Potide-G Derived from Potato (Solanum tuberosum L.) Is Active against Potato Virus YO (PVYO) Infection

Tripathi, Giri Raj; Park, Jaekyun; Park, Young-Min; Hwang, Indeok; Park, Yoonkyung; Hahm, Kyung‐Soo; Cheong, Hyeonsook

doi:10.1021/jf061794p

Cited by 17 publications

(12 citation statements)

References 43 publications

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“…Results of real-time PCR showed that the application of 10 μg of purified potide-G was sufficient to reduce virus accumulation by 50% on average from the PVY infection in susceptible “Winter Valley” cultivars. The authors have reported that this peptide isolated from resistant potatoes offers new opportunities for the development of new biological pesticides against plant viruses (Tripathi et al, 2006). …”

Section: Introductionmentioning

confidence: 99%

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

et al. 2013

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Cationic antimicrobial peptides (AMPs) and host defense peptides (HDPs) show vast potential as peptide-based drugs. Great effort has been made in order to exploit their mechanisms of action, aiming to identify their targets as well as to enhance their activity and bioavailability. In this review, we will focus on both naturally occurring and designed antiviral and antitumor cationic peptides, including those here called promiscuous, in which multiple targets are associated with a single peptide structure. Emphasis will be given to their biochemical features, selectivity against extra targets, and molecular mechanisms. Peptides which possess antitumor activity against different cancer cell lines will be discussed, as well as peptides which inhibit virus replication, focusing on their applications for human health, animal health and agriculture, and their potential as new therapeutic drugs. Moreover, the current scenario for production and the use of nanotechnology as delivery tool for both classes of cationic peptides, as well as the perspectives on improving them is considered.

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Section: Introductionmentioning

confidence: 99%

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

et al. 2013

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“…The entire inoculation was conducted using a syringe to reduce damage to the leaf surface. The 'Winter Valley' variant was shown to be highly susceptible to PVY O infection with the development of local disease symptoms, expressed as the appearance of leaf burching and crinkling, mild to strong yellow leaf mosaic, necrotic veins, leaf spots, and leaf dropping . However, the infected 'Winter Valley'-G cultivar did not show any symptoms after PVY O infection, over the entire life of the plant (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…When S. tuberosum L. 'Golden Valley' was infected with PVY O , the Ry gene was highly up-regulated and the CP gene could not be detected via real-time PCR and ELISA . Moreover, we also obtained sufficient results for antiviral activity from the application of the 'Golden Valley' protein and potide-G, a small (5.57 kDa) antiviral peptide isolated from the 'Golden Valley' cultivar . Therefore, we have focused on the 'Golden Valley' Ry gene ( G-Ry ) in an effort to improve resistance against PVY O infection for agricultural applications.…”

Section: Discussionmentioning

confidence: 99%

“…We determined that the 'Golden Valley' variant was the most resistant to PVY O infection among the 32 Korean potato cultivars; conversely, the 'Winter Valley' variant was identified as the most susceptible. We also observed sufficient results for antiviral activity from the application of the 'Golden Valley' protein and potide-G, a small (5.57 kDa) antiviral peptide isolated from the 'Golden Valley' cultivar . Here, we report the genetic transformation of the potato (cv.…”

Section: Introductionmentioning

confidence: 98%

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Expression of G-Ry Derived from the Potato (Solanum tuberosum L.) Increases PVY^o Resistance

Lee

Park

Hwang

et al. 2010

J. Agric. Food Chem.

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In Solanaceae, potato virus Y(O) (PVY(O)) is a widespread virus leading to severe damages such as necrosis, molting, and yield reduction. The resistance Y gene (Ry gene) of potato specifically confers resistance to PVY infection. Previously, potatoes resistant to PVY(O) infection were screened among the 32 Korean cultivars. 'Golden Valley' displayed the most resistance to PVY(O) infection. 'Golden Valley''s Ry gene (G-Ry) was cloned from 'Golden Valley', and the function was investigated. G-Ry protein contains 1134 amino acid residues and is structurally similar to the Y-1, which confers resistance to PVY infection in Solanum tuberosum subsp. andigena. To generate a PVY(O)-resistant potato, the G-Ry gene has been introduced into 'Winter Valley', the cultivar most susceptible to PVY(O) infection among the 32 Korean cultivars. Transgenic 'Winter Valley' ('Winter Valley'-G) showed an increased resistance to PVY infection. This approach may ultimately lead to the development of a virus-resistant plant.

