Potentiometric, kinetic, and thermodynamic investigations into Cu<sup>2+</sup> ion binding properties of vinyl imidazole containing IMAC adsorbent

Developing ultrasensitive chemical sensors with small scale and fast response through simple design and low‐cost fabrication is highly desired but still challenging. Herein, a simple and universal sensing platform based on a hydrogel interferometer with femtomol‐level sensitivity in detecting (bio)chemical molecules is demonstrated. A unique local concentrating effect (up to 109 folds) in the hydrogel induced by the strong analyte binding and large amount of ligands, combined with the signal amplification effect by optical interference, endows this platform with an ultrahigh sensitivity, specifically 10−14m for copper ions and 1.0 × 10−11 mg mL−1 for glycoprotein with 2–4 order‐of‐magnitude enhancement. The specific chemical reactions between selected ligands and target analytes provide high selectivity in detecting complex fluids. This universal principle with broad chemistry, simple physics, and modular design allows for high performance in detecting wide customer choices of analytes, including metal ions and proteins. The scale of the sensor can be down to micrometer size. The nature of the soft gel makes this platform transparent, flexible, stretchable, and compatible with a variety of substrates, showing high sensing stability and robustness after 200 cycles of bending or stretching. The outstanding sensing performance grants this platform great promise in broad practical applications.

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“…The binding between the ligands and Cu 2+ takes only a few minutes. [18] The diffusivity of ions and water molecules in a hydrogel…”

Section: Hydrogelsmentioning

confidence: 99%

Hydrogel Interferometry for Ultrasensitive and Highly Selective Chemical Detection

et al. 2018

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Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Chen

et al. 2016

J of Separation Science

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The simultaneous determination of two binding parameters for metal ions on an immobilized metal affinity chromatography column was performed by frontal chromatography. In this study, the binding parameters of Cu(2+) to l-glutamic acid were measured, the metal ion-binding characteristics of the complex ligand were evaluated. The linear correlation coefficients were all greater than 99%, and the relative standard deviations of two binding parameters were 0.58 and 0.059%, respectively. The experiments proved that the frontal chromatography method was accurate, reproducible, and could be used to determine the metal-binding parameters of the affinity column. The effects of buffer pH, type, and concentration on binding parameters were explored by uniform design experiment. Regression, matching and residual analyses of the models were performed. Meanwhile, the optimum-binding conditions of Cu(2+) on the l-glutamic acid-silica column were obtained. Under these binding conditions, observations and regression values of two parameters were similar, and the observation values were the best. The results demonstrated that high intensity metal affinity column could be effectively prepared by measuring and evaluating binding parameters using frontal chromatography combined with a uniform design experiment. The present work provided a new mode for evaluating and preparing immobilized metal affinity column with good metal-binding behaviors.

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Highly effective adsorption of copper ions by poly(vinyl imidazole) cryogels

et al. 2020

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Potentiometric, kinetic, and thermodynamic investigations into Cu²⁺ ion binding properties of vinyl imidazole containing IMAC adsorbent

Cited by 4 publications

References 34 publications

Hydrogel Interferometry for Ultrasensitive and Highly Selective Chemical Detection

Hydrogel Interferometry for Ultrasensitive and Highly Selective Chemical Detection

Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Highly effective adsorption of copper ions by poly(vinyl imidazole) cryogels

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Potentiometric, kinetic, and thermodynamic investigations into Cu2+ ion binding properties of vinyl imidazole containing IMAC adsorbent

Cited by 4 publications

References 34 publications

Hydrogel Interferometry for Ultrasensitive and Highly Selective Chemical Detection

Hydrogel Interferometry for Ultrasensitive and Highly Selective Chemical Detection

Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Highly effective adsorption of copper ions by poly(vinyl imidazole) cryogels

Contact Info

Product

Resources

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Potentiometric, kinetic, and thermodynamic investigations into Cu²⁺ ion binding properties of vinyl imidazole containing IMAC adsorbent