Potentiometric determination of salicylhydroxamic acid (urinary struvite stone inhibitor) based on the inhibition of urease activity

New, simple, sensitive and validated spectrophotometric method for the determination of salicylhydroxamic acid in capsules and raw material according to the ICH guidelines

“…Standard solutions showed good linearity (r 2 > 0.9999) over the concentration range tested. The sensitivity of the current method is higher compared with the reported spectrophotometric one (Salem, 2003;[11] the LOQ was 1.53 lg mL À1 ) or even the potentiometric one reported by Hassan et al, 1997 (the LOD was 0.1 lg mL À1 ) [15]. The LOD for SHAM was 0.031 lg mL À1 , while the LOQ was 0.098 lg mL À1 .…”

“…SHAM was additionally evaluated by measuring the absorbance of its V(V) complex at 620 nm and limit of detection was 50 lM [14]. Moreover, potentiometric method for the estimation of SHAM in light of the inhibition of urease action was likewise conducted [15]. A linear calibration curve between 0.5 and 7 lg mL À1 with a limit of detection of 0.1 lg mL À1 was achieved.…”

Struvite as a Non-Equilibrium Solid Phase in Electrolytic Systems (Aqueous Media)

“…Struvite stones are formed in presence of urease-producing bacteria (most commonly Proteus and Klebsiella), resulting from the infection [19][20][21][22][23][24]. Ureases are metalloenzymes catalyzing the hydrolysis of urea into carbon dioxide and ammonia [25][26][27][28][29] (NH 2 ) 2 CO + H 2 O = CO 2 + 2NH 3 (18) This reaction occurs effectively at pH ca. 7, where [NH 4…”

Novel potentiometric approach in glucose biosensor using silver nanoparticles as redox marker

“…The experiments were repeated three times for each point. The initial slope method described by Hassan et al [19][20][21][22] was used in all measurements. The EMF was continuously monitored every 3 s (defined as an interval time).…”