Potentially zoonotic shiga toxin-producing Escherichia coli serogroups in the faeces and meat of food-producing animals in Ibadan, Nigeria

Ojo, Olufemi Ernest; Ajuwape, A.T.P.; Otesile, E. B.; Owoade, A. A.; Oyekunle, M. A.; Adetosoye, A. I.

doi:10.1016/j.ijfoodmicro.2010.06.030

Cited by 69 publications

(60 citation statements)

References 76 publications

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“…The detection of eaeA gene in E. coli O157:H7 isolates in the current study was consistent with 54.7% reported by Ojo, Ajuwape [60] in Nigeria. The presence of this gene in STEC strains has been strongly correlated to their implication in severe illness resulting in HUS [60,61].…”

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confidence: 91%

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Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Ahmed

MacLeod²,

Bayomi³

et al. 2017

Zagazig Veterinary Journal

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A total of 550 meat samples (300 minced beef and 250 chicken meat) marketed in Zagazig City, Sharkia Governorate, Egypt, as well as 150 human stool samples were examined for Shiga toxin producing E. coli. Results revealed that the isolation rates of E. coli O157:H7 versus non-O157 were 1.7% versus 2.3% in minced beef, 0.8% versus 2% in chicken meat and 0.7% versus 2.7% in human stools. Other identified serotypes were including O111:H8 (25%), O26:H11 (20.8%), O55:H7 (16.7%) and O113:H21 (4.2%). Virulence associated genes were identified in E. coli serotypes, stx1 and stx2 were characterized in 16.7% and 62.5% of the isolates, while, eaeA and hlyA genes were identified in 50% and 70.8% of the examined serotypes, respectively. Genotyping of E. coli O157:H7 serotype from different sources using Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) fingerprinting revealed heterogenicity of the isolates, however, human and minced beef isolates were grouped in the same cluster indicating potential transmission of infection from contaminated beef to human consumers. In conclusion, ERIC-PCR is a highly discriminatory, reliable and cost-effective tool for tracing sources of infection with bacteria. Public health education and application of strict hygienic measures during slaughtering, transportation and preparation of meat are essential to minimize the risk of contamination and transmission of infection to consumers.

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supporting

confidence: 91%

“…The presence of this gene in STEC strains has been strongly correlated to their implication in severe illness resulting in HUS [60,61]. However, some STEC strains that are eaeA negative also cause sporadic HUS cases [60,62].…”

mentioning

confidence: 99%

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Ahmed

MacLeod²,

Bayomi³

et al. 2017

Zagazig Veterinary Journal

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“…Stx2-producing strains are often more related to HUS than stx1-producing strains 4,6,33 . In this study, the predominant stx type found was stx2, in aggrement with previous studies 2, 28,32 . The presence of a combination eae, stx and hlyA genes is generally regarded as a highly virulent genetic mix 34 .…”

Section: Discussionsupporting

confidence: 90%

“…This may result from the differences in seasons, ages of animals, method of breeding and geographic differences. It was reported that also sampling method and isolation technique affect prevalence 28,32 . It is reported that IMS method is one of the most sensitive methods for STEC O157 isolation from faeces and food samples 12,13 .…”

Section: Discussionmentioning

confidence: 99%

Elazığ’da Mezbahalarda Kesilen Sığırlardan ve Piyasada Satılan Kıymalardan Shiga Toksin Üreten Escherichia coli O157’nin İzolasyonu, Virulens Genleri ve Antibiyotiklere Duyarlılıkları

