Potentiation of oxygen toxicity by menadione in Saccharomyces cerevisiae

Chaput, Mariella; Brygier, Jeanne; Lion, Yves; Sels, André

doi:10.1016/s0300-9084(83)80132-1

Cited by 24 publications

(24 citation statements)

References 27 publications

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“…Consistent with a role in oxidative stress protection, the yeast CUP1 gene is transcriptionally activated when cells are grown in the presence of high oxygen tensions or during respiration (Tamai et al 1993). Here we demonstrate that menadione (vitamin K3) , a pro-oxidant that generates O2"-through redox cycling (Thor et al 1982;Chaput et al 1983), potently activates CUP1 transcription and this transcription is mediated by the heat shock factor (HSF). We show that a single functional copy of the CUP1 gene protects cells from menadione toxicity in an HSF-dependent manner.…”

mentioning

confidence: 78%

“…As shown by primer extension analysis in Figure 1, treatment of S. cerevisiae cells with increasing concentrations of menadione results in a large induction of C UP1 mRNA occurring at a threshold level of menadione (400 ~M), with maximum induction (-50-fold) at 500 ~M menadione. The magnitude of the response to menadione decreases at higher concentrations, with decreased cell viability (data not shown), presumably attributable to free radical-mediated macromolecular damage at these concentrations of menadione (Chaput et al 1983;Gralla and Valentine 1991;Halliwell 1994).…”

Section: Superoxide Radical Generation Induces Cup 1 Transcriptionmentioning

confidence: 95%

“…The yeast CUP1 gene is transcriptionally activated when cells are grown in high oxygen concentrations or during enforced respiration, two conditions known to generate oxidative stress (Tamai et al 1993). To ascertain whether yeast cells activate CUP1 gene transcription in response to a direct oxidative stress, we exposed cells to menadione, a derivative of vitamin K 3 that generates superoxide anion through redox cycling (Hassan and Fridovich 1979;Chaput et al 1983). As shown by primer extension analysis in Figure 1, treatment of S. cerevisiae cells with increasing concentrations of menadione results in a large induction of C UP1 mRNA occurring at a threshold level of menadione (400 ~M), with maximum induction (-50-fold) at 500 ~M menadione.…”

Section: Superoxide Radical Generation Induces Cup 1 Transcriptionmentioning

confidence: 99%

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Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription.

Liu¹,

Thiele²

1996

Genes Dev.

151

153

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Nietallothioneins (NiTs} are a class of low-molecular-weight, cysteine-rich metal-binding proteins that function in metal detoxification and oxidative stress protection. We demonstrate that transcription of the Saccharomyces cerevisiae NIT gene CUP1 is strongly activated by the superoxide anion generator menadione. This activation is exacerbated in a strain lacking the gene encoding Cu, Zn superoxide dismutase (SOD1). CUP1 transcriptional activation by oxidative stress is dependent on a functional CUP1 promoter heat shock element (HSE) and the carboxy-terminal trans-activation domain of heat shock transcription factor (HSF). Furthermore, protection against oxidative stress conferred by CUP1 in a sodlA strain requires HSF-mediated CUP1 transcription. Although in response to heat, HSF-mediated CUP1 transcription and HSF phosphorylation are transient, both CUP1 gene expression and HSF phosphorylation are sustained in response to oxidative stress. Moreover, the patterns of tryptic phosphopeptides resolved from HSF derived from cells subjected to heat shock or oxidative stress are distinct. These results demonstrate that transcription of the S. cerevisiae metallothionein gene under conditions of oxidative stress is mediated by HSF and that in response to distinct activation stimuli, HSF is differentially phosphorylated in a manner that parallels metallothionein gene transcription.

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mentioning

confidence: 78%

Section: Superoxide Radical Generation Induces Cup 1 Transcriptionmentioning

confidence: 95%

Section: Superoxide Radical Generation Induces Cup 1 Transcriptionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription.

Liu¹,

Thiele²

1996

Genes Dev.

