Potentially Pathogenic Bacteria and Antimicrobial Resistance in Bioaerosols from Cage-Housed and Floor-Housed Poultry Operations

Just, Natasha; Létourneau, Valérie; Kirychuk, Shelley; Singh, Baljit; Duchaine, Caroline

doi:10.1093/annhyg/mer105

Cited by 20 publications

(10 citation statements)

References 36 publications

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“…Most research currently focuses on the search for microorganisms in the aquatic environment. However, there is also growing interest in the presence of antibiotic resistance genes among bioaerosol microorganisms in work premises [ 77 , 78 , 79 ]. It is worth noting the potential of microorganisms in office bioaerosols, which, with their concomitant biofilm capacity, may inhabit air filters in air conditioners or other available moist surfaces.…”

Section: Resultsmentioning

confidence: 99%

Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland

Brągoszewska

Biedroń

2018

IJERPH

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The aims of this article are to characterize: the quantity of culturable bacterial aerosol (QCBA) and the quality of culturable bacterial aerosol (QlCBA) in an office building in Southern Poland during the spring. The average concentration of culturable bacterial aerosol (CCBA) in this building ranged from 424 CFU m−3 to 821 CFU m−3, below Polish proposals for threshold limit values. Size distributions were unimodal, with a peak of particle bacterial aerodynamic diameters less than 3.3 μm, increasing potentially adverse health effects due to their inhalation. The spring office exposure dose (SPED) of bacterial aerosol was estimated. The highest value of SPED was in April (218 CFU kg−1), whereas the lowest was in June (113 CFU kg−1). Analysis was undertaken to determine the antibiotic resistance of isolated strains and their ability to form biofilms, which may facilitate the spread of antibiotic resistance genes. In the course of the study, it was found that Staphylococcus xylosus had the greatest ability to form biofilms, while the strains with the highest antibiotic resistance were Micrococcus luteus D and Macrococcus equipercicus. Given that mainly antibiotic-sensitive bacteria from bioaerosol were isolated, which transfers resistance genes to their plasmids, this shows the need for increased monitoring of indoor air quality in workplaces.

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Section: Resultsmentioning

confidence: 99%

Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland

Brągoszewska

Biedroń

2018

IJERPH

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“…Hence, they demonstrated that antibiotic preparations might act as delivery systems for their cognate ARG. Just et al [ 148 ] found bacitracin-resistance, erythromycin-resistance, and tetracycline resistance genes in bioaerosols from poultry operations, particularly in floor-housed farms. Also, genes associated with erythromycin and quinupristin-dalfopristin resistance, including erm B, erm A, msr C, and msr A/B, as well as mobile genetic elements related to the conjugative transposon Tn916 , have been found in bacterial isolates recovered from flies collected at poultry feeding operations [ 142 ].…”

Section: Antibiotic Resistance and Antibiotic-resistant Bacteria Imentioning

confidence: 99%

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Granados-Chinchilla

Rodríguez

2017

Journal of Analytical Methods in Chemistry

243

128

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Antibiotics are widely used as growth promoters in animal husbandry; among them, the tetracyclines are a chemical group of relevance, due to their wide use in agriculture, surpassing in quantities applied almost every other antibiotic family. Seeing the considerable amounts of tetracyclines used worldwide, monitoring of these antibiotics is paramount. Advances must be made in the analysis of antibiotics to assess correct usage and dosage of tetracyclines in food and feedstuffs and possible residues in pertinent environmental samples. The tetracyclines are still considered a clinically relevant group of antibiotics, though dissemination of tolerance and resistance determinants have limited their use. This review focuses on four different aspects: (i) tetracyclines, usage, dosages, and regulatory issues that govern their food-related application, with particular attention to the prohibitions and restrictions that several countries have enforced in recent years by agencies from both the United States and the European Union, (ii) analytical methods for tetracyclines, determination, and residues thereof in feedstuffs and related matrices with an emphasis on the most relevant and novel techniques, including both screening and confirmatory methods, (iii) tetracycline resistance and tetracycline-resistant bacteria in feedstuff, and (iv) environmental and health risks accompanying the use of tetracyclines in animal nutrition. In the last two cases, we discuss the more relevant undesirable effects that tetracyclines exert over bacterial communities and nontarget species including unwanted effects in farmers.

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“…Our study showed that air sampling indoor air of poultry houses together with shotgun metagenomic sequencing can be used to detect pathogenic microorganisms. This approach can be used to study a various of pathogens, moulds, antimicrobial resistence in environments where a high hygiene standard is required [19, 47]. These findings indicate that in l.e.…”

Section: Discussionmentioning

confidence: 99%

Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

Haverkamp

Spilsberg

et al. 2021

Preprint

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Background: Foodborne pathogens such as Campylobacter jejuni are responsible for a large fraction of the gastrointestinal infections worldwide associated with poultry meat. Campylobacter spp. can be found in the chicken fecal microbiome and can contaminate poultry meat during the slaughter process. The current standard methods to detect these pathogens at poultry farms use fecal dropping or boot swaps in combination with cultivation / PCR. In this study, we have used air filters in combination with shotgun metagenomics for the detection of Campylobacter in poultry houses and MOCK communities to test the applicability of this approach for the detection of foodborne pathogens. Results: The spiked MOCK communities showed that we could detect as little as 200 CFU Campylobacter per sample using our protocols. Since we were interested in detecting Campylobacter, a DNA extraction protocol for Gram negative bacteria was chosen, and as expected, we found that the DNA extraction protocol created a substantial bias affecting the community composition of the MOCK communities. It can be expected that the same bias is present for poultry house samples analyzed. We observed significant amounts of Campylobacter on the air filters using both real-time PCR as well as shotgun metagenomics, irrespective of the amount of spiked in Campylobacter cells, suggesting that the flocks in both houses harboured Campylobacter spp.. Interestingly, in both houses we find diverse microbial communities present in the indoor air. In addition, have we tested the Campylobacter detection rate using shotgun metagenomics by spiking with different levels of C. jejuni cells in both the mock and the house samples. This showed that even with limited sequencing Campylobacter is detectable in samples with low abundance. Conclusions: These results show that air sampling of poultry houses in combination with shotgun metagenomics can detect and identify Campylobacter spp. present at low levels. This is important since early detection of Campylobacter in food production can help to decrease the number of food-borne infections.

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Potentially Pathogenic Bacteria and Antimicrobial Resistance in Bioaerosols from Cage-Housed and Floor-Housed Poultry Operations

Abstract: Most bacteria are more concentrated and most AMR genes are more prevalent in bioaerosols of FH poultry operations, where growth-promoting antibiotics may be used.

Cited by 20 publications

References 36 publications

Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland

Indoor Air Quality and Potential Health Risk Impacts of Exposure to Antibiotic Resistant Bacteria in an Office Rooms in Southern Poland

Tetracyclines in Food and Feedingstuffs: From Regulation to Analytical Methods, Bacterial Resistance, and Environmental and Health Implications

Detection of Campylobacter in air samples from poultry houses using shot-gun metagenomics – a pilot study

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