Kalender¹

2013

Kafkas Univ Vet Fak Derg

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SummaryShiga Toxin-Producing Escherichia coli O157 (STEC O157) is a foodborne pathogen. Contaminated meat and meat products have an important role in human STEC O157 outbreaks. The aims of this study were to investigate the presence of STEC O157 in slaughtered cattle in abattoirs and ground beef sold in Elazığ, and to determine virulence genes and antimicrobial resistance patterns of STEC O157 isolates. A total of 540 rectal swab samples were collected immediately after slaughter. In addition, 100 ground beef samples were obtained from the butcher shops. Selective enrichment, immunomagnetic separation and plating on Sorbitol MacConkey Agar with cefixime and tellurite (CT-SMAC Agar) were used for the culture. Presence of genes encoding shiga toxin 1 and 2 (stx1 and stx2), H7 flagella (fliCh7), enterohemolysin (hlyA), intimin (eae) and O157 (rfbE) in the isolates was detected by Polymerase Chain Reaction (PCR). In the PCR analysis of rectal swab samples., 34 of 82 sorbitol negative isolates were positive for E. coli O157. 22 (64.7%) of E. coli O157 isolates belonged to E. coli O157:H7. STEC O157 was detected in 18 (3.3%) of rectal swab samples. STEC O157:H7 was isolated from 2 (2%) of ground beef samples. All STEC O157 isolates contained hlyA and eae genes. All STEC O157 isolates obtained from both rectal swab and ground beef samples were resistant to four or more antimicrobials. All STEC O157 isolates were resistant to penicillin, clindamycin, tiamulin and tilmicosin. Two STEC isolates were resistance to ampicillin. Six STEC O157 isolates were resistance to chlortetracycline and sulphadimethoxine. One STEC O157 isolate was resistant to enrofloxacin, florfenicol and oxytetracycline. Keywords: E. coli O157, Cattle, Ground beef, Virulence genes, Antibiotic resistance Elazığ'da Mezbahalarda Kesilen Sığırlardan ve Piyasada Satılan Kıymalardan Shiga Toksin Üreten Escherichia coli O157'nin İzolasyonu, Virulens Genleri ve Antibiyotiklere Duyarlılıkları ÖzetShiga toxin üreten Escherichia coli O157 (STEC O157) gıda kaynaklı enfeksiyonlara yol açan bir patojendir. Kontamine et ve et ürünleri insanlarda salgınların görülmesinde önemli bir rol oynamaktadır. Bu çalışma Elazığ'da mezbahalarda kesilen sığırlardan ve piyasada satılan kıymalardan STEC O157'nin izolasyonu, izolatların virulens genlerinin ve antibiyotiklere duyarlılıklarının belirlenmesi amacıyla yapılmıştır. Kesimden sonra 540 rektal swap örneği ve kasaplardan 100 kıyma örneği toplanmıştır. Etken izolasyonu için selektif zenginleştirme ve immunomagnetik separasyondan sonra sefiksim ve tellürit suplementi içeren Sorbitol MacConkey Agar kullanıldı. Polimeraz zincir reaksiyonu(PCR) ile izolatlarda shiga toxin 1 ve 2 (stx1 and stx2), H7 flagella (fliCh7), enterohemolysin (hlyA), intimin (eae) ve O157 (rfbE) genlerinin varlığı araştırıldı. Rektal sıvap örneklerinin PCR testinde, 82 sorbitol negatif izolatın 34'ü Escherichia coli O157 yönünden pozitif bulundu. Escherichia coli O157 izolatlarının 22'si (%64.7) Escherichia coli O157:H7 olarak tiplendirildi. Rektal swap örn...

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“…The broad range of detection of STEC was reported in other investigations in Germany (56.1%), Spain (47.7%), Bangladesh (10.0%), and Nigeria (7.5%) (28)(29)(30)(31). In the current study, the genotypic profile stx1 had the most frequency (12%) followed by stx1/stx2 (4%), eae (2.8%), stx1/eae (0.8%), and stx2 (0.4%) which is comparable with other studies carried out by other researchers; Oliveira et al (32) reported the genetic profiles stx1, stx2, and stx1/stx2, with the prevalence rates of 24.1%, 34.7%, and 41.2%, respectively.…”

Section: Discussionmentioning

confidence: 59%

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

Jajarmi¹,

Ghanbarpour²,

Sharifi³

et al. 2015

Int J Enteric Pathog

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Background: Escherichia coli can be categorized into four major phylogenetic groups (A, B1, B2, and D) based on presence or absence of three markers including two genes (chuA and yjaA) and an anonymous DNA fragment designated TSPE4.C2. Also, these groups are divided into seven phylogenetic subgroups A 0 , A 1 , B1, B2 2 , B2 3 , D 1 , and D 2 . Objectives: This study aimed to determine the distribution pattern of phylogenetic groups in Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic Escherichia coli (EPEC) isolated from asymptomatic goats in Kerman city, Iran. Materials and Methods: Two hundred and fifty fecal samples were obtained from healthy goats. All isolates were subjected to detection of phylogenetic markers chuA, yjaA and DNA fragment TspE4.C2 and virulence genes stx1, stx2 and eae. Results: In summary, among all isolates phylo-group B1 was the most prevalent (57.6%) and other phylo-groups were A 1 (20.4%), A 0 (18.4%), D 1 (2.8%), and B2 2 (0.8%). There was no isolate in B2 3 and D 2 subgroups. Fifty samples (20%) possessed at least one of the tested virulence genes: stx1 (12%), stx1/stx2 (4%), eae (2.8%), stx1/eae (0.8%), and stx2 (0.4%). Thus, 41 (16.4%) STEC, 7 (2.8%) EPEC, and 2 (0.8%) Enterohemorrhagic Escherichia coli (EHEC) strains were isolated and allocated into four phylogenetic subgroups A 0 (16%), A 1 (12%), B1 (68%), and D 1 (4%). Conclusions: Based on 250 fecal samples obtained from goats in industrial slaughterhouse of Kerman City, goats may be a potential reservoir of STEC in Kerman and B1 followed by A are the most prevalent phylogenetic groups among STEC and non-STEC isolates in this study.

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Potentially zoonotic shiga toxin-producing Escherichia coli serogroups in the faeces and meat of food-producing animals in Ibadan, Nigeria

Cited by 69 publications

References 76 publications

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

Elazığ’da Mezbahalarda Kesilen Sığırlardan ve Piyasada Satılan Kıymalardan Shiga Toksin Üreten Escherichia coli O157’nin İzolasyonu, Virulens Genleri ve Antibiyotiklere Duyarlılıkları

Distribution Pattern of EcoR Phylogenetic Groups Among Shiga Toxin-Producing and Enteropathogenic Escherichia coli Isolated From Healthy Goats

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