Potentiation of K252a, a Protein-Kinase Inhibitor-Induced Polyploidization by cAMP in Cultured Fibrosarcoma Cell Line

Zong, Zhi-ping; Fujikawa-Yamamoto, Kohzaburo; Teraoka, Kohei; Yamagishi, H; Tanino, Mikio; Odashima, Shizuo

doi:10.1006/bbrc.1994.2728

Cited by 23 publications

(18 citation statements)

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“…Polyploidization of mammalian cells occurs in various organs, particularly in the aged or partially hepatectomized liver; however, the mechanisms of polyploidization are poorly understood (Mossin et al 1994, Zong et al 1994, Fogt and Nanji 1996, Seglen 1997. We established tetraploid and octaploid Meth-A cell lines from diploid and tetraploid Meth-A cells, respectively, that had been highly polyploidized by demecolcine (Fujikawa-Yamamoto et al 2001, 2003a.…”

Section: Discussionmentioning

confidence: 99%

Production of Polyploid Meth-A Cells Whose Ploidy is not a Power of 2 by Staurosporine

2004

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SummaryTo examine ploidy change in hyperploid cells after treatments of staurosporine (SS), an inhibitor of protein kinases, octaploid Meth-A cells were exposed to SS, and then the drug concentration was gradually reduced. Seventy cell clones were obtained from the cell population, and they were classified into 4 groups based on their DNA content corresponding to hexa-, octa-, dodeca-and hexadecaploid cells. Durations of G 1 , S and G 2 /M phases of the cells were almost the same as that of parent octaploid cells. The cell volumes of 6-and 12-ploid cells were not proportional to DNA content. It was concluded that polyploid Meth-A cells whose ploidy is not a power of 2 were produced from octaploid cells by SS treatments.

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Section: Discussionmentioning

confidence: 99%

Production of Polyploid Meth-A Cells Whose Ploidy is not a Power of 2 by Staurosporine

2004

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“…K-252a, a potent inhibitor of protein kinases C and I (Zollner, 1993), and demecolcine polyploidize many cultured cells, though their role in polyploidization is still unknown (Zong et al, 1994, Zong et al, 1995. V79 cells were Exponentially growing V79 cells were exposed to K-252a and demecolcine for 4 days at concentrations of 800 and 270 nM, respectively.…”

Section: Discussionmentioning

confidence: 99%

Different Responses of Polyploidized V79 Cells after Removal of Two Drugs, Demecolcine and K-252a.

Fujikawa-Yamamoto¹,

Yamagishi²,

Zong³

et al. 2000

Cell Struct. Funct.

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ABSTRACT. To examine whether or not cells polyploidized by different mechanisms behave in a different manner after drug removal, V79 Chinese hamster cells were assessed by flow cytometry (FCM) after their polyploidization by demecolcine and K-252a, inhibitors of spindle-fiber formation and protein kinase, respectively. Cell cycle analysis of DNA histograms of V79 cells before and after the drug release was performed. With both drugs, the ploidy of V79 cells increased just after the drug removal and was maintained for a week. A difference was evident 10 days after the release. Tetraploid cells were the main population from 10 to 18 days after the release of K-252a, but not demecolcine. Cell cycle parameters were almost the same in pseudo diploid and tetraploid V79 cells, except for the tetraploid S phase which was 2h longer.Key words: cell cycle analysis/V79 cells/polyploidization/K-252a/demecolcine V79 Chinese hamster lung cells were polyploidized at the same rate by demecolcine and K-252a, inhibitors of protein kinases and spindle-fiber formation in M phase, respectively (Fujikawa-Yamamoto et al., 1993), but their morphology differed between multi-and mono-nuclei detected in the cells polyploidized by demecolcine and K-252a, respectively (Fujikawa-Yamamoto et al., 1999b). It is of interest to determine whether or not the cells polyploidized by different mechanisms behave in a different manner after the drug removal.A relationship between the rate of DNA synthesis and DNA content has been reported for Chinese hamster cells by Graves and McMillan (1984) wherein the duration of S phase is almost constant regardless of the DNA content. This was supported by the finding of increased BrdU uptake in polyploid CHO cells (Takanari et al., 1985). Although several studies have reported a constant duration of the S phase in the polyploidization of cultured cells (Brenneisen et al., 1994;Fujikawa-Yamamoto et al., 1997b;Graves et al., 1984;Jordan et al., 1996;Usui et al., 1991;Watters et al., 1994;Zhang et al., 1996), except for Meth-A cells (Fujikawa-Yamamoto et al., 1997b), the cell cycle parameters, that is, the duration of G1, S and G2/M phases, of polyploid cells in a steady state of growth have not been studied well because of difficulty in achieving such a state.In this study, the behavior of V79 cells polyploidized by demecolcine and K-252a was examined by flowcytometry (FCM) for about one month after the removal of the drugs and the cell cycle parameters determined for the pseudo diploid and tetraploid. Materials and Methods CellsV79 (Chinese hamster lung cell line) cells were maintained in a humidified atmosphere of 5% CO2 at 37°C as a monolayer culture in Leibovitz's L15 : Ham's F10 mixture (7:3) supplemented with 10% fetal calf serum (M.A. Bioproducts, Walkersville, Md, USA), streptomycin (100 µg/ml) and penicillin (50 units/ml). The cells were cultured at low density.

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“…This drug polyploidized Meth-A cells, as well as several cell lines (3)(4)(5)(6)15). Although the mechanism of polyploidization is little understood (14,19,20), it requires a high enough drug concentration to arrest cells in Mphase. Demecolcine at a critical concentration that cannot completely block cellcycle progression at Mphase forces the cells to undergo apoptosis (2,12).…”

Section: Discussionmentioning

confidence: 99%

Spontaneous Polyploidization Results in Apoptosis in a Meth-A Tumor Cell Line.

Fujikawa-Yamamoto¹,

Zong²,

Murakami³

et al. 1997

Cell Struct. Funct.

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Potentiation of K252a, a Protein-Kinase Inhibitor-Induced Polyploidization by cAMP in Cultured Fibrosarcoma Cell Line

Cited by 23 publications

References 0 publications

Production of Polyploid Meth-A Cells Whose Ploidy is not a Power of 2 by Staurosporine

Production of Polyploid Meth-A Cells Whose Ploidy is not a Power of 2 by Staurosporine

Different Responses of Polyploidized V79 Cells after Removal of Two Drugs, Demecolcine and K-252a.

Spontaneous Polyploidization Results in Apoptosis in a Meth-A Tumor Cell Line.

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