Potential use of superparamagnetic iron oxide nanoparticles for in vitro and in vivo bioimaging of human myoblasts

Abstract

Myocardial infarction (MI) is one of the most frequent causes of death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown to be effective in the failing heart but shows limitations such, e.g. cell retention and survival. We synthesized and investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent for direct cell labeling, which can be used for stem cells imaging. High quality, monodisperse and biocompatible DMSA-coated SPIONs were obtained with thermal decomposition and subsequent ligand exchange reaction. SPIONs’ presence within myoblasts was confirmed by Prussian Blue staining and inductively coupled plasma mass spectrometry (ICP-MS). SPIONs’ influence on tested cells was studied by their proliferation, ageing, differentiation potential and ROS production. Cytotoxicity of obtained nanoparticles and myoblast associated apoptosis were also tested, as well as iron-related and coating-related genes expression. We examined SPIONs’ impact on overexpression of two pro-angiogenic factors introduced via myoblast electroporation method. Proposed SPION-labeling was sufficient to visualize firefly luciferase-modified and SPION-labeled cells with magnetic resonance imaging (MRI) combined with bioluminescence imaging (BLI) in vivo. The obtained results demonstrated a limited SPIONs’ influence on treated skeletal myoblasts, not interfering with basic cell functions.

Bioevaluation of superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with dihexadecyl phosphate (DHP)

“…6). The method was developed and described in our previous study 24 . For TCam-2 cells, the highest intracellular iron concentration (316 ± 18 ppb) was detected at 0.025 mg/mL of SPION-DHP.…”

“…Ligand exchange procedure was performed to exchange the capping ligand from oleic acid to meso-2,3-dimercaptosuccinic acid (DMSA). This method has been described in our previous work 24 . In the first step, 50 mg of DMSA was dissolved in 15 mL dimethylsulfoxide (DMSO) and 100 mg of nanoparticles were diluted in 15 mL of chloroform.…”

Rekindling RNAi Therapy: Materials Design Requirements for In Vivo siRNA Delivery

“…Scale bar represents 50 nm); and superparamagnetic iron oxide nanoparticles (SPION; TEM image adapted from ref. under the terms of the CC BY 4.0 license. Scale bar represents 10 nm); d) mesoporous silica particles are represented by a cross‐sectional view of the spherical porous particle (TEM image adapted with permission .…”

Abrindo a Caixa De Pandora Dos Nanomedicamentos: Há Realmente Muito Mais ‘Espaço Lá Embaixo

“…74 Nanopartículas de óxido de ferro são aplicados para bioimagem, biossensibilização e terapia fototérmica. 75 Nanopartículas de óxido de titânio e óxido de zinco são comumente usadas em produtos como formulações contendo filtro solar. 76 Nanocarreadores e modulação das propriedades físico-químicas e farmacocinéticas de fármacos É importante compreender como os NC podem atuar levando a uma maior eficácia e segurança terapêutica.…”