Powdery Mildew Resistance in Tomato by Impairment of SlPMR4 and SlDMR1

Huibers, Robin P.; Loonen, Annelies E. H. M.; Gao, Dongling; Ackerveken, Guido Van den; Visser, Richard G. F.; Bai, Yuling

doi:10.1371/journal.pone.0067467

Cited by 86 publications

(119 citation statements)

References 26 publications

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“…We are currently verifying whether silencing tomato orthologs of these Arabidopsis S-genes leads to resistance to O. neolycopersici. Our results till now showed that (1) Arabidopsis dmr1 and pmr4 mutants are resistant to O. neolycopersici and, (2) silencing SlDmr1 and SlPmr4 in tomato results also in resistance to O. neolycopersici (Huibers et al 2013). Together with the tomato ol-2 mutant (Slmlo1), our results demonstrate that orthologs of Arabidopsis S-genes are present in crops and disruption in their S-gene function leads to resistance to different pathogens.…”

Section: Nonhost-like Resistance Conferred By Editing Plant S-genessupporting

confidence: 53%

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

et al. 2013

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Powdery mildews (PMs) cause disease in a wide range of plant species including important crops. Taking tomato as an example, here we review findings on the genetic basis and mechanisms of plant resistance to PMs. First, we present a summary of our research on tomato resistance to two PM species, with the focus on Oidium neolycopersici. We discuss the genetics of resistance to this pathogen in tomato. Then, we compare different forms of resistance mediated by different resistance genes based on molecular and cytological data. Also, we provide a comparison between these resistance genes in tomato with those in barley, Arabidopsis and wheat, in order to present a model for the genetic basis of resistance to PMs in plants. We try to accommodate these resistance mechanisms in the current model of plant innate immunity. At the end we discuss possibilities to translate these findings to practical approaches in breeding for resistance to PMs in crops.

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Section: Nonhost-like Resistance Conferred By Editing Plant S-genessupporting

confidence: 53%

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

et al. 2013

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“…Two S-genes for downy mildew, DMR1 and DMR6, have been described in Arabidopsis and tomato [12,[19][20][21]. dmr1 and dmr6 mutants induce resistance to DM by accumulating elevated levels of homoserine [21] and 2OG-Fe(II) oxygenase [20], respectively.…”

Section: Journal Of Phylogenetics and Evolutionary Biologymentioning

confidence: 99%

“…Therefore, a loss of function in MLO genes consequently induce resistance to PM [10,15]. PMR genes on the other hand are far less studied and most are putative, but some have been shown to play a role in PM susceptibility in Arabidopsis (AtPMR4, AtPMR5, and AtPMR6) and tomato (SlPMR4) [16][17][18][19]. PMR genes are involved in cell wall biology where they mediate structure formation and pectin accumulation [18].…”

Section: Introductionmentioning

confidence: 99%

Candidate Susceptibility Genes for Powdery and Downy Mildew in Watermelon and Squash

Porterfield¹,

Meru²

2017

J Phylogenetics Evol Biol

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Powdery mildew (PM) caused by Podosphaera xanthii and downy mildew (DM) caused by Pseudoperonospora cubensis are two of the most economically important diseases for watermelon (Citrullus lanatus) and squash (Cucurbita pepo, C. maxima and C. moschata). Traditional breeding for resistance to PM and DM is resource intensive, often requiring decades' long phenotyping and selection processes. As an alternative, durable and broadspectrum resistance to PM and DM can be obtained through loss-of-function of susceptibility genes in elite breeding material. Susceptibility genes for PM [Mildew-Locus-O (MLO) and Powdery Mildew Resistance (PMR)] and DM [Downy Mildew Resistance (DMR)] have been functionally proven in model plant species. Previous studies have reported candidate MLO genes for C. lanatus and C. pepo, but none for C. maxima and C. moschata. On the contrary, no PMR or DMR candidate genes have been identified for C. lanatus or any of the Cucurbita species. The current study used bioinformatics approaches based on sequence similarity, phylogenetic relationships and presence of conserved domains to predict candidate MLO genes in C. maxima and C. moschata and PMR and DMR genes in C. lanatus, C. pepo, C. maxima and C. moschata. Four MLO homologs in C. maxima and five in C. moschata clustered within Clade V, a clade containing all MLO susceptibility genes in dicots, and had highly conserved transmembrane domains and C-terminal PM interaction motif. Sixty-three candidate PMR genes were identified among the four species, 16 of which had close similarity to functionally proven PMR homologs in model species. Similarly, 37 candidate DMR genes were identified 12 among which clustered with functionally proven DMR homologs in model species. Functional analysis of the genes identified in the current study will reveal their role in pathogenesis and assess their potential for manipulation through gene editing methods to generate novel resistant plant genotypes.

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“…We collected peroxidase gene (AY373372; García-Gutiérrez et al 2013) and Arabidopsis Powdery Mildew Resistant 4 (PMR4) gene encoding callose synthase (protein AEE82336.1; Huibers et al 2013) from the literature. Then we blasted these two gene sequences in the Melon Genome Database 'Melonomics' (https://melonomics.net/) and retrieved two peroxidases (Prx) and one glucan synthase (GLS)-like genes.…”

Section: In Silico Analysis Of Peroxidase and Glucan Synthase-like Genesmentioning

confidence: 99%

Characterization and Expression Analysis of Peroxidases and Glucan Synthase Like Genes in Cucumis melo L

Howlader

Sumi

Kim

et al. 2016

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Department of Agricultural Education, Sunchon National University, Suncheon 57922, Korea ABSTRACT Powdery mildew (PM) is a severe fungal disease for melon cultivation worldwide. Stress resistance related genes could be important tools to address this problem. In this study, we retrieved defense related peroxidase and glucan synthase genes from Melon Genome Database 'Melonomics'. Thereafter, we analyzed the genes in silico. We conducted protein blast in the NCBI database and found a high degree of homology among them. Based on the highest protein homology we named two isoforms of Cucumis melo peroxidase 2-like genes (CmPrx2-1 and CmPrx2-2) and one glucan synthase1-like gene (CmGLS1). In reverse transcriptionpolymerase chain reaction (PCR), all 3 genes showed organ specific expression in a C. melo line, SCNU1154. Real-time quantitative PCR expression of these 3 genes was conducted in the infected leaf samples by PM fungus Podosphaera xanthii and also treated leaf samples by exogenous phytohormones (salicylic acid and methyl jasmonate). The CmPrx2-2 gene was up-regulated in response to all seven races of PM fungus whereas up-regulation or down-regulation of CmPrx2-1 gene was race-specific. The CmGLS1 gene was down-regulated in response to all races except one race. The CmPrx2-1, CmPrx2-2, and CmGLS1 genes were up-regulated under both salicylic acid and methyl jasmonate treatments but their level of expression was higher in salicylic acid treated plants compared to methyl jasmonate. Therefore, we speculate that defense response of the three tested genes is largely mediated by the salicylic acid signaling pathway under PM infection. Taken together, the data presented herein may be useful resources in the development of PM stress resistant in C. melo L.

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Powdery Mildew Resistance in Tomato by Impairment of SlPMR4 and SlDMR1

Cited by 86 publications

References 26 publications

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

Genetics and molecular mechanisms of resistance to powdery mildews in tomato (Solanum lycopersicum) and its wild relatives

Candidate Susceptibility Genes for Powdery and Downy Mildew in Watermelon and Squash

Characterization and Expression Analysis of Peroxidases and Glucan Synthase Like Genes in Cucumis melo L

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