Potential vectors of <i><scp>X</scp>ylella fastidiosa</i>: a study of leafhoppers and treehoppers in citrus agroecosystems affected by Citrus Variegated Chlorosis

Dellapé, Gimena; Paradell, Susana Liria; Semorile, Liliana; Delfederico, Lucrecia

doi:10.1111/eea.12491

Cited by 24 publications

(11 citation statements)

References 41 publications

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“…The bacterium is transmitted to plants by xylem-sap feeding leafhoppers (Hemiptera, Cicadomorpha) and members of several families are known to transmit the disease from plants to plants 6 . In the Americas, Cicadellidae 15 , 16 , spittlebugs (Cercopidae, Clastopteridae, Aphrophoridae) 17 – 20 , and cicadas (Cicadidae) 19 , 21 have been shown to efficiently transmit Xf . In Europe, little is known about the vectors that efficiently transmit the bacterium.…”

Section: Introductionmentioning

confidence: 99%

Using insects to detect, monitor and predict the distribution of Xylella fastidiosa: a case study in Corsica

Cruaud

Gonzalez

Martin

et al. 2018

Sci Rep

107

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We sampled ca 2500 specimens of Philaenus spumarius (Hemiptera: Aphrophoridae) throughout Corsica without a priori knowledge on the presence of symptoms on plants. We screened 448 specimens for the presence of Xylella fastidiosa (Xf) using qPCR and a custom nested PCR. qPCR appeared versatile and under-estimated the prevalence of Xf. Nested PCR showed that Xf was present in all populations. Molecular results were validated by prediction on the distribution of Xf made from tests conducted on plants, which shows the pertinence of using vectors in risk assessment studies. Xf was detected in tenerals and adults. Thus, P. spumarius could acquire Xf from its host plant, mostly Cistus monspeliensis in Corsica, which may act as reservoir for the next season. This contrasts with other observations and suggests that management strategies may have to be adapted on a case-by-case basis. At least two genetic entities and several variants of Xf not yet identified on plants were present in the insects, which suggests ancient introductions of Xf and a probable underestimation of the current diversity of the strains present in Corsica. Interestingly 6% of the specimens carried two subspecies of Xf. Studies are required to better characterize the strains present in Corsica and to determine how the disease was introduced, spread and why no sign of a potential epidemic was detected earlier. This study shows that, when sensitive enough methods are implemented, spittlebugs (and more specifically P. spumarius for which species distribution modelling shows it could be a good sentinel for Europe) can be used to predict and better assess the exact distribution of Xf. Furthermore, Xf multiply only in their foregut and does not become circulative, which facilitates its detection.

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Section: Introductionmentioning

confidence: 99%

Using insects to detect, monitor and predict the distribution of Xylella fastidiosa: a case study in Corsica

Cruaud

Gonzalez

Martin

et al. 2018

Sci Rep

107

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“…The bacterium is transmitted to plants by xylem-sap feeding leafhoppers (Hemiptera,74 Cicadomorpha) and members of several families are known to transmit the disease from 75 plants to plants (Redak et al, 2004a;Redak et al, 2004b). In the Americas, Cicadellidae 76 (Yang, 1994;Dellapé et al, 2016), spittlebugs (Cercopidae, Clastopteridae, Aphrophoridae) 77 (Severin, 1949;Severin, 1950;Almeida et al, 2005;Krell et al, 2007), and cicadas 78 (Cicadidae) (Paião et al, 2002;Krell et al, 2007) have been shown to efficiently transmit Xf. 79…”

Section: Introduction 45 46mentioning

confidence: 99%

Using insects to detect, monitor and predict the distribution ofXylella fastidiosa:a case study in Corsica

Cruaud

Gonzalez

Martin

et al. 2018

Preprint

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11We sampled ca 2500 specimens of Philaenus spumarius throughout Corsica without a priori 12 on the presence of symptoms on plants. We screened 448 specimens for the presence of 13Xylella fastidiosa (Xf) using qPCR and a custom nested PCR. qPCR appeared versatile and 14 under-estimated the prevalence of Xf. Nested PCR showed that Xf was present in all 15 populations. Molecular results were validated by prediction on the distribution of Xf made 16 from tests conducted on plants, which shows the pertinence of using vectors in risk 17 assessment studies. Xf was detected in tenerals and adults. Thus, P. spumarius could acquire 18Xf from its host plant, mostly Cistus monspeliensis in Corsica, which may act as reservoir for 19 the next season. This contrasts with other observations and suggests that management 20 strategies may have to be adapted on a case-by-case basis. At least two genetic entities and 21 several variants of Xf not yet identified on plants were present in the insects, which suggests 22 ancient introductions of Xf and a probable underestimation of the current diversity of the 23 strains present in Corsica. Interestingly 6% of the specimens carried two subspecies. Studies 24 are wanted to better characterize the strains present in Corsica and know how the disease was 25 introduced, spread and why no sign of a potential epidemic was detected earlier. This study 26shows that, when sensitive enough methods are implemented, insects can be used to predict 27 and better assess the exact distribution of Xf. Insects are indeed easy to collect, Xf multiply 28 only in their foregut and does not become circulative, which facilitates its detection. 29 30

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“…; Dellapé et al . ). In vineyards in Bahia, Brazil, abundance of Homalodisca spottii (Takiya, Cavichioli, & McKamey) (Hemiptera: Cicadellidae) was affected by trapping height, regional crop pruning practices and composition of nearby habitats (Ringenberg et al .…”

Section: Assessing Vector Abundance and Diversitymentioning

confidence: 97%

Sharpshooters: a review of what moves Xylella fastidiosa

et al. 2019

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Sharpshooters (Hemiptera: Cicadellidae: Cicadellinae) and spittlebugs (Hemiptera: Aphrophoridae: Aphrophorinae) have a worldwide distribution and are often associated with many crops. Because the geographic range of sharpshooters and spittlebugs often overlaps with the range of the plant pathogenic bacterium Xylella fastidiosa, the importance of these groups of insects in spreading X. fastidiosa rises to the level of a major socioeconomic problem. Managing diseases caused by X. fastidiosa is challenging for several reasons. First, multiple sequence types of the pathogen have been identified, with sequence type affecting host plant specificity. Second, multiple vector species may be present in an agroecosystem, and the host range of vector species is often broad. Although multiple vector control options such as biological, chemical, physical and cultural controls have been described, disease control programs must take into account biological and ecological parameters of locally dominant X. fastidiosa sequence types and vector species to develop suitable control strategies. Our review demonstrates how fundamental principles of vector reproductive biology, behaviour, nutrition and population and community ecology have influenced research on insect vectors and provides insights into priority research areas.

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Potential vectors of Xylella fastidiosa: a study of leafhoppers and treehoppers in citrus agroecosystems affected by Citrus Variegated Chlorosis

Cited by 24 publications

References 41 publications

Using insects to detect, monitor and predict the distribution of Xylella fastidiosa: a case study in Corsica

Using insects to detect, monitor and predict the distribution of Xylella fastidiosa: a case study in Corsica

Using insects to detect, monitor and predict the distribution ofXylella fastidiosa:a case study in Corsica

Sharpshooters: a review of what moves Xylella fastidiosa

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