Potentiometric titrations of Bacillus subtilis cells to low pH and a comparison of modeling approaches

Fein, Jeremy B.; Boily, Jean-François; Yee, Nathan; Gorman‐Lewis, Drew; Turner, Benjamin F.

doi:10.1016/j.gca.2004.07.033

Cited by 196 publications

(193 citation statements)

References 24 publications

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“…The experimental titration data show that T. zilligii cell suspensions possess substantial buffering capacity over the range from pH 3 to 10 ( capacity of cell suspensions observed to be reversible, reproducible and similarly related to pH and biomass concentration (Fein et al, 1997;Daughney and Fein, 1998;Daughney et al, 1998Daughney et al, , 2001Cox et al, 1999;Yee and Fein, 2001;Fein et al, 2005;Burnett et al, 2006a;Hetzer et al, 2006;Heinrich et al, 2007Heinrich et al, , 2008). …”

Section: Proton Adsorption By T Zilligiimentioning

confidence: 94%

“…Firstly, SCMs were used to derive model curves for proton and metal adsorption by previously studied mesophilic and thermophilic species and consortia. This was achieved by using Westall et al, 1995;Fein et al, 2005) were included in the modelling where available. Comparison of SCM curves that did or did not consider the initial proton condition was achieved by evaluating the change in adsorption over the pH range of interest, as opposed to the absolute amount of adsorption.…”

Section: Surface Complexation Modellingmentioning

confidence: 99%

“…This involves using the previously published SCMs to predict proton and Cd adsorption for standard conditions as described above (1 g comparison of the adsorptive properties of thermophiles and mesophiles, because several studies have shown that various types and configurations of electrostatic and non-electrostatic SCMs can provide a good fit to experimental data for proton and metal adsorption by bacteria (Daughney and Fein, 1998;Fein et al, 2005). For example, some of the previously published SCMs invoke four different types of surface sites, but may cover a broader range of pH than we employ for the comparisons made in the present investigation.…”

Section: Surface Complexation Modellingmentioning

confidence: 99%

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Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents

et al. 2010

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Adsorption by microorganisms can play a significant role in the fate and transport of metals in natural systems. Surface complexation models (SCMs) have been applied extensively to describe metal adsorption by mesophilic bacteria, and several recent studies have extended this framework to thermophilic bacteria. We conduct acid-base titrations and batch experiments to characterise proton and Cd adsorption onto the thermophilic archaeon Thermococcus zilligii. The experimental data and the derived SCMs indicate that the archaeon displays significantly lower overall sorption site density compared to previously studied thermophilic bacteria such Anoxybacillus flavithermus, Geobacillus stearothermophilus, G. thermocatenulatus, and Thermus thermophilus. The thermophilic bacteria and archaea display lower sorption site densities than the mesophilic microorganisms that have been studied to date, which points to a general pattern of total concentration of cell wall adsorption sites per unit biomass being inversely correlated to growth temperature.

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Section: Proton Adsorption By T Zilligiimentioning

confidence: 94%

Section: Surface Complexation Modellingmentioning

confidence: 99%

Section: Surface Complexation Modellingmentioning

confidence: 99%

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Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents

et al. 2010

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“…Titration experiments were performed (in duplicate) according to the procedures described by Fein et al (2005). The suspensions (1 g L −1 , 40 mL) of montmorillonite and B. subtilis and the composite in 0.01 M NaNO 3 were placed in a sealed titration vessel maintained under a positive pressure of N 2 .…”

Section: Potentiometric Titrationsmentioning

confidence: 99%

Surface complexation modeling of Cd(II) sorption to montmorillonite, bacteria, and their composite

Wang

Huang

et al. 2016

Biogeosciences

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Abstract. Surface complexation modeling (SCM) has emerged as a powerful tool for simulating heavy metal adsorption processes on the surface of soil solid components under different geochemical conditions. The component additivity (CA) approach is one of the strategies that have been widely used in multicomponent systems. In this study, potentiometric titration, isothermal adsorption, zeta potential measurement, and extended X-ray absorption fine-structure (EXAFS) spectra analysis were conducted to investigate Cd adsorption on 2 : 1 clay mineral montmorillonite, on Grampositive bacteria Bacillus subtilis, and their mineral-organic composite. We developed constant capacitance models of Cd adsorption on montmorillonite, bacterial cells, and mineralorganic composite. The adsorption behavior of Cd on the surface of the composite was well explained by CA-SCM. Some deviations were observed from the model simulations at pH < 5, where the values predicted by the model were lower than the experimental results. The Cd complexes of X 2 Cd, SOCd + , R-COOCd + , and R-POCd + were the predominant species on the composite surface over the pH range of 3 to 8. The distribution ratio of the adsorbed Cd between montmorillonite and bacterial fractions in the composite as predicted by CA-SCM closely coincided with the estimated value of EXAFS at pH 6. The model could be useful for the prediction of heavy metal distribution at the interface of multicomponents and their risk evaluation in soils and associated environments.

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“…The suspensions were equilibrated for 12 h and then, a small amount of base (0.2 ml of 1 M NaOH) was added to deprotonate a significant part of the charged sites, rendering the surface negative, without exceeding pH 10 for the suspension of activated sludge. This was chosen because above pH 10, significant cell lysis occurs, and may interfere with the buffering measurements (Fein et al, 2005).…”

Section: Potentiometric Mass Titrationmentioning

confidence: 99%

The use of Potentiometric Mass Titration (PMT) technique for determining the acid-base behavior of activated sludge

2015

Global NEST Journal

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Activated sludge is a biological floc composed of microorganisms, organics, and inorganics and plays an important role in wastewater treatment. The pH of an activated sludge suspension determines the acid -base behavior and the charge of the microorganisms in water. In the present study, the determination of acid -base behavior of activated sludge was performed using microelectrophoresis and Potentiometric Mass Titrations (PMT). For activated sludge that is a complicated biological system, direct microelectrophoresis is not a suitable method for the determination of its ζ-potential. This method leads to a variety of isoelectric point (iep) values due to the presence of different microorganisms in activated sludge. On the contrary, the PMT method used for the first time for activated sludge can provide the overall point of zero charge (pzc) value that is 8.2. In addition, a simple methodology is proposed for the quantitative determination of the overall solid charge as a function of solution pH, which requires only (a) the titration curves of an electrolyte suspension and of the blank electrolyte solution and (b) the calculation of the H + ions consumed for the above titration curves.

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Potentiometric titrations of Bacillus subtilis cells to low pH and a comparison of modeling approaches

Cited by 196 publications

References 24 publications

Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents

Proton and cadmium adsorption by the archaeon Thermococcus zilligii: Generalising the contrast between thermophiles and mesophiles as sorbents

Surface complexation modeling of Cd(II) sorption to montmorillonite, bacteria, and their composite

The use of Potentiometric Mass Titration (PMT) technique for determining the acid-base behavior of activated sludge

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