Potential use of probiotic Lactobacillus plantarum SS2 isolated from a fermented plant beverage: safety assessment and persistence in the murine gastrointestinal tract

Abstract

A strain of Lactobacillus plantarum SS2 isolated from a fermented star fruit (Averrhoa carambola) beverage product in Thailand has been reported to have some probiotic properties, in vitro. In the present study, its in vivo safety and gastrointestinal survival following oral administration to mice was investigated. The acute toxicity test on ICR mice by force-feeding of a single dose of 10 9 and 10 12 cells per mouse over 14 days after ingestion showed no adverse effects related to the normal behavior of the laboratory mouse. Although the weight gains of the dosed mice were lower than the control they were still within normal values. There were no significant differences in liver weight ratio or spleen weight index among tested mice and control mice and no evidence of bacteremia. Live SS2 cells labeled with a fluorescent dye (cFDA-SE) and administered orally at 10 9 cells per mouse were shown to persist in the gastrointestinal tract for 7 days. Colonies similar to the SS2 were detected in fecal samples from the test mice even though the fecal lactic acid bacterial count showed no significant difference in any mice. The strain SS2 is therefore considered to be a possible alternative choice for an inoculum to produce fermented plant beverages.

Safety Assessment of Lactobacillus salivarius REN, a Probiotic Strain Isolated from Centenarian Feces

“…Hematological parameters are usually used to represent the deleterious effects of test substance on the blood (Lee et al, 2010), when the composition of blood cells change, it might indicate the pathological changes in bone marrow or the reaction of immune cells (Yakubu et al, 2007). Biochemical assays are used to detect mild nutrient deficiencies or imbalance in sessment is commonly used in safety evaluation of probiotic (Duangjitcharoen et al, 2009;Perelmuter et al, 2011), thus we conducted a 28-day repeated oral dose study. No noticeable activity or behavioral changes were observed in mice during the experimental protocol, and no illness or death occurred.…”

The Microbiome of Spodoptera littoralis: Development, Control and Adaptation to the Insect Host

“…Fluorescence imaging is commonly associated with the use of green and red fluorescence proteins [31,32]. Rats and mice are popular model organisms which study the proliferation and colonization of lactic acid bacteria (LAB) [33,34]. LAB has been tagged with green fluorescent protein (GFP) and mCherry to study their colonization of the intestinal tract of chickens, mice and zebrafish [35][36][37][38][39].…”

“…pTRKH3 is a shuttle vector for E. coli, Enterococcus, Lactococcus, Streptococcus and Lactobacillus [60]. The vector has a copy number (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) in E. coli, and a somewhat higher copy number in Lactococcus and Streptococcus species [64]. It carries a gene for erythromycin resistance, which is expressed in E. coli and LAB.…”

Evaluation of factors that influence the L-glutamic and γ-aminobutyric acid production duringHericium erinaceusfermentation by lactic acid bacteria

“…FPBs are non-alcoholic and are produced by lactic acid bacteria (LAB) from different edible plants such as medicinal plants, vegetables and fruits (Kantachote & Charernjiratrakul, 2008a, 2008bPrachyakij, Charernjiratrakul, & Kantachote, 2008;Ratanaburee, Kantachote, Charernjiratrakul, Penjamras, & Chaiyasut, 2011). Fermented plant juices are well known and are considered to be a novel functional food product since they contain live LAB-based probiotics, plenty of nutrients, antioxidants and antimicrobials (Duangjitcharoen, Kantachote, Ongsakul, Poosaran, & Chaiyasut, 2009;Prachyakij et al, 2008;Ratanaburee et al, 2011).…”