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“…Inhibition of germination of hyphae and fungal spore was the possible mechanism of AHPs's antifungal activity (Fernańdez et al, 2012). In 2006, two antiviral peptides named potide-G and golden peptide were ) that showed promising antiviral activity against potato virus YO (PVYO) (Tripathi et al, 2006). Another study with C. annum found a new antimicrobial protein CaAMP1 that exhibited promising activity against both different bacteria (MICs 5-30 µg/ml) and fungi (MICs 5-100 µg/ml).…”

Section: Plant Name Traditional Uses Referencesmentioning

confidence: 99%

Ethnobotany and Antimicrobial Peptides From Plants of the Solanaceae Family: An Update and Future Prospects

et al. 2020

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The Solanaceae is an important plant family that has been playing an essential role in traditional medicine and human nutrition. Members of the Solanaceae are rich in bioactive metabolites and have been used by different tribes around the world for ages. Antimicrobial peptides (AMPs) from plants have drawn great interest in recent years and raised new hope for developing new antimicrobial agents for meeting the challenges of antibiotic resistance. This review aims to summarize the reported AMPs from plants of the Solanaceae with possible molecular mechanisms of action as well as to correlate their traditional uses with reported antimicrobial actions of the peptides. A systematic literature study was conducted using different databases until August 2019 based on the inclusion and exclusion criteria. According to literature, a variety of AMPs including defensins, protease inhibitor, lectins, thionin-like peptides, vicilin-like peptides, and snaking were isolated from plants of the Solanaceae and were involved in their defense mechanism. These peptides exhibited significant antibacterial, antifungal and antiviral activity against organisms for both plant and human host. Brugmansia, Capsicum, Datura, Nicotiana, Salpichora, Solanum, Petunia, and Withania are the most commonly studied genera for AMPs. Among these genera, Capsicum and the Solanum ranked top according to the total number of studies (35%-38% studies) for different AMPs. The mechanisms of action of the reported AMPs from Solanaceae was not any new rather similar to other reported AMPs including alteration of membrane potential and permeability, membrane pore formation, and cell aggregation. Whereas, induction of cell membrane permiabilization, inhibition of germination and alteration of hyphal growth were reported as mechanisms of antifungal activity. Plants of the Solanaceae have been used traditionally as antimicrobial, insecticidal, and antiinfectious agents, and as poisons. The reported AMPs from the Solanaceae are the products of chemical shields to protect plants from microorganisms

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Potide-G Derived from Potato (Solanum tuberosum L.) Is Active against Potato Virus Y^O (PVY^O) Infection

Cited by 17 publications

References 43 publications

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

Expression of G-Ry Derived from the Potato (Solanum tuberosum L.) Increases PVY^o Resistance

Ethnobotany and Antimicrobial Peptides From Plants of the Solanaceae Family: An Update and Future Prospects

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Potide-G Derived from Potato (Solanum tuberosum L.) Is Active against Potato Virus YO (PVYO) Infection

Cited by 17 publications

References 43 publications

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

Current scenario of peptide-based drugs: the key roles of cationic antitumor and antiviral peptides

Expression of G-Ry Derived from the Potato (Solanum tuberosum L.) Increases PVYo Resistance

Ethnobotany and Antimicrobial Peptides From Plants of the Solanaceae Family: An Update and Future Prospects

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Potide-G Derived from Potato (Solanum tuberosum L.) Is Active against Potato Virus Y^O (PVY^O) Infection

Expression of G-Ry Derived from the Potato (Solanum tuberosum L.) Increases PVY^o Resistance