151

153

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“…CELL triggered when cells sense an increase in ROS (9). Several redox-cycling agents (menadione and plumbagin) are known to sharply increase intracellular levels of superoxide anions (7,30). In S. cerevisiae, pretreatment with menadione induces an adaptive response that protects yeast cells from a subsequent challenge with hydrogen peroxide; however, cells treated with hydrogen peroxide are unable to survive menadione exposure (29).…”

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confidence: 99%

Candida albicans -Conditioned Medium Protects Yeast Cells from Oxidative Stress: a Possible Link between Quorum Sensing and Oxidative Stress Resistance

Westwater¹,

2005

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Candida albicans, the most frequent fungal pathogen of humans, encounters high levels of oxidants following ingestion by professional phagocytes and through contact with hydrogen peroxide-producing bacteria. In this study, we provide evidence that C. albicans is able to coordinately regulate the oxidative stress response at the global cell population level by releasing protective molecules into the surrounding medium. We demonstrate that conditioned medium, which is defined as a filter-sterilized supernatant from a C. albicans stationary-phase culture, is able to protect yeast cells from both hydrogen peroxide and superoxide anion-generating agents. Exponential-phase yeast cells preexposed to conditioned medium were able to survive levels of oxidative stress that would normally kill actively growing yeast cells. Heat treatment, digestion with proteinase K, pH adjustment, or the addition of the oxidant scavenger alpha-tocopherol did not alter the ability of conditioned medium to induce a protective response. Farnesol, a heat-stable quorum-sensing molecule (QSM) that is insensitive to proteolytic enzymes and is unaffected by pH extremes, is partly responsible for this protective response. In contrast, the QSM tyrosol did not alter the sensitivity of C. albicans cells to oxidants. Relative reverse transcription-PCR analysis indicates that Candida-conditioned growth medium induces the expression of CAT1, SOD1, SOD2, and SOD4, suggesting that protection may be mediated through the transcriptional regulation of antioxidant-encoding genes. Together, these data suggest a link between the quorum-sensing molecule farnesol and the oxidative stress response in C. albicans.

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“…Thus JA300 (pSox-GFP) is appropriate to assess low concentrations (in a range from 1 ppb to 1 ppm) of methyl violgen with high detection sensitivity. JA300 (pSox-GFP) also produced Sox-GFP in the presence of menadione which is also a superoxide aniongenerating reagent 1,10) (data not shown). However, the transformant showed low fluorescence in the medium containing menadione at concentrations higher than 15 M. The growth of JA300 (pSox-GFP) was inhibited remarkably with menadione at 500 M. This result indicates that the Sox-GFP system is highly specific to detect methyl viologen.…”

Section: Notementioning

confidence: 90%

Convenient and Sensitive Evaluation of a Superoxide Anion-Generating Reagent Methyl Viologen byEscherichia coliHarboring asoxS’::gfpReporter Plasmid

Shibuya¹,

Tsukagoshi²,

Ohtsu³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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We constructed a reporter system to detect a superoxide-generating methyl viologen using SoxRS of Escherichia coli and GFP of Aequorea victoria. E. coli carrying this plasmid exhibited strong fluorescence when grown in the presence of a superoxide-generating reagent methyl viologen. The fluorescence intensity observed in the stationary phase culture of the transformant increased in response to the methyl viologen concentration in a range of 0.01 M to 10 M.

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Potentiation of oxygen toxicity by menadione in Saccharomyces cerevisiae

Cited by 24 publications

References 27 publications

Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription.

Oxidative stress induced heat shock factor phosphorylation and HSF-dependent activation of yeast metallothionein gene transcription.

Candida albicans -Conditioned Medium Protects Yeast Cells from Oxidative Stress: a Possible Link between Quorum Sensing and Oxidative Stress Resistance

Convenient and Sensitive Evaluation of a Superoxide Anion-Generating Reagent Methyl Viologen byEscherichia coliHarboring asoxS’::gfpReporter Plasmid

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