Potentiation by cigarette smoke of macrophage function against Leishmania donovani infection

Maity, Palash C.; Bhattacharjee, Surajit; Majumdar, S.; Sil, Alok Kumar

doi:10.1007/s00011-008-8151-1

Cited by 15 publications

(10 citation statements)

References 35 publications

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“…Additionally, others have shown the macrophage viability remains relatively high after tobacco smoke exposure (low doses only), however, macrophage inflammatory functions (e.g. oxidative stress production/cytokine release) are activated due to the exposure conditions (Maity et al, 2009). Our results agree with these findings and may provide a pathway for altered inflammatory disease progression due to both heightened inflammatory responses and a depletion of inflammatory cells, both which could lead to inflammatory disease responses.…”

Section: Kupffer Cell Viability and Densitysupporting

confidence: 93%

Tobacco and e-cigarette products initiate Kupffer cell inflammatory responses

Rubenstein

Hom

Ghebrehiwet

2015

Molecular Immunology

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Section: Kupffer Cell Viability and Densitysupporting

confidence: 93%

Tobacco and e-cigarette products initiate Kupffer cell inflammatory responses

Rubenstein

Hom

Ghebrehiwet

2015

Molecular Immunology

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“…Activated macrophages exhibit increased adhesion to and spreading over substrates, altered phagocytic activity and increased ROS generation (Bilitewski et al, 2008). Studies have demonstrated that different drugs and bioproducts induce macrophage activation (Pereira et al, 2005;Lopes et al, 2006;Morrow et al, 2007;Maity et al, 2009;Tiwari and Kakkar, 2009). The present study tested whether KA, which is a secondary metabolite produced by fungi, induces macrophage activation.…”

Section: Discussionmentioning

confidence: 99%

Kojic acid, a secondary metabolite fromAspergillussp., acts as an inducer of macrophage activation

Rodrigues¹,

Carvalho

Santos

et al. 2011

Cell. Biol. Int.

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KA (kojic acid) is a secondary metabolite isolated from Aspergillus fungi that has demonstrated skin whitening, antioxidant and antitumour properties among others. However, limited information is available regarding its effects on macrophages, the major cell involved in cell defence. The aim of the present study was to analyse whether KA affects functional properties related to macrophage activation, such as phagocytosis and spreading ability over a substrate. Treatment of resident macrophages with 50 μg/ml KA for 1 h induced both morphological and physiological alterations in cells. Immunofluorescence microscopy revealed enhanced cell spreading and an increase in cell surface exposure, associated with a rearrangement of microtubules, actin filaments and intermediate filaments. KA also potentiated phagocytosis by macrophages, as demonstrated by the increase in phagocytic activity towards yeast, when compared to untreated cells. KA increased the production of ROS (reactive oxygen species), but not NO (nitric oxide) production. Three tests were used to assess cell viability; MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], NR (neutral red) uptake and PI (propidium iodide) exclusion test, which showed that macrophages maintain their viability following KA treatment. Results indicate that KA can modulate macrophage activation through cytoskeleton rearrangement, increase cell surface exposure, enhance the phagocytic process and ROS production. The study demonstrates a new role for KA as a macrophage activator.

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“…As far as we know, this study constitutes the first report about antileishmanial potentialities of Capsicum and Nicotiana genera. Capsicum frutescens L. extracts showed antiparasitic activity against a fish ectoparasite (Ling et al ., ), and a pre‐treatment with cigarette smoke extract from Nicotiana caused a decrease in parasite burden of Leishmania donovani ‐infected macrophages (Maity et al ., ). In the case of B. cestroides and C. nocturnum , no studies of these species have been found.…”

Section: Resultsmentioning

confidence: 97%

In Vitro Assessment of Plants Growing in Cuba Belonging to Solanaceae Family Against Leishmania amazonensis

Monzote

Jiménez²,

Cuesta‐Rubio

et al. 2016

Phytotherapy Research

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In this study, an in vitro antileishmanial assessment of plant extracts from 12 genera and 46 species growing in Cuba belonging to Solanaceae family was performed. A total of 226 extracts were screened against promastigotes of Leishmania amazonensis, and cytotoxicity of active extracts [median inhibitory concentration (IC ) promastigotes <100 µg/mL] was determined on peritoneal macrophage from BALB/c mice. Extracts that showed selective index >5 were then assayed against intracellular amastigote. Metabolomics analysis of promissory extracts was performed using chemical profile obtained by ultra performance liquid chromatography. Only 11 extracts (4.9%) from nine plants were selected as potentially actives: Brunfelsia cestroides A. Rich, Capsicum annuum L., Capsicum chinense Jacq., Cestrum nocturnum L., Nicotiana plumbaginifolia Viv., Solanum havanense Jacq., Solanum myriacanthum Dunal, Solanum nudum Dunal and Solanum seaforthianum And., with IC < 50 µg/mL and selectivity index >5. Metabolomics analysis demonstrated significant differences in the chemical profiles with an average of 42.8 (range 31-88) compounds from m/z 104 to 1477, which demonstrated the complex mixture of compounds. In addition, no common markers among active extracts were identified. The results demonstrate the importance of the Solanaceae family to search new antileishmanial agents, particularly in unexplored species of this family. Copyright © 2016 John Wiley & Sons, Ltd.

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Potentiation by cigarette smoke of macrophage function against Leishmania donovani infection

Abstract: Low concentration of cigarette smoke extract (CSE) counteracts L. donovani infection-mediated suppression of macrophage function without affecting host cell viability. This study reveals a new role of cigarette smoke extract (CSE) as an activator of macrophage function.

Cited by 15 publications

References 35 publications

Tobacco and e-cigarette products initiate Kupffer cell inflammatory responses

Tobacco and e-cigarette products initiate Kupffer cell inflammatory responses

Kojic acid, a secondary metabolite fromAspergillussp., acts as an inducer of macrophage activation

In Vitro Assessment of Plants Growing in Cuba Belonging to Solanaceae Family Against Leishmania amazonensis

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