Potentially pathogenic Escherichia coli in healthy, pasture-raised sheep on farms and at the abattoir in Brazil

Diversity of Shiga toxin-producing Escherichia coli in sheep flocks of Paraná State, southern Brazil

“…Interestingly, the most common STEC serotypes O76:H19 and O65:H-identified in this study were not frequently recovered from sheep in other geographical areas (Vettorato et al, 2009;Mora et al, 2011;Amé zquita-Ló pez et al, 2012;Schilling et al, 2012;Maluta et al, 2014). Serotype O65:H-is rarely reported in STEC strains from different sources, whereas O76:H19 is often associated with STEC strains of caprine origin (Martin and Beutin, 2011;Mora et al, 2011).…”

“…Nevertheless, only a few studies have reported the occurrence of STEC in Brazilian sheep populations (Vettorato et al, 2009;Maluta et al, 2014;Martins et al, 2014) and, particularly in the South region where approximately 30% of the national flock is found, these data are unknown so far. Thus, the aim of this study was to investigate the occurrence of STEC in sheep flocks from Paraná State, southern Brazil, to better understand the role played by these animals as reservoir of potentially pathogenic strains.…”

Dynamics of extended-spectrum cephalosporin resistance in pathogenic Escherichia coli isolated from diseased pigs in Quebec, Canada

“…For DNA preparation, individual colonies on MacConkey agar plates (Oxoid Company, Nepean, ON, Canada) were inoculated into 5 mL of Luria Bertani (Difco, Mississauga, ON, Canada) broth and were incubated overnight at 37°C. DNA templates were prepared from E. coli isolates by boiled cell lysis for examination by PCR as described previously [9].…”

Impact of medicated feed along with clay mineral supplementation on Escherichia coli resistance to antimicrobial agents in pigs after weaning in field conditions

“…Briefly, floor fecal samples were diluted to 1/10 (weight-for-weight) in buffered peptone water and 200 μl of diluted fecal samples and rectal swabs were inoculated into 5 ml Luria Bertani broth (LB -Difco, USA) and incubated overnight at 37°C. DNA templates were prepared from the processed samples by boiled cell lysis for examination by PCR, as described previously by Maluta et al (Maluta et al, 2014).…”