Potentials and limitations of quantification of fungi in freshwater environments based on PLFA profiles

Taube, Robert; Fabian, Jenny; Wyngaert, Silke Van den; Agha, Ramsy; Baschien, Christiane; Gerphagnon, Mélanie; Kagami, Maiko; Krüger, Angela; Premke, Katrin

doi:10.1016/j.funeco.2019.05.002

Cited by 16 publications

(18 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PLFAs 18:1ω9t and 18:1ω7c could not be separated chromatographically, thus they will be referred to as 18:1ω9t/7c further on. One cluster was composed of the PLFAs 18:1ω9t/7c and 18:2ω6,9, which are known to be present in daphnia [ 65 ] and fungal biomass [ 54 ]. Thus, these two PLFAs were used as indicators of eukaryotic biomass.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter

et al. 2021

Self Cite

View full text Add to dashboard Cite

Carbon turnover in aquatic environments is dependent on biochemical properties of organic matter (OM) and its degradability by the surrounding microbial community. Non-additive interactive effects represent a mechanism where the degradation of biochemically persistent OM is stimulated by the provision of bioavailable OM to the degrading microbial community. Whilst this is well established in terrestrial systems, whether it occurs in aquatic ecosystems remains subject to debate. We hypothesised that OM from zooplankton carcasses can stimulate the degradation of biochemically persistent leaf material, and that this effect is influenced by the daphnia:leaf OM ratio and the complexity of the degrading microbial community. Fresh Daphnia magna carcasses and 13C-labelled maize leaves (Zea mays) were incubated at different ratios (1:1, 1:3 and 1:5) alongside either a complex microbial community (<50 µm) or solely bacteria (<0.8 µm). 13C stable-isotope measurements of CO2 analyses were combined with phospholipid fatty acids (PLFA) analysis and DNA sequencing to link metabolic activities, biomass and taxonomic composition of the microbial community. Our experiments indicated a significantly higher respiration of leaf-derived C when daphnia-derived OM was most abundant (i.e. daphnia:leaf OM ratio of 1:1). This process was stronger in a complex microbial community, including eukaryotic microorganisms, than a solely bacterial community. We concluded that non-additive interactive effects were a function of increased C–N chemodiversity and microbial complexity, with the highest net respiration to be expected when chemodiversity is high and the degrading community complex. This study indicates that identifying the interactions and processes of OM degradation is one important key for a deeper understanding of aquatic and thus global carbon cycle.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Microbial cell enumeration can be difficult, particularly when considering partially particulate and colloidal OM sources such as daphnia carcasses. Previous studies demonstrated a clear suitability of PLFAs for this purpose [ 10 , 54 , 55 ]. Thus, we used PLFAs as an indicator for microbial biomass.…”

Section: Methodsmentioning

confidence: 99%

Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similarly, during the B. emersonii zoospore-germling transition glycolipids decrease and phospholipids increase ( Dalley and Sonneborn, 1982 ). Previous research has characterised fatty acid profiles in chytrids ( Akinwole et al, 2014 ; Gerphagnon et al, 2019 ; Rasconi et al, 2020 ) and shown differences between chytrid zoospores and sessile thalli of the same species ( Taube et al, 2019 ). As the Nile Red emission spectrum undergoes a red shift in increasingly polar environments ( Bertozzini et al, 2011 ), we propose that our live-cell data do not quantify the structural degradation of the lipid globule per se but rather biochemical polarisation as neutral storage lipids are catabolised and polar phospholipids are synthesised.…”

Section: Discussionmentioning

confidence: 99%

A cellular and molecular atlas reveals the basis of chytrid development

Laundon

Chrismas

Bird

et al. 2022

eLife

View full text Add to dashboard Cite

The chytrids (phylum Chytridiomycota) are a major fungal lineage of ecological and evolutionary importance. Despite their importance, many fundamental aspects of chytrid developmental and cell biology remain poorly understood. To address these knowledge gaps, we combined quantitative volume electron microscopy and comparative transcriptome profiling to create an ‘atlas’ of the cellular and molecular basis of the chytrid life cycle, using the model chytrid Rhizoclosmatium globosum. From our developmental atlas, we describe the transition from the transcriptionally inactive free-swimming zoospore to the more biologically complex germling, and show that lipid processing is multifaceted and dynamic throughout the life cycle. We demonstrate that the chytrid apophysis is a compartmentalised site of high intracellular trafficking, linking the feeding/attaching rhizoids to the reproductive zoosporangium, and constituting division of labour in the chytrid cell plan. We provide evidence that during zoosporogenesis, zoospores display amoeboid morphologies and exhibit endocytotic cargo transport from the interstitial maternal cytoplasm. Taken together, our results reveal insights into chytrid developmental biology and provide a basis for future investigations into non-dikaryan fungal cell biology.

show abstract

“…So far, studies quantifying the ecological significance of phytoplankton-infecting chytrids in aquatic environments are scarce (e.g. Rasconi et al 2009, Taube et al 2019). Yet, it is generally assumed that the parasites play not only an important role in their host population dynamics causing a significant impact on the planet's carbon cycle (e.g.…”

Section: Phytoplankton-infecting Chytridsmentioning

confidence: 99%

Early-diverging fungal phyla: taxonomy, species concept, ecology, distribution, anthropogenic impact, and novel phylogenetic proposals

et al. 2021

View full text Add to dashboard Cite

The increasing number of new fungal species described from all over the world along with the use of genetics to define taxa, has dramatically changed the classification system of early-diverging fungi over the past several decades. The number of phyla established for non-Dikarya fungi has increased from 2 to 17. However, to date, both the classification and phylogeny of the basal fungi are still unresolved. In this article, we review the recent taxonomy of the basal fungi and re-evaluate the relationships among early-diverging lineages of fungal phyla. We also provide information on the ecology and distribution in Mucoromycota and highlight the impact of chytrids on amphibian populations. Species concepts in Chytridiomycota, Aphelidiomycota, Rozellomycota, Neocallimastigomycota are discussed in this paper. To preserve the current application of the genus Nephridiophaga (Chytridiomycota: Nephridiophagales), a new type species, Nephridiophaga blattellae, is proposed.

show abstract

Potentials and limitations of quantification of fungi in freshwater environments based on PLFA profiles

Cited by 16 publications

References 69 publications

Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter

Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter

A cellular and molecular atlas reveals the basis of chytrid development

Early-diverging fungal phyla: taxonomy, species concept, ecology, distribution, anthropogenic impact, and novel phylogenetic proposals

Contact Info

Product

Resources

About