Potentially Functional microRNA-mRNA Regulatory Networks in Intestinal Ischemia-Reperfusion Injury: A Bioinformatics Analysis

Abstract

Background: Intestinal ischemia-reperfusion (II/R) injury is a common clinical complication associated with high mortality, for which microRNA (miRNA) drives potentially its pathophysiological progression. MiRNAs regulate different messenger RNAs (mRNAs). However, the regulatory network between miRNAs and mRNAs in intestinal ischemiareperfusion injury is elusive. Methods: We analyzed the different expression of mRNAs and miRNAs in intestinal tissues from patients from three groups (arterial group (group A), venous group (group V), control group (group C)). Common differentially expressed (Co-DE) miRNAs and differentially expressed mRNAs were acquired via concerned analyses among the three groups. Co-DE mRNAs were shared parts of target mRNAs and differentially expression mRNAs. Cytoscape was employed to construct the regulatory network between miRNAs and mRNAs. Gene Ontology (GO) analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway depicted the functions and potential pathway associated with Co-DE mRNAs. Using the STRING and Cytoscape, we found critical mRNAs in the protein-protein interaction (PPI) network. Results: The miRNA-mRNA network comprised 8 Co-DE miRNAs and 140 Co-DE mRNAs. Of note, 140 Co-DE mRNAs were targets of these 8 miRNAs, and their roles were established through the functional exploration via GO analysis and KEGG analysis. PPI network and Cytoscape revealed COL1A2, THY1, IL10, MMP2, SERPINH1, COL3A1, COL14A1, and P4HA1 as the top 8 key mRNAs. Conclusion:This study has demonstrated a miRNA-mRNA regulatory network in intestinal ischemia-reperfusion injury, and explored the key mRNAs and their potential functions. These findings could provide new insight into prognostic markers and therapeutic targets for patients with intestinal ischemia-reperfusion injury in clinical practice.

Integrated Analysis of Ferroptosis and Immunity-Related Genes Associated with Intestinal Ischemia/Reperfusion Injury

“…Small intestine expression profiles of 5 intestinal I/R injury patients (group A) and 5 controls (group C) were downloaded from the ENA at EMBL-EBI under accession number PRJEB45362 ( https://www.ebi.ac.uk/ena/browser/view/PRJEB45362 ). 13 …”

Unveiling the molecular complexity of intestinal ischemia–reperfusion injury through omics technologies

“…Another set of studies employed omics technologies to analyze intestinal tissues obtained from patients afflicted by intestinal IR injury caused by MA embolism or thrombosis, or by venous congestion or incarceration [47][48][49].…”

“…Five articles reported on the application of transcriptomics in human studies on intestinal IR [20,45,47,49,50] (Table 1, part II), of which two made use of the human experimental model [45,50]. Kip et al…”

“…Three articles reported on transcriptome data of intestinal tissue of patients suffering from intestinal IR [20,47,49]. The primary investigation was conducted by Jiang et al, encompassing a cohort of eight patients, of which five individuals exhibited arterial prob-lems, specifically embolism or thrombosis, while the remaining three were diagnosed with venous-related issues, such as congestion or incarceration [47]. These patient groups were compared to a control group comprising five surgical patients without intestinal IR, for whom clinical conditions were not specified [47].…”

“…The primary investigation was conducted by Jiang et al, encompassing a cohort of eight patients, of which five individuals exhibited arterial prob-lems, specifically embolism or thrombosis, while the remaining three were diagnosed with venous-related issues, such as congestion or incarceration [47]. These patient groups were compared to a control group comprising five surgical patients without intestinal IR, for whom clinical conditions were not specified [47]. Additionally, information about the specific location of the intestinal tissue sampled and the severity of ischemia was not provided [47].…”