro is required for development of chemosensory bristles inDrosophila

Cited by 81 publications

References 37 publications

Positive and negative gustatory inputs affect Drosophila lifespan partly in parallel to dFOXO signaling

Positive and negative gustatory inputs affect Drosophila lifespan partly in parallel to dFOXO signaling

Oviposition preference for and positional avoidance of acetic acid provide a model for competing behavioral drives in Drosophila

Identification of neurons responsible for feeding behavior in the Drosophila brain

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