Powers of differentiation of clonal strains of bone marrow fibroblasts

Gerasimov, Yu. V.; Fridenshtein, A. Ya.; Chailakhyan, R. K.; Shishkova, V. V.

doi:10.1007/bf00839611

Cited by 10 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings may be compared with the differentiation potential previously described for BMSC clones. When mouse and rabbit clones were transplanted in vivo within diffusion chambers, approximately one‐third formed bone and cartilage, whereas the remaining clones only formed fibrous tissue [29, 30]. By in vitro assay, a number of human clones were restricted to osteogenic differentiation, where most clones exhibited bipotential (osteo‐adipo or osteo‐chondro), with a smaller proportion demonstrating a capacity for multipotential (osteo‐chondro‐adipo) [31, 32].…”

Section: Discussionmentioning

confidence: 99%

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

et al. 2007

View full text Add to dashboard Cite

Using a variety of cell separation techniques and cultivation conditions, circulating, adherent, connective tissue, clonogenic cells were found in just 3 donors out of 66, demonstrating that these precursors are extremely rare in postnatal human blood. Contrary to humans, guinea pig blood shows much more reproducible connective tissue colony formation; it was therefore chosen to study the differentiation potential of adherent blood-derived clonogenic cells. Out of 22 single colony-derived strains of various morphologies, only 5 spindle-shaped strains showed extensive proliferative capacity in vitro. None of these strains formed bone upon in vivo transplantation, whereas two strains formed cartilage in high-density pellet cultures in vitro. Both chondrogenic strains included cells expressing aggrecan, whereas nonchondrogenic strains did not. Out of four polyclonal strains studied, one formed both cartilage and abundant bone accompanied by hematopoiesis-supporting stroma. Evidently, there are cells in adult guinea pig blood capable of both extensive proliferation and differentiation toward cartilage: circulating chondrogenic precursors. Although some of these cells lack osteogenic potential and therefore represent committed chondrogenic precursors, others may be multipotential and consequently belong to the family of skeletal stem cells. This is the first demonstration of postnatal circulating chondrogenic precursors, as well as of precursor cells with chondrogenic but not osteogenic potential.

show abstract

Section: Discussionmentioning

confidence: 99%

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

et al. 2007

View full text Add to dashboard Cite

show abstract

“…About 20% of the colonies formed bone when individual colonies of mouse or guinea pig BMSCs were transplanted . When rabbit single-colonyderived BMSC strains were transplanted into diffusion chambers, either autogenously or into immunocompromised mice, bone was formed by 48.3% (Gerasimov et al, 1986;Friedenstein et al, 1987) and 36.8% of the strains (Bennett et al, 1991), respectively.…”

Section: (C) Transplantation Of Clonal Bmsc Strainsmentioning

confidence: 99%

Bone Marrow Stromal Cells: Characterization and Clinical Application

Krebsbach

Kuznetsov

Bianco

et al. 1999

Critical Reviews in Oral Biology & Medicine

241

198

View full text Add to dashboard Cite

and 3Dipartimento di Medicina Sperimentale, Universita dell Aquila, L'Aquila, Italy; #corresponding author *Dedicated to the memory of Professor Alexander J. Friedenstein, pioneer of bone marrow stromal cell research, inspiring teacher and friend.ABSTRACT: The bone marrow stroma consists of a heterogeneous population of cells that provide the structural and physiological support for hematopoietic cells. Additionally, the bone marrow stroma contains cells with a stem-cell-like character that allows them to differentiate into bone, cartilage, adipocytes, and hematopoietic supporting tissues. Several experimental approaches have been used to characterize the development and functional nature of these cells in vivo and their differentiating potential In vitro. In vivo, presumptive osteogenic precursors have been identified by morphologic and immunohistochemical methods. In culture, the stromal cells can be separated from hematopoietic cells by their differential adhesion to tissue culture plastic and their prolonged proliferative potential. In cultures generated from single-cell suspensions of marrow, bone marrow stromal cells grow in colonies, each derived from a single precursor cell termed the colony-forming unit-fibroblast. Culture methods have been developed to expand marrow stromal cells derived from human, mouse, and other species. Under appropriate conditions, these cells are capable of forming new bone after in vivo transplantation. Various methods of cultivation and transplantation conditions have been studied and found to have substantial influence on the transplantation outcome. The finding that bone marrow stromal cells can be manipulated in vitro and subsequently form bone in vivo provides a powerful new model system for studying the basic biology of bone and for generating models for therapeutic strategies aimed at regenerating skeletal elements,

show abstract

“…Furthermore, it has been demonstrated that CFU-F populations are not homogeneous but rather contain a hierarchy of progenitors including multipotential SSCs and committed progenitors. By in vivo analyses of clonal strains established by single CFU-Fs, SSCs constitute from one-tenth to one-third of all CFU-Fs (Bennett et al, 1991; Chailakhian et al, 1978; Gerasimov Iu et al, 1986; Kuznetsov et al, 1997b, 2004). To date, there are no markers that can be used prospectively to separate multipotential CFU-Fs, or SSCs, from more committed CFU-Fs.…”

Section: Introductionmentioning

confidence: 99%

Enumeration of the colony-forming units–fibroblast from mouse and human bone marrow in normal and pathological conditions

et al. 2009

View full text Add to dashboard Cite

Bone marrow stromal cell populations, containing a subset of multipotential skeletal stem cells, are increasingly contemplated for use in tissue engineering and stem cell therapy, whereas their involvement in the pathogenetic mechanisms of skeletal disorders is far less recognized. We compared the concentrations of stromal clonogenic cells, colony forming units–fibroblast (CFU-Fs), in norm and pathology. Initially, culture conditions were optimized by demonstrating that fetal bovine serum heat inactivation could significantly repress colony formation. Using non-heat-inactivated fetal bovine serum, the concentration of CFU-Fs (colony-forming efficiency, CFE) ranged from 3.5 ± 1.0 to 11.5 ± 4.0 per 1 × 105 nucleated cells in five inbred mouse strains. In four transgenic lines with profound bone involvement, CFE was either significantly reduced or increased compared to wild-type littermates. In normal human donors, CFE decreased slightly with age and averaged 52.2 ± 4.1 for children and 32.3 ± 3.0 for adults. CFE was significantly altered in patients with several skeletal, metabolic, and hematological disorders: reduced in congenital generalized lipodystrophy, achondroplasia (SADDAN), pseudoachondroplasia, and Paget disease of bone and elevated in alcaptonuria and sickle cell anemia. Our findings indicate that under appropriate culture conditions, CFE values may provide useful insights into bone/bone marrow pathophysiology.

show abstract

Powers of differentiation of clonal strains of bone marrow fibroblasts

Cited by 10 publications

References 2 publications

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs

Bone Marrow Stromal Cells: Characterization and Clinical Application

Enumeration of the colony-forming units–fibroblast from mouse and human bone marrow in normal and pathological conditions

Contact Info

Product

Resources

About