Potentiometric urea biosensor utilizing nanobiocomposite of chitosan-iron oxide magnetic nanoparticles

Ali, Akbar; AlSalhi, Mohamad S.; Ansari, Anees A.; Israr, Muhammad; Sadaf, J. R.; Ahmed, Ezzat A.; Nur, Omer; Willander, Magnus

doi:10.1088/1742-6596/414/1/012024

Cited by 45 publications

(25 citation statements)

References 20 publications

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“…Iron oxide nanoparticle (Fe 3 O 4 ) being chemically and biologically neutral have been coated with catalysts, enzymes, or even antibodies to be used as biosensors [151]. In a recently published paper, Chauhan et al [152] have modified Fe 3 O 4 nanoparticle using poly(indole-5-carboxylicacid) by preparing nanobiocomposite for its use as a sensor for the determination of pesticides such as malathion and chlorpyrifos in a wide range of concentrations (0.1–70 nm).…”

Section: Reviewmentioning

confidence: 99%

Biogenic Fabrication of Iron/Iron Oxide Nanoparticles and Their Application

et al. 2016

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Enshrined in this review are the biogenic fabrication and applications of coated and uncoated iron and iron oxide nanoparticles. Depending on their magnetic properties, they have been used in the treatment of cancer, drug delivery system, MRI, and catalysis and removal of pesticides from potable water. The polymer-coated iron and iron oxide nanoparticles are made biocompatible, and their slow release makes them more effective and lasting. Their cytotoxicity against microbes under aerobic/anaerobic conditions has also been discussed. The magnetic moment of superparamagnetic iron oxide nanoparticles changes with their interaction with biomolecules as a consequence of which their size decreases. Their biological efficacy has been found to be dependent on the shape, size, and concentration of these nanoparticles.

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Section: Reviewmentioning

confidence: 99%

Biogenic Fabrication of Iron/Iron Oxide Nanoparticles and Their Application

et al. 2016

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“…[14] Among various metal oxide nanoparticles, iron oxide nanoparticles (Fe 3 O 4 NP) have been found to be chemically and biologically inert; they can be coated with metal catalysts, enzymes or antibodies to increase their functionalities for biosensor applications. [15] Moreover, Fe 3 O 4 NP have been proposed to provide a favourable micro-environment for immobilisation of desired enzymes directly onto electrode surface resulting in catalytic activity, electron transfer between medium and electrode, or improved direct electron transfer of redox proteins.…”

Section: Introductionmentioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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A new electrochemical sensing device was constructed for determination of pesticides. In this report, acetylcholinesterase was bioconjugated onto hybrid nanocomposite, i.e. iron oxide nanoparticles and poly(indole-5-carboxylic acid) (Fe 3 O 4 NPs/Pin5COOH) was deposited electrochemically on glassy carbon electrode. Fe 3 O 4 NPs was showed as an amplified sensing interface at lower voltage which makes the sensor more sensitive and specific. The enzyme inhibition by pesticides was detected within concentrations ranges between 0.1À60 and 1.5À70 nM for malathion and chlorpyrifos, respectively, under optimal experimental conditions (sodium phosphate buffer, pH 7.0 and 25 C). Biosensor determined the pesticides level in water samples (spiked) with satisfactory accuracy (96%À100%). Sensor showed good storage stability and retained 50% of its initial activity within 70 days at 4 C.

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“…Increasing in urea level in blood and urine can cause urinary tract obstruction, dehydration, shock, burns, and gastrointestinal bleeding, whereas reducing in urea level may be seen in nephritic syndrome, cachexia and hepatic failure [2]. The assay of urea can be followed by many different techniques as spectrometry [3][4][5], potentiometry [6][7][8][9], conductometry [10][11][12], coulometry [13] and amperometry [14]. Nowadays several attempts have been widely done to develop urea electrochemical biosensor, due to their inherent advantages such as robustness, easy miniaturization, excellent detection limits, and requirement for very small amounts of analyte [15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%