Potentiometric biosensor for acrylamide determination in wastewater using wild type amidase fromPseudomonas aeruginosa

Abstract

Acrylamide is a toxic amide with potentially hazardous effects on the environment and human health. This paper reports the results regarding the development of a potentiometric biosensor in order to determine the amount of this amide in wastewater samples. The biosystem consisted of whole cells of Pseudomonas aeruginosa containing intracellular amidase activity which hydrolyses acrylamide producing ammonium ion and acrylic acid. The cells were immobilized on the surface of several types of membranes such as polyethersulfone, nitrocellulose and nylon, in the presence of glutaraldehyde as bifunctional reagent, and then attached to the surface of an ammonium ion selective electrode. Polyethersulfone was revealed to be the most adequate in terms of biosensor response. The effect of glutaraldehyde concentration was also studied and 5% (v/v) was chosen as the optimum concentration value. The results obtained revealed excellent analytical characteristics of the biosensor such as good linear response in the range of 0.5 to 100mM of acrylamide, a detection limit of 4.48 x 10 -5 M, a response time of 55 s, a sensitivity of 58.9mV/mM. This system was also tested in real samples of complex matrix, namely wastewater from an industrial plant where an average substrate recover of 93.3% was obtained.

Smart Sanitation—Biosensors as a Public Health Tool in Sanitation Infrastructure

“…Lab experiments conducted in ideal conditions, especially those that tested artificially prepared samples, would require further testing of biosensors for direct use in wastewater and sewage systems. While papers often cited that maintenance needs of biosensors were less intensive than current methods, smart toilets and other sensors often require regular cleaning [ 31 , 41 ]. One paper specifically states that, “Most of the bacterial biosensors that are currently available are large, require electronics or are too complicated to operate in [developing] countries” [ 25 ].…”

Acrylamide in food products and the role of electrochemical biosensors in its detection: a comprehensive review

“…Tests with real industrial effluents demonstrated efficient substrate recovery, reinforcing the utility of the biosensor in monitoring wastewater quality and industrial pollution control, with a focus on applying electrochemical methods for precise and efficient detection. 78 However, despite the successful immobilization strategy of Pseudomonas cells leading to a biosensor with a good response time and remarkable sensitivity, there is a concern about potential premature cell loss, a longer device half-life, and better storage resistance of the membrane.…”