Potentiation of epinephrine-induced lipolysis in fat cells from estrogen-treated rats

Benoît, Vincent; Valette, Annick; Mercier, Laurence; Meignen, J.M.; Boyer, J.

doi:10.1016/0006-291x(82)91902-7

Cited by 39 publications

(24 citation statements)

References 13 publications

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“…6. This effect of estradiol on the BW is considered to be ascribed, at least in part, to the suppression of body fat accumulation [9][10][11][12][13][14]. The results of the present study clearly show that these inhibitory effects of estradiol on the FI and the BW gain are exerted throughout the day without any appreciable difference in the extent of suppression between the light and dark phases.…”

Section: Discussionsupporting

confidence: 58%

Estradiol-Induced Inhibition of Body Weight Gain and Reduction of Food Intake Are Not Associated with Diurnal Rhythmicity.

Miura

Kikusui

Takeuchi

et al. 1999

Journal of Reproduction and Development

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Abstract. Ovariectomized female rats were subcutaneously implanted with silicone capsules containing estradiol (n=11), which maintained physiological levels of plasma estradiol, or cholesterol (n=10) as control at the age of 9 weeks. Body weight, amount of food intake and water consumption were recorded twice daily during a 2 week period starting one week before the capsule implantation. Estradiol treatment induced suppression of daily food intake and body weight gain in animals carrying estradiol capsules as compared with those carrying cholesterol one, whereas there was no apparent estradiol effect on water intake. The extent of suppression of both body weight gain and food intake were indistinguishable between the light and the dark phases. The present results suggest that the mechanism which mediates the inhibition of body weight gain and the reduction of food intake is not directly linked with that generating diurnal rhythmicity.

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Section: Discussionsupporting

confidence: 58%

Estradiol-Induced Inhibition of Body Weight Gain and Reduction of Food Intake Are Not Associated with Diurnal Rhythmicity.

Miura

Kikusui

Takeuchi

et al. 1999

Journal of Reproduction and Development

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“…A sample (500 mg) of the tissue was homogenized with a Teflon-glass homogenizer in 1 ml of solution A (50 mM NH 4 Cl and 20 mg=ml heparin, pH 8.2) at 10 C. The homogenate was sonicated briefly at 10 C and then used to prepare an acetone=ether powder as described previously. 17 LPL activity was measured in an aqueous extract of the powder.…”

Section: Assay Of Lpl Activity In Adipose Tissuementioning

confidence: 99%

“…4 -7 Benoit et al 4 reported that in vivo administration of estradiol to female rats potentiated the lipolytic action of epinephrine in fat cells. They suggested that the potentiation of epinephrine-stimulated lipolysis in fat cells by estradiol was likely to represent a major cause to the estradiol-induced depletion of TG.…”

Section: Introductionmentioning

confidence: 99%

The hormonal responses of lipoprotein lipase activity and lipolysis in adipose tissue differ depending on the stage of the estrous cycle in female rats

et al. 2002

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OBJECTIVE: This study was designed to elucidate whether there were differences in the hormonal responses of the parameters involving triacylglycerol (TG) deposition and mobilization in adipose tissue among the stages of the estrous cycle in female rats. MEASUREMENTS: Adipose tissue was obtained from the parametrial region in female rats at each stage of the estrous cycle. Lipoprotein lipase (LPL) activity in the extracts of acetone=ether powders of the tissues was measured as a parameter for TG deposition. Norepinephrine-stimulated lipolysis in isolated fat cells was measured as a parameter for TG mobilization. RESULTS: LPL activity changed periodically during the estrous cycle; the activity level was highest at diestrus, began to decrease at proestrus, reached a minimum at estrus, began to increase again at metestrus-1, and increased further at metestrus-2. At diestrus and proestrus, LPL activity was increased with an increase in plasma insulin levels, suggesting that plasma insulin was the predominant up-regulator of LPL. But at estrus, metestrus-1 and metestrus-2, LPL activity remained low even when plasma insulin levels were high, indicating that it was not up-regulated by plasma insulin. Norepinephrine-stimulated lipolysis in fat cells was high at estrus and metestrus-1 and low at diestrus. CONCLUSION: The hormonal responses of LPL activity and lipolysis in adipose tissue differed depending on the stage of the estrous cycle.

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“…Treatment of female and male rats with E2 has been reported to increase lipolytic responsiveness to catecholamines in isolated adipocytes. 13,28 In humans, a single dose of E2 attenuated catecholamine-stimulated lipolysis in femoral subcutaneous adipose. 7 In our hands, CL was equally effective in increasing whole-body lipolysis in SHAM, OVX and OVX E2 rats.…”

Section: Discussionmentioning

confidence: 99%

“…10 In visceral adipocytes isolated from OVX animals, basal and catecholamine-stimulated lipolysis are lower compared with controls, which is restored after 4-30 d of E2 replacement. 8,[11][12][13] Few studies have explored the relationship between E2 status and subcutaneous adipose tissue lipolysis. In adipocytes isolated from subcutaneous abdominal tissue in humans, Epi-stimulated lipolysis is attenuated in the presence of 100 nM E2 for 24 hours.…”

Section: Introductionmentioning

confidence: 99%

Estradiol does not directly regulate adipose lipolysis

et al. 2017

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The mechanisms by which estradiol modulates adipose lipolysis are poorly understood. We sought to measure basal and b 3 -stimulated indices of lipoysis (FFAs, glycerol) in vivo in E2 deficient or supplemented rats, and ex vivo with direct acute E2 exposure. For 2 weeks, ovariectomized (OVX) and OVX rats treated with a daily oral dose of E2 (OVX E2) were pairfed to SHAM controls (n D 12 per group). Adipocyte size was modestly (»40%) increased in OVX rats, but did not reach significance (p D 0.2). After 2 weeks, half of the animals in each group received an in vivo injection of saline or 1 mg/kg of the b3 agonist CL 316, 243. Serum FFA concentrations, but not glycerol, were lower in OVX and OVX E2 rats compared with SHAM controls (p D 0.02). A significant CL response was present in all groups (p<0.001) and HSL activation was unaffected by OVX or OVX E2 in retroperitoneal (r.p.) or inguinal (iWAT) adipose depots in vivo. Ex vivo, CL increased FFA and glycerol accumulation in the media as well as HSL phosphorylation by several fold in r.p. and iWAT explants, but responses from OVX and OVX E2 rats were comparable to SHAMs. To assess whether E2 can directly affect lipolysis, r.p. and iWAT tissue was treated with E2, CL or E2 C CL for 2, 4 or 8 hours using adipose tissue organ culture. CL stimulated FFA release (p<0.001), but was unaffected by E2. Overall, our results indicate that E2 does not directly regulate adipose tissue lipolysis.

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Potentiation of epinephrine-induced lipolysis in fat cells from estrogen-treated rats

Cited by 39 publications

References 13 publications

Estradiol-Induced Inhibition of Body Weight Gain and Reduction of Food Intake Are Not Associated with Diurnal Rhythmicity.

Estradiol-Induced Inhibition of Body Weight Gain and Reduction of Food Intake Are Not Associated with Diurnal Rhythmicity.

The hormonal responses of lipoprotein lipase activity and lipolysis in adipose tissue differ depending on the stage of the estrous cycle in female rats

Estradiol does not directly regulate adipose lipolysis

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