Powdery mildew pathogens can suppress the chitinase gene expression induced in detached inner epidermis of barley coleoptile

Fujita, K.; Matsuda, Yoshinori; Wada, Masaaki; Hirai, Yoshito; Mori, Keisuke; Moriura, Nobuyuki; Nonomura, Teruo; Kakutani, Koji; Toyoda, Hideyoshi

doi:10.1007/s00299-004-0866-z

Cited by 9 publications

(2 citation statements)

References 17 publications

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“…We found that a subtilisin‐chymotrypsin inhibitor (CI‐1B, M0XBS5) was significantly upregulated in C2 (3.57‐fold), whereas another subtilisin‐chymotrypsin inhibitor (CI‐2A, A0A287EI14) was dramatically downregulated in C2 (20‐fold). Chitinases are the hydrolytic enzymes that protect plants against pathogens (Fujita et al., 2004; Toyoda et al., 1991). Two chitinases (D2CVR3 and F2CSS7) were expressed at lower levels in C2.…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

et al. 2021

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Hullessbarley (Hordeum vulgare L.) is a barley cultivar, serving as one of the major food crops worldwide. The main nutrients in hulless barley are starch, protein, and βglucan, the contents of which vary considerably among different cultivars. However, the potential molecular mechanisms that underlie these variations in nutrient are still insufficiently clear. In this study, the nutrient content of two hulless barley cultivars ('Zangqing 2000' and 'C2') was analyzed, and quantitative tandem mass tag-LC-MS/MS proteomics and bioinformatics analysis were used to verify the differentially expressed proteins (DEPs) between the two cultivars. Parallel reaction monitoring (PRM) analysis was used to verify the expression change of candidate DEPs. The content of starch and amylose in C2 was lower than that in Zangqing 2000, and βglucan, protein content, and amylopectin showed a higher concentration in C2 hulless barley cultivar. A total of 326 DEPs were identified, with 108 induced proteins and 218 reduced proteins in C2 compared with Zangqing 2000. Further functional and pathways analysis of DEPs revealed that C2 hulless barley cultivar has relatively more biological activities, and DEPs in C2 hulless barley cultivar were mainly associated with the biological processes of multiple important metabolic pathways. Moreover, PRM analysis showed the accuracy level of quantitative proteomic data. Overall, this was the first study that undertook the proteomic analysis in Zangqing 2000 and C2 and the results revealed the molecular mechanisms of seed nutrient biosynthesis and accumulation, which provide key insights about the development of hulless barley quality.

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Section: Discussionmentioning

confidence: 99%

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

et al. 2021

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“…To efficiently analyse the interactions, methods are needed to isolate the intracellular content of a single plant cell attacked by a fungal appressorium and/or haustorium. At present, only two techniques are available: micropipette manipulation [39] and laser microdissection [40]. However, these techniques are not widely used because they require considerable skill and specialised equipment.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

et al. 2022

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Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths of the plant epidermis, are suitable for monitoring infection processes of powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On the trichomes, tomato powdery mildew (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced a maximum of four vigorously elongated hyphae per conidium, which stopped growth approximately 12 days after inoculation. Single trichome cells, invaded by fungal hyphae at various fungal infection stages during the 12-day period after the inoculation of single conidia, were cut at the bases and directly collected with small precision scissors (i.e., microscissors) held by the manipulator under a DM. Subsequently, suc-polymerase chain reaction (PCR) (reverse transcription (RT)-PCR followed by nested (N)-PCR) was conducted to explore gene expression in the infected trichome. We selected intron-containing genes from tomatoes and powdery mildew fungi for the detection of constitutive gene transcripts, namely plasma membrane H+-ATPase (LHA2) and β-tubulin 2 (TUB2) genes. In suc-PCR, a single band from spliced mRNAs of both LHA2 and TUB2 genes were detected, suggesting that both genes were successfully transcribed in single KTP-03-infected trichomes. With combined primers for both LHA2 and TUB2 (multiplex RT-PCR/N-PCR), two bands were detected through the amplification of intron-spliced mRNAs of both genes. Therefore, our single-trichome cell PCR amplification method is effective for detecting the expression patterns of genes from both tomato and powdery mildew fungus. Combinations of digital microscopy, microscissors, and multiplex RT-PCR/N-PCR amplification techniques will be useful for simultaneously analysing the molecular interactions between plants and powdery mildew fungi at the level of single tomato leaf trichome cells. Also, this employed technique will be of benefit in other plant species and crops, possessing leaf trichome cells, to elucidate the molecular interactions between plants and pathogens.

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Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens

et al. 2017

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Powdery mildew pathogens can suppress the chitinase gene expression induced in detached inner epidermis of barley coleoptile

Cited by 9 publications

References 17 publications

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

Simultaneous Detection of Plant- and Fungus-Derived Genes Constitutively Expressed in Single Pseudoidium neolycopersici-Inoculated Type I Trichome Cells of Tomato Leaves via Multiplex RT-PCR and Nested PCR

Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